胡继文,肖遥,翟文继,卢楠,沈元勤,麻文俊,杨桂娟,王军辉.香椿花粉离体萌发与花粉生活力研究[J].林业科学研究,2019,32(2):160-165
香椿花粉离体萌发与花粉生活力研究
Research on Pollen Germination and Vigor of Toona sinensis
投稿时间:2018-09-03  修订日期:2018-11-28
DOI:10.13275/j.cnki.lykxyj.2019.02.023
中文关键词:  香椿  离体培养条件  花粉萌发率  花药大小  含水率  低温贮藏
英文关键词:Toona sinensis  in vitro culture condition  germination rate  anther size  water content  low temperature storage
基金项目:“十三五”国家重点研发计划课题“红锥、椿木等高效培育技术研究”(2016YFD0600606)
作者单位
胡继文 中国林业科学研究院林业研究所, 北京 100091 
肖遥 中国林业科学研究院林业研究所, 北京 100091 
翟文继 南阳市林业科学研究院, 河南 南阳 473001 
卢楠 中国林业科学研究院林业研究所, 北京 100091 
沈元勤 南阳市林业科学研究院, 河南 南阳 473001 
麻文俊 中国林业科学研究院林业研究所, 北京 100091 
杨桂娟 中国林业科学研究院林业研究所, 北京 100091 
王军辉 中国林业科学研究院林业研究所, 北京 100091 
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中文摘要:
      [目的]探究香椿花粉最佳离体培养条件,花药大小、含水率与花粉萌发率的关系及花粉生活力随低温贮藏时间的变化的规律。[方法]以3个不同基因型(XC5、XC22、ZJ26)香椿花粉为试验材料,观测不同培养温度(20、22、25、28℃)、培养时间(4、6、12、18、30、42 h)下,花粉离体培养的萌发率,获得香椿花粉最佳培养条件。对香椿的花药含水率、花药长、宽进行测量,分析其与花粉萌发率的关系。基于最佳培养条件,检测香椿花粉-20℃低温贮藏10、25、40 d的萌发情况,分析花粉生活力随低温贮藏天数的变化趋势。[结果]香椿花粉在20℃的萌发率均显著高于其余3个培养温度,花粉萌发率随培养时间的延长而增大,培养30 h后,花粉萌发率均无显著变化。低温贮藏10 d的花粉萌发率与花药长度显著正相关,与花药宽度显著负相关,相关系数分别为0.529 6、-0.739 9。随着低温贮藏时间的延长,香椿花粉萌发率显著降低,含水率高的XC22降幅最大,低温贮藏10、25 d的花粉萌发率分别为89.2%、3.3%。[结论]检测香椿花粉生活力的最佳离体培养条件为20℃培养30 h;香椿花粉萌发率与花药长度、宽度以及含水率可能作为筛选花粉高萌发率的指标;低温贮藏可能对含水率高的香椿花粉生活力影响大。
英文摘要:
      [Objective] To study the optimal in vitro culture conditions of Toona sinensis pollen, the correlation between pollen germination rate and anther size and water content, and the variation of pollen vigor in different low temperature storage time.[Method] The pollens from three T.sinensis genotype plants(XC5, XC22, ZJ26) were used as experimental materials to observe the germination rate of pollen in vitro under different culture temperatures(20, 22, 25, and 28℃) and different culture times(4, 6, 12, 18, 30, and 42 hours), in order to obtain the optimal culture conditions.The anther water content, length and width of each genotype were measured. And the relationship between pollen germination and anther water content, length and width was analyzed. The germination on the optimal culture condition of T. sinensis pollen at low temperature (-20℃) for 10, 25 and 40 days were viewed. And the change trend of pollen vigor with low temperature storage time was analyzed.[Result] The germination rate of T. sinensis pollen at 20℃ was significantly higher than that at the other culture temperatures. The pollen germination rate increased with the increase of culture time. After 30 hours, the pollen germination rate did not increase obviously.There was dramatically positive/negative correlation between pollen germination rate and anther length/width, and the R2 were 0.529 6 and -0.739 9, respectively, while the pollen germination rate was not significant positive correlated with the anther water content at low temperature for 10 days. With the extension of low temperature storage time, the pollen germination rate decreased greatly. The pollen germination rates of the XC22, a high water content genotype,in low temperature storage for 10 and 25 days were 89.2%, 3.3%, respectively.[Conclusion] The optimal in vitro culture condition for the pollen vigor of T. sinensis was 20℃ for 30 hours. The correlation between the germination rate of T. sinensis pollen and the size, water content of anther might be used as indicators for screening pollen vigor. Low temperature storage might have a greater influence on the pollen vigor of T. sinensis with high water content.
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