园林废弃物木质素降解真菌的筛选、鉴别及其能力研究
Study on Screening, Identification and Their Capability of Lignin-Degrading Fungi for Landscaping Waste
投稿时间:2018-09-26  修订日期:2019-01-07
DOI:
中文关键词:  园林废弃物  木质素降解真菌  酶活力  固态发酵
英文关键词:landscaping waste  lignin-degradation fungi  enzyme activity  solid-state fermentation
基金项目:林业公益性行业科研专项(201504205)
作者单位E-mail
康跃 北京林业大学 ky637@bjfu.edu.cn 
李素艳 北京林业大学 lisuyan@bjfu.edu.cn 
孙向阳 北京林业大学  
龚小强 北京林业大学  
余克非 北京林业大学  
蔡琳琳 北京林业大学  
王琳 北京林业大学  
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中文摘要:
      为获得能够高效降解园林废弃物的真菌并研究其降解能力,从经过堆肥处理的园林废弃物中分离和筛选出1株木质素降解能力较强的真菌,经过形态分析、ITS分子序列分析,构建系统发育树,鉴定出该株木质素降解真菌种属,并利用该菌株进行固态发酵实验,研究其在固态发酵过程中漆酶(Lac)、锰过氧化物酶(MnP)的酶活力变化及其对不同园林废弃物中木质素的降解能力。从经过堆肥处理的园林废弃物中共挑选出真菌18株,筛选出1株高效的木质素降解真菌,编号为菌株No.11,经鉴定该菌株为构巢曲霉(Aspergillus nidulans)。以冬青卫矛叶、冬青卫矛枝、圆柏叶、圆柏枝、连翘为底物的模拟固态发酵的漆酶和锰过氧化物酶的酶活力峰值分别为388.6 U?L-1和138.3 U?L-1、326.2 U?L-1和121.1 U?L-1、461.3 U?L-1和104.5 U?L-1、342.7 U?L-1和128.6 U?L-1、588.4 U?L-1和73.3 U?L-1。添加菌株No.11使冬青卫矛叶、冬青卫矛枝、圆柏叶、圆柏枝、连翘40天后木质素降解率分别提高了20.40%、22.44%、29.90%、25.28%、15.77%。筛选出构巢曲霉对北京地区常见园林废弃物冬青卫矛枝叶、圆柏枝叶和连翘中木质素具有较好的降解能力,在降解园林废弃物方面可能更具有优势。
英文摘要:
      To obtain a fungus that can efficiently degrade landscaping waste, and to study its ability to degrade different kinds of landscaping waste. The fungus strain was selected from the composted landscaping waste by a series of isolation and culture. By morphological analysis, ITS molecular sequence analysis, built phylogenetic tree, identified its species of the lignin decomposition strain. And by solid-state fermentation experiments, studied changes of enzyme activity of laccase (Lac) and manganese peroxidase (MnP) of the strain and their ability to decompose lignin in landscaping waste. A total of 18 fungi were selected from the composted landscaping waste and a highly efficient lignin-decomposing fungus was selected. The strain was designated as strain No.11, and the strain was identified as Aspergillus nidulans by morphological analysis and ITS molecular sequence analysis. The enzyme activity peaks of laccase and manganese peroxidase in solid canisters with Japanese Spindle leaves, Japanese Spindle branches, Juniper leaves, Juniper branches and forsythia as the substrates were 388.6 U?L-1 and 138.3 U?L-1, respectively. ? L-1, 326.2 U?L-1 and 121.1 U?L-1, 461.3 U?L-1 and 104.5 U?L-1, 342.7 U?L-1 and 128.6 U?L-1, 588.4 U? L-1 and 73.3 U?L-1. The addition of strain 11 increased the lignin decomposition rate of Japanese Spindle leaves, Japanese Spindle branches, Juniper leaves, Juniper branches and forsythia for 20 days, respectively, by 20.40%, 22.44%, 29.90%, 25.28%, and 15.77%. The lignin-decomposing fungus Aspergillus nidulans has a good ability to decompose the lignin in the branches and leaves of the Japanese Spindle, Juniper and forsythia common to the landscaping waste in Beijing region. Aspergillus nidulans may have advantages in decomposing landscaping waste.
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