成星奇,贾会霞,孙佩,张亚红,胡建军.丹红杨×通辽1号杨杂交子代叶形性状的遗传变异分析[J].林业科学研究,2019,32(2):100-110
丹红杨×通辽1号杨杂交子代叶形性状的遗传变异分析
Genetic Variation Analysis of Leaf Morphological Traits in Populus deltoides cl. ‘Danhong’×P. simonii cl. ‘Tongliao 1’ Hybrid Progenies
投稿时间:2018-02-26  修订日期:2019-01-16
DOI:10.13275/j.cnki.lykxyj.2019.02.015
中文关键词:  杨树  子代  叶形性状  遗传变异
英文关键词:Populus  progenies  leaf morphological traits  genetic variation
基金项目:国家自然科学基金面上项目“杨属派间杂种苗期抗旱相关性状QTL定位及新品种选育”(31570669);国家重点研发计划课题“速生人工林木材品质性状的遗传基础”(2017YFD0600201)
作者单位E-mail
成星奇 林木遗传育种国家重点实验室, 国家林业和草原局林木培育重点实验室, 中国林业科学研究院林业研究所, 北京 100091  
贾会霞 林木遗传育种国家重点实验室, 国家林业和草原局林木培育重点实验室, 中国林业科学研究院林业研究所, 北京 100091  
孙佩 林木遗传育种国家重点实验室, 国家林业和草原局林木培育重点实验室, 中国林业科学研究院林业研究所, 北京 100091  
张亚红 林木遗传育种国家重点实验室, 国家林业和草原局林木培育重点实验室, 中国林业科学研究院林业研究所, 北京 100091  
胡建军 林木遗传育种国家重点实验室, 国家林业和草原局林木培育重点实验室, 中国林业科学研究院林业研究所, 北京 100091 hujj@caf.ac.cn 
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中文摘要:
      [目的]对丹红杨(美洲黑杨)×通辽1号杨(小叶杨)422个F1代无性系叶的形态学指标进行分析,揭示杨树派间杂交群体叶形性状的遗传变异规律,为进一步解析其叶形性状的遗传机制奠定基础。[方法]使用Yaxin-1241便携式叶面积仪结合手工测量的方式,对杂交子代当年扦插材料的叶面积、叶长、叶宽、叶周长、叶柄长、侧脉夹角和最大叶宽距叶尖长度等7个指标进行测量,计算叶长宽比、叶柄相对长、最大叶宽位置和叶缘因子,利用SPSS软件完成相关、通径、遗传以及主成分分析。[结果]丹红杨和通辽1号杨两亲本叶面积、叶长、叶宽、叶周长、叶柄长、侧脉夹角、叶长宽比、叶柄相对长以及最大叶宽位置等9个指标差异显著,子代变异丰富且呈连续的正态分布。在10个叶形指标间45对相关关系中,36对呈显著或极显著水平。通径分析结果表明,叶长和叶宽是决定叶面积的主要因素。通过主成分分析得到2个主因子,累计解释80.19%的叶形变异,子代第一主成分的标准化值全部介于两亲本之间,并有64.69%与父本分布在同侧,第二主成分则均匀分布在中亲值两侧。对于表征叶片大小的2个指标叶面积和叶柄长,多数子代小于中亲值且其均值更接近父本,而表征叶片形状的叶柄相对长和最大叶宽位置与之相反,其余指标接近中亲值。除叶长宽比、侧脉夹角以及叶缘因子外,子代叶形指标大多介于两亲本之间。[结论]丹红杨和通辽1号杨叶形性状差异显著,杂交子代叶形性状存在丰富的连续性变异,且性状间关联紧密,叶长和叶宽是决定叶面积的主要因素。叶面积、叶柄长以及最大叶宽位置等性状存在明显的遗传偏向性,杂交子代叶片形状与丹红杨更为接近,呈较强的母本效应,而叶片大小则表现出偏向父本小叶杨的遗传效应。30.09%子代的综合叶形指标具有超亲现象,选择潜力较大,在今后的育种实践中可加以利用。
英文摘要:
      [Objective] To reveal the genetic variation pattern of leaf morphological traits in poplar intersectional hybrid population, and to lay the foundation for studying their genetic mechanisms by analyzing the leaf morphological indexes in 422 F1 progenies from P. deltoides cl. ‘Danhong’×P. simonii cl. ‘Tongliao 1’.[Method] Seven indexes (leaf area, length, width, perimeter, petiole length, vein angle and the distance between largest width and blade tip) were measured through Yaxin-1241 portable area meter and manual measurement. Four indexes (the ratio of leaf length and width, relative length of petiole, position of the largest width and leaf margin factor) were calculated. Correlation, path coefficient, genetic and principal component analysis were accomplished by SPSS software.[Result] Significant differences were found between two parents for all morphology indexes except leaf margin factor. Plentiful phenotypic variations with normal and continuous distribution existed in hybrid population. As many as 36 of 45 pairs of correlations which were up to significant or extremely significant level. The result of path coefficient analysis indicated that the leaf area was determined mainly by leaf length and width. Two main factors were obtained through principal component analysis, which explained 80.19% of total variations. Standardized value of first principal component in progenies was fallen completely between two parents, with 64.69% were distributed on the same side of male parent, while the second principal component was evenly distributed on both sides of the median parents. Two indexes of leaf size (leaf area and petiole length) in progenies were lower than the median of parents, and the mean was closer to male parent. In contrast, the leaf shape indexes (relative length of petiole and the position of maximum blade width) were closer to the female parent. Other indexes were closer to the median of parents. Most indexes of leaf morphology were between two parents except the ratio of length and width, the leaf margin factor and the lateral vein angle.[Conclusion] There are significant differences in leaf morphological traits between two parents. The variation of leaf morphological traits in their progenies is continuous and plentiful, and these traits are closely associated, among which leaf length and leaf width are the main factors determining leaf area. Leaf area, petiole length and position of the largest width have a evident genetic bias, the leaf shape of hybrid offspring which is more similar to P. deltoides cl ‘Danhong’ poplar shows a strong maternal effect, while the leaf size shows a genetic bias toward the male P. simonii cl. ‘Tongliao 1’ parental effect. The comprehensive leaf shape index of 30.09% progenies has the transgressive segregation phenomenon, which has great potential for selection, and could be utilized in tree breeding in the future.
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