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新疆野苹果林中疫霉菌种类鉴定及致病性研究

程元 赵文霞 哈米提 林若竹 姚艳霞 薛寒 淮稳霞

引用本文:
Citation:

新疆野苹果林中疫霉菌种类鉴定及致病性研究

    通讯作者: 淮稳霞, huaiwx@caf.ac.cn
  • 基金项目:

    国家重点研发计划课题 2016YFC0501503

    国家自然科学基金项目 31470649

    林业微生物资源共享服务子平台运行与服务支撑 NIMR-2019-7

  • 中图分类号: S763.1

Identification and Pathogenicity of Phytophthora Species from Wild Apple Forests in Xinjiang, China

    Corresponding author: Wen-xia HUAI, huaiwx@caf.ac.cn
  • CLC number: S763.1

  • 摘要: 目的 通过研究新源县和巩留县野苹果林中疫霉菌的种类和致病性差异,为探讨新疆野苹果林衰亡原因提供基本资料,为野苹果林病害防治提供科学依据。 方法 采用疑似疫霉病害样品采集、土壤诱捕、林间溪流诱捕等方法对新疆野苹果林中的疫霉菌进行了调查采样和监测诱捕,并利用选择性培养基分离获得疫霉纯菌株。通过形态学观察和rDNA-ITS序列分析相结合的方法对所获得的疫霉菌株进行分类鉴定,并通过离体叶片接种试验对所鉴定的疫霉菌进行致病性测定。 结果 依据形态学特征和序列分析,鉴定得到5个种共计88株疫霉,分别为65株湖沼疫霉Phytophthora lacustris Brasier,Cacciola,Nechwatal,Jung & Bakonyi、16株节水霉状疫霉Phytophthora gonapodyides(Petersen)Buisman、3株多寄主疫霉Phytophthora plurivora T. Jung & T. I. Burgess、2株聚疫霉Phytophthora gregata T. Jung,M. J. C. Stukely & T. I. Burgess和2株Phytophthora sp. 1。将前4种疫霉接种离体野苹果叶片后,发现它们均产生病斑,其中湖沼疫霉在离体叶片上产生的病症最为明显。 结论 新疆野苹果林中具有多种疫霉菌,且所鉴定的4种疫霉菌均对野苹果叶片有一定的致病性。
  • 图 1  基于最大似然法构建的疫霉菌clade 6系统发育树

    Figure 1.  Maximum likelihood phylogenetic tree established for Phytophthora species in clade 6

    图 2  基于最大似然法构建的疫霉菌clade 2系统发育树

    Figure 2.  Maximum likelihood phylogenetic tree established for Phytophthora species in clade 2

    图 3  4种疫霉菌在胡萝卜琼脂培养基上生长的菌落形态

    Figure 3.  Colony morphology of four isolates of Phytophthora specieson Carrot Agarmedia

    图 4  4种疫霉菌形态结构特征

    Figure 4.  Morphological structures of four strains of Phytophthora

    图 5  4种疫霉菌接种野苹果离体叶片发病情况

    Figure 5.  Symptoms on detached leaves of wild apples after inoculation with four isolates of Phytophthora species

    图 6  4种疫霉侵染离体野苹果叶片7 d后所产生病斑的平均大小

    Figure 6.  The average size of lesions on infected leaves of wild apples 7 d after inoculation with four isolates of Phytophthora species

    表 1  野苹果林疫霉菌分布信息表

    Table 1.  Distribution of Phytophthora species in wild apple forests

    种类
    Species
    时间
    Time
    地点
    Site
    方法
    Method
    数量
    Quantity
    湖沼疫霉
    P. lacustris
    2016 巩留 土壤诱捕 2
    2017 新源 溪流诱捕 23
    巩留 溪流诱捕 40
    节水霉状疫霉
    P. gonapodyides
    2016 新源 土壤诱捕 3
    2017 新源 溪流诱捕 3
    巩留 溪流诱捕 10
    多寄主疫霉
    P. plurivora
    2016 新源 土壤诱捕 1
    2017 新源 土壤诱捕 2
    聚疫霉P. gregata 2016 巩留 溪流诱捕 2
    Phytophthora sp. 2016 新源 溪流诱捕 1
    巩留 溪流诱捕 1
    下载: 导出CSV
  • [1]

    Cavalier-Smith T. Only six kingdoms of life[J]. Proceedings of the Royal Society B, 2004, 271:1251-1262. doi: 10.1098/rspb.2004.2705
    [2] 郑小波.疫霉菌及其研究技术[M].北京:中国农业出版社, 1997.

