张蕾,倪穗,李纪元,范正琪,蒋昌杰,陈德龙.金花茶不同时期花瓣营养与生物活性成分分析[J].林业科学研究,2019,32(2):32-38
金花茶不同时期花瓣营养与生物活性成分分析
Analysis on the Nutrients and Bioactive Components of Petals in Different Floral Stages of Camellia nitidissima
投稿时间:2018-10-04  修订日期:2018-12-04
DOI:10.13275/j.cnki.lykxyj.2019.02.005
中文关键词:  金花茶  不同发育时期  花瓣  生物活性成分  营养成分
英文关键词:Camellia nitidissima  floral development stages  petals  bioactive contents  nutrients
基金项目:国家政府间国际科技创新合作重点专项(2016YFE0126100);中国林科院中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2017ZF001);宁波市科技富民项目(2017C10050)
作者单位E-mail
张蕾 宁波大学, 浙江 宁波 315832
中国林业科学研究院亚热带林业研究所, 浙江 杭州 311400 
 
倪穗 宁波大学, 浙江 宁波 315832 nbnisui@126.com 
李纪元 中国林业科学研究院亚热带林业研究所, 浙江 杭州 311400 jiyuan_li@126.com 
范正琪 中国林业科学研究院亚热带林业研究所, 浙江 杭州 311400  
蒋昌杰 南宁市金花茶公园, 广西 南宁 530022  
陈德龙 广西源之源生态农业投资有限公司, 广西 南宁 530212  
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中文摘要:
      [目的]研究金花茶花瓣4个典型发育期3大类化学成分的时序动态变化,以确定金花茶花瓣营养与活性成分最适采收期,为金花茶开发利用提供依据。[方法]总黄酮、总多酚采用比色法分析,膳食纤维采用酶解法分析,抗坏血酸、可溶性糖采用色谱法分析,氨基酸采用柱后衍生离子交换色谱法分析,矿质元素采用原子吸收分光光度法分析。[结果]不同发育时期金花茶花瓣总黄酮、总多酚、总膳食纤维、可溶性糖、水解氨基酸和抗坏血酸平均含量分别为11.1%、1.59%、54.8%、22.9%、2.57%和0.294 mg·g-1;在检测的7种常量元素中,主要常量元素为K (12.7 g·kg-1)、Ca (2.97 g·kg-1)、Mg (1.78 g·kg-1),在检测的8种矿质微量元素中,主要微量元素包括Mn (188 mg·kg-1)、Fe (38.4 mg·kg-1)、Zn (5.93 mg·kg-1);Fe和Zn在初谢期最高,而Se在盛开期最高(0.048 9 mg·kg-1)。生物活性成分含量排序为盛开期 > 花蕾期 > 半开期 > 初谢期,其它营养成分排序为盛开期 > 初谢期 > 半开期 > 花蕾期。[结论]金花茶盛开期花瓣营养及活性成分全面,协同性好,适宜开发多种功能性保健食品。
英文摘要:
      [Objective] To determine the best harvest period at the peak of nutrients and active ingredients and to provide solid basis for processing utilization of Camellia nitidissima by studying the dynamic patterns of three chemical components in 4 typical developmental stages of its petals.[Method] Total flavonoids and total polyphenols were determined by colorimetric method, dietary fiber by enzymatic method, ascorbic acid and soluble sugar by chromatography, amino acids by high performance ion exchange chromatography (HPIEC) with post-column derivatization, and mineral elements by atomic absorption spectrometry.[Result] The average content of total flavonoids, total polyphenols, total dietary fiber, soluble sugar, hydrolyzed amino acids and ascorbic acid in the petals of C. nitidissima at different developmental stages were 11.1%, 1.59%, 54.8%, 22.9%, 2.57% and 0.294 mg·g-1, respectively. Among the seven major elements tested, the main macro-elements were K (12.7 g·kg-1), Ca (2.97 g·kg-1), Mg (1.78 g·kg-1). Among the eight mineral trace elements detected, the main trace elements included Mn (188 mg·kg-1), Fe (38.4 mg·kg-1), Zn (5.93 mg·kg-1). Fe and Zn reached their peaks in early withering, while Se had the highest value in blooming period (0.048 9 mg·kg-1). The contents of bioactive components were ranked as below:full blossom > floral bud > half open > early withering. The rank of other nutrients were full blossom > early withering > half open > floral bud.[Conclusion] C. nitidissima petals are rich in the bioactive components and nutrients with good coordination in the full blossom, and are suitable for processing various kinds of functional food products.
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