• 中国中文核心期刊
  • 中国科学引文数据库(CSCD)核心库来源期刊
  • 中国科技论文统计源期刊(CJCR)
  • 第二届国家期刊奖提名奖

2005 Vol. 18, No. 3

Display Method:
Construction of Co-expressing Vector for Gly Bet Synthesis and Salt Tolerance
GAO Zhi-min, PENG Zhen-hua
2005, 18(3): 231-235.
Abstract:
Glycine betaine (GlyBet) is an established target for metabolic engineering of stress tolerance because it is a potent osmoprotectant that many plants lack. GlyBet is synthesized in the chloroplast by a two-step oxidation of choline (Cho) that is catalyzed by CMO and BADH. Cho is one of essential precursors for the synthesis of GlyBet. PEAMT is a key enzyme in the Cho biosynthetic pathway and serves as a step-limiting controller. The CMO cDNA and BADH cDNA drived by 35S and the PEAMT genomic DNA were cloned into the binary vector(pCAMBIA1300) containing HPTⅡgene step by step. The plasmid was checked by 7 enzymes, and the fingerprints were expected. The plasmid of this expressing vector was transferred into the Agrobacterium tumefaciens (GV3101) by electroporation, then used in genetic transformation of A. thaliana mediated by vacuum infiltration. The primary transgenic plants were selected for hygemycin resistance and verified by PCR. The transgenic lines showed less growth inhibitation than control on 1/2MS containing 125 mmol·L-1 NaCl.
Effect of 50% CO2 and 100% N2 Shock Treatment on the Quality Changes of Chinese Chestnut (Castanea mollissima Blume) during Shelf-life
LIANG Li-song, WANG Gui-xi
2005, 18(3): 236-240.
Abstract:
Chinese chestnut (Castanea mollissima Blume, cv. Yanchang) stored 180 days at 0℃ were treated with 50%CO2 or 100%N2 for 5days or 10days to investigate the quality changes of chestnut during shelf-life. The result indicated that compared with other treatments, 50%CO2 shock treatment decreased the sprouting rate and rot rate of chestnut significantly, and maintaining quality during shelf-life. High N2 treatment increased the sprouting rate of chestnut, and can not contorl chestnut decay effectively during shelf-life. Chestnut treated with 50%CO2 or 100%N2 showed higher content of starch and lower content of soluble suger than control,but there was no significant effect on proten content.Chestnut with shock treatment in 50% CO2 for 5 days had the better quality during shelf-life.
Isolation and Culture of Parthenocissus tricuspidata and P.quenquefolia Protoplasts
LI Zheng-hong, SUN Zhen-yuan, LIU Xiu-xian, PENG Zhen-hua
2005, 18(3): 241-245.
Abstract:
Mixed enzyme liquid of cellulase R-10,Pectolyase Y23and mannitol was used to extract the protoplasts from sterilized seedling leaves and endosperm callus of Parthenocissus tricuspidata and P.quenquefolia.The factors affectiong the yield and activities of portoplasts were analyzed and the yield of protoplast from different sources was compared.The protoplasts obtained were cultured by liquid,solid-liquid and solid culture methods.The differentiated cultures were conducted on the callus formed from protoplasts on MS and modified B5 media with different concentrations of NAA,6-BA,2,4-D,PEG,KT,and ZT.The results showed that cellulase R-10 had extremely singificant influences on the extract yield of protoplasts.The suitable enzyme liquid combination for extracting protoplast of P.tricuspidata was:cellulase R-10 0.3%,mannitol 0.6 mol·L-1,with enzymolysis time 8 hours.There existed significant differences among different sources in the yield of protoplast.Only the protoplast from P.quenquefolia endosperm callus could form new callus on soild media.While the protoplasts from other sources had no divistion or disintegrated after forming cell groups containing dozens of cells.The new callus tissues formed from endosperm callus protoplasts of P.quenquefolia were differentiated cultured with over thirty prescriptions,and no differentiation was found after 6-10 subculture.
A Survey on Heavy-