新疆巩留县杏果实斑点病病原菌鉴定
The pathogen identification of apricot fruit spots disease in Gongliu County, Xinjiang
投稿时间:2018-07-16  修订日期:2018-08-22
DOI:
中文关键词:  杏果实斑点病  rDNA-ITS  系统发育学分析  Thyrostroma carpophilum
英文关键词:apricot fruit spots  rDNA-ITS  phylogenetic analysis  Thyrostroma carpophilum
基金项目:国家重点研发计划
作者单位E-mail
程元 中国林业科学研究院森林生态环境与保护研究 990613658@qq.com 
淮稳霞 中国林业科学研究院森林生态环境与保护研究  
林若竹 中国林业科学研究院森林生态环境与保护研究  
姚艳霞 中国林业科学研究院森林生态环境与保护研究  
赵文霞 中国林业科学研究院森林生态环境与保护研究  zhaowenxia@caf.ac.cn 
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中文摘要:
      [目的]鉴定引起新疆巩留县杏树果实斑点病病原菌。[方法]将巩留县杏树上采集到罹病杏采用常规组织分离法分离得到真菌菌株,然后利用传统形态学观察和分子系统学分析相结合的方式对所分离出菌株进行分类鉴定及其致病性检测。[结果]杏果病斑处的病原菌在显微镜下观察到分生孢子形态与经PDA培养基培养后观察到分生孢子形态均与Thyrostroma carpophilum (Lév.) B. Sutton所产生分生孢子一致;将分离获得的3株真菌的rDNA-ITS片段测序后与NCBI参考序列进行多重序列比对的结果显示,其序列与T. carpophilum一致性为100%;在基于ITS基因序列构建的系统发育树中,3株菌与T. carpophilum聚在同一分支。在接种了T. carpophilum后,杏果实和叶片均产生明显病斑并且从其所产生的病斑上再次分离到所接菌,满足柯赫氏法则。[结论]从新疆巩留县杏果实病斑处分离获得的3株真菌,经鉴定为引起杏穿孔病的病原菌T. carpophilum。这是该菌首次在该地区发现并报道。
英文摘要:
      [Objective] This study aims to identify the pathogen of apricot fruit spots disease in Gongliu County, Xinjiang. [Method] The pathogens were isolated by the conventional tissue separation method from the apricot trees of Gongliu. And the isolates were identified by morphological observation, phylogenetic analysis and pathogenicity. [Result] The conidia developed on apricot fruit lesions and PDA medium were consistent with the conidia produced by Thyrostroma carpophilum (Lév.) B. Sutton. Furthermore, the 3 strains were 100% identical to T. carpophilum by the sequence of rDNA-ITS and comparison with the NCBI reference sequence, and the 3 trains were homologous to T. carpophilum branch. After inoculation of T. carpophilum, both apricot fruits and leaves produced obvious lesions, and the pathogen were again isolated from the lesions produced by them, satisfying Koch's law. [Conclusion] The three strains isolated from the apricot fruit spots of Gongliu are T. carpophilum which is the first time that it was discovered and reported in the area.
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