    [3]

    Erwin D C, Ribeiro O K. Phytophthora diseases worldwide[J]. St. Paul, MN:American Phytopathological Society, 1996, 90(6):1092.
    [4]

    Brar S, Tabima J F, Mc Dougal R L, et al. Genetic diversity of Phytophthora pluvialis, a pathogen of conifers, in New Zealand and the west coast of the United States of America[J]. Plant Pathology, 2018, 67(5):1131-1139. doi: 10.1111/ppa.12812
    [5]

    Goheen E M, Hansen E M, Kanaskie A, et al. Sudden oak death caused by Phytophthorara morum in Oregon[J]. Plant Disease, 2007, 68(4):441-441.
    [6]

    Rizzo D M, Garbelotto M, Hansen E M. Phytophthorara morum:integrative research and management of an emerging pathogen in California and Oregon forests[J]. Annual Review of Phytopathology, 2005, 43(1):309-335. doi: 10.1146/annurev.phyto.42.040803.140418
    [7]

    Rizzo D M, Garbelotto M, Davidson J M, et al. Phytophthorara morum as the cause of extensive mortality of Quercus spp. and Lithocarpus densiflorus in California[J]. Plant Disease, 2007, 86(3):205-214.
    [8]

    Reeser P, Sutton W, Hansen E. Phytophthora species in tanoak trees, canopy-drip, soil, and streams in the sudden oak death epidemic area of south-western Oregon, USA[J]. New Zealand Journal of Forestry Science, 2011, 41S:S65-S73.
    [9]

    Jung T, Burgess T I. Re-evaluation of Phytophthora citricola isolates from multiple woody hosts in Europe and North America reveals a new species Phytophthora plurivora sp. nov.[J]. Persoonia, 2009, 22(5):95-110.
    [10] 林培钧, 崔乃然.天山野果林资源-伊犁野果林综合研究[M].北京:中国林业出版社, 2000.

    [11] 于少帅, 赵文霞, 姚艳霞, 等.新疆野苹果枯枝症状级别与水杨酸含量、胸径关系研究[J].林业科学研究, 2019, 32(2):111-116.

    [12] 阎国荣, 许正.天山野生果树主要病害及其分布[J].干旱区研究, 2001, 18(2):47-49.

    [13] 刘爱华, 张新平, 温俊宝, 等.天山野苹果林苹果小吉丁与苹果腐烂病复合危害研究[J].新疆农业科学, 2014, 51(12):2240-2244.

    [14] 牛程旺, 王静茹, 朱小琼, 等.新疆野果林褐腐病菌的种类[J].菌物学报, 2016, 35(12):1514-1525.

    [15] 王智勇, 张彦龙, 杨忠岐, 等.苹小吉丁(鞘翅目:吉丁甲科)幼虫龄数的测定[J].林业科学研究, 2013, 26(6):786-789.

    [16] 孔婷婷, 刘爱华, 岳朝阳, 等.天山野苹果林苹果小吉丁虫生真菌调查[J].北方园艺, 2017(1):138-141.

    [17] 薛煜, 刘雪峰, 项存悌.林病研究方法[M].哈尔滨:东北林业大学出版社, 2013.

    [18]

    Nechwatal J, Bakonyi J, Cacciola S O, et al. The morphology, behaviour and molecular phylogeny of Phytophthora taxon Salixsoil and its redesignation as Phytophthora lacustris sp. nov.[J]. Plant Pathology, 2012, 62(2):355-369.
    [19]

    Brasier C M, Hamm P B, Hansen E M. Cultural characters, protein patterns and unusual mating behaviour of Phytophthora gonapodyides isolates from Britain and North America[J]. Mycological Research, 1993, 97(11):1287-1298. doi: 10.1016/S0953-7562(09)80160-3
    [20]

    Jung T, Stukely M J, Hardy G E, et al. Multiple new Phytophthora species from its clade 6 associated with natural ecosystems in Australia:evolutionary and ecological implications[J]. Persoonia, 2011, 26(1):13-39. doi: 10.3767/003158511X557577
    [21] 淮稳霞.中国西南地区杜鹃-栎树林中疫霉菌的分离鉴定及快速检测技术研究[D].北京, 中国林业科学研究院博士学位论文, 2013.

    [22]

    White T J, Bruns T D, Lee S B, et al. Amplification and direct sequencing of fungal ribosomal RNA Genes for phylogenetics//PCR-Protocols and Applications-A Laboratory Manual[M]. San Diego, CA: Academic Press, 1990.
    [23]

    Altschul S F. Basic local alignment search tool[J]. Journal of Molecular Biology, 1990, 215(3):403-410.
    [24]

    Katoh K, Rozewicki J, Yamada K D. MAFFT online service:multiple sequence alignment, interactive sequence choice and visualization[J]. Briefings in Bioinformatics, 2017, (4):1-7.
    [25]

    Tamura K, Peterson D, Peterson N, et al. MEGA5:molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods[J]. Molecular Biology and Evolution, 2011, 28(10):2731-2739. doi: 10.1093/molbev/msr121
    [26]

    Maddison W P, Maddison D R. Mesquite: a modular system for evolutionary analysis[CP]. Version 3.5.1, 2018. http://www.mesquiteproject.org.
    [27]

    De D I, Vercauteren A, Speybroeck N, et al. Effect of host factors on the susceptibility of Rhododendron to Phytophthorara morum[J]. Plant Pathology, 2010, 59(2):301-312. doi: 10.1111/j.1365-3059.2009.02212.x
    [28]

    Huai W X, Tian G, Hansen E M, et al. Identification of Phytophthora species baited and isolated from soil and streams in northwestern Yunnan province, China[J]. Forest Pathology, 2013, 43(2):87-103. doi: 10.1111/efp.12015
    [29] 李雯雯.中国典型生境中森林疫霉的调查和分类研究[D].北京, 中国林业科学研究院硕士学位论文, 2018.

