郭丽琴,李友丽,饶国栋,张建国.利用SRAP分子标记分析杨属遗传变异和亲缘关系[J].林业科学研究,2019,32(3):88-96
利用SRAP分子标记分析杨属遗传变异和亲缘关系
Genetic Variation and Genetic Relationship Analysis of Populus Based on SRAP Markers
投稿时间:2018-04-03  修订日期:2019-03-22
DOI:10.13275/j.cnki.lykxyj.2019.03.012
中文关键词:  杨属  SRAP  遗传变异  亲缘关系
英文关键词:Populus  SRAP  genetic variation  genetic relationship
基金项目:中央级公益性科研院所专项资金项目-中国林科院"优秀青年创新人才培育计划"(CAFYBB2018QB001)
作者单位E-mail
郭丽琴 中国林业科学研究院林业研究所, 国家林业和草原局林木培育重点实验室, 北京 100091  
李友丽 北京农业智能装备技术研究中心, 北京 100097  
饶国栋 中国林业科学研究院林业研究所, 国家林业和草原局林木培育重点实验室, 北京 100091  
张建国 中国林业科学研究院林业研究所, 国家林业和草原局林木培育重点实验室, 北京 100091 Zhangjg@caf.ac.cn 
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中文摘要:
      [目的]对采集自不同地区的22个杨属树种33个样本的遗传变异和亲缘关系进行研究。[方法]利用从72对引物中筛选出的40对相关序列扩增多态性(SRAP)引物组合对杨属不同树种进行遗传变异和亲缘关系分析。[结果]40对引物共扩增出487条清晰条带,其中,多态性条带为441条,多态性条带比率为90.55%。利用UPGMA得到的聚类分析结果表明了33个样本间的遗传关系,遗传相似系数为0.448 6~0.910 3;白杨派和胡杨派的派间平均遗传距离最大,为0.377 7。聚类分析结果表明,33个样本可分为4大类,这一聚类结果与传统的分类结果一致。通过系统发育树可以看出,白杨派单独形成一个分支,黑杨派、胡杨派和青杨派形成另一分支,可能暗示白杨派与其他各派的亲缘关系较远,青杨派与黑杨派和胡杨派的亲缘关系则比较近。[结论]不同的杨树品种在SRAP位点有较高的多态性,SRAP分子标记适用于分析杨属的遗传变异和亲缘关系,研究结果为杨属树种的分类鉴定、系统进化等提供了一定的理论依据。
英文摘要:
      [Objective] This paper aims at investigating the genetic variation and genetic relationship of 33 samples of 22 Populus species.[Method] 40 primer combinations screened from 72 of SRAP (Sequence-related Amplified Polymorphism) markers were used to analyze the genetic variation and the genetic relationship of Populus.[Result] The results showed that 40 pairs of primer combinations produced 487 clear bands, and there were 441 polymorphic bands with a ratio of 90.55%. The dendrogram generated by UPGMA showed the genetic relationship among 33 samples. The genetic similarity among 33 samples varied from 0.448 6 to 0.910 3; the maximum average genetic distance among sections in Populus was between Sect. Leuce Duby and Turanga, which was 0.3777. The 33 samples were classified into four major groups by cluster analysis using UPGMA. This result was in accordance with the previous classification based on conventional methods. Phylogenetic tree showed that all samples in Sect. Leuce Duby formed a separate branch, while Sect. Tacamahaca, Aigeiros and Turanga formed another branch, which may indicate that Sect. Leuce Duby have rather distant phylogenetic relationships with the others and the genetic relationship among the other three sections was closer.[Conclusion] Different poplar species have high polymorphism in SRAP sites, and SRAP could be a potentially useful marker technique for genetic variation and genetic relationship study in Populus germplasm. The results would provide a theoretical basis for studying the classification, identification and systematic evolution of Populus.
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