    [30]

    Balci Y, Halmschlager E. Incidence of Phytophthora species in oak forests in Austria and their possible involvement in oak decline[J]. Forest Pathology, 2003, 33(3):157-174. doi: 10.1046/j.1439-0329.2003.00318.x
    [31]

    Cooke D E L, Drenth A, Duncan J M, et al. A molecular phylogeny of Phytophthora and related oomycetes[J]. Fungal Genetics and Biology, 2000, 30(1):17-32. doi: 10.1006/fgbi.2000.1202
    [32]

    Blair J E, Coffey M D, Park S Y, et al. A multi-locus phylogeny for Phytophthora utilizing markers derived from complete genome sequences[J]. Fungal Genetics and Biology, 2008, 45(3):266-277. doi: 10.1016/j.fgb.2007.10.010
    [33]

    Martin F N, Blair J E, Coffey M D. A combined mitochondrial and nuclear multilocus phylogeny of the genus Phytophthora[J]. Fungal Genetics and Biology, 2014, 66(3):19-32.
    [34]

    Yang X, Tyler B M, Hong C. An expanded phylogeny for the genus Phytophthora[J]. IMA Fungus, 2017, 8(2):355-384. doi: 10.5598/imafungus.2017.08.02.09
    [35]

    Liu A H, Shang J, Zhang J W, et al. Canker and fine-root loss of Malus sieversii(Ldb.)Roem. caused by Phytophthora plurivora in Xinjiang Province in China[J]. Forest Pathology, 2018, e12462. https://doi.org/10.1111/efp.1246
    [36]

    Burgess T I, Simamora A V, White D, et al. New species from Phytophthora Clade 6a:evidence for recent radiation[J]. Persoonia, 2018, 41:1-17. doi: 10.3767/persoonia.2018.41.01
    [37]

    Brasier C M, Cooke D E L, Duncan J M, et al. Multiple new phenotypic taxa from trees and riparian ecosystems in Phytophthoragona podyides-P. megasperma ITS Clade 6, which tend to be high-temperature tolerant and either inbreeding or sterile[J]. Mycological Research, 2003, 107(3):277-290. doi: 10.1017/S095375620300738X
    [38]

    Reeser P W, Sutton W, Hansen E M, et al. Phytophthora species in forest streams in Oregon and Alaska[J]. Mycologia, 2011, 103(1):22-35. doi: 10.3852/10-013
    [39]

    Cacciola S O, Williams N A, Cooke D E L, et al. Molecular identification and detection of Phytophthora species on some important Mediterranean plants including sweet chestnut[J]. Forest Snow and Landscape Research, 2001, 76(3):351-356.
    [40]

    Orlikowski L B, Ptaszek M, Rodziewicz A, et al. Phytophthora root and collar rot of mature fraxinus excelsior in forest stands in Poland and Denmark[J]. Forest Pathology, 2011, 41(6):510-519. doi: 10.1111/j.1439-0329.2011.00714.x
    [41]

    Nechwatal J, Mendgen K. Widespread detection of Phytophthora taxon Salixsoil in the littoral zone of lake constance, Germany[J]. European Journal of Plant Pathology, 2006, 114(3):261-264. doi: 10.1007/s10658-005-5593-y
    [42]

    Jung T, Nechwatal J. Phytophthora gallica sp. nov. a new species from rhizosphere soil of declining oak and reed stands in France and Germany[J]. Mycological Research, 2008, 112(10):1195-1205. doi: 10.1016/j.mycres.2008.04.007
    [43]

    Corcobado T, Cubera E, Pérezsierra A, et al. First report of Phytophthora gonapodyides involved in the decline of Quercus ilex in xeric conditions in Spain[J]. New Disease Report, 2010, 22:33. doi: 10.5197/j.2044-0588.2010.022.033
    [44]

    Robideau G P, Cock D A W A M, Coffey M D, et al. DNA barcoding of oomycetes with cytochrome c oxidase subunit I and internal transcribed spacer[J]. Molecular Ecology Resources, 2011, 11(6):1002-1011. doi: 10.1111/j.1755-0998.2011.03041.x
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  • 收稿日期:  2019-01-21
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  • 刊出日期:  2019-12-01

新疆野苹果林中疫霉菌种类鉴定及致病性研究

    通讯作者: 淮稳霞, huaiwx@caf.ac.cn
  • 1. 中国林业科学研究院森林生态环境与保护研究所, 国家林草局森林保护学重点实验室, 北京 100091
  • 2. 新疆额敏县自然资源局, 新疆 额敏 834600
基金项目:  国家重点研发计划课题 2016YFC0501503国家自然科学基金项目 31470649林业微生物资源共享服务子平台运行与服务支撑 NIMR-2019-7

摘要:  目的 通过研究新源县和巩留县野苹果林中疫霉菌的种类和致病性差异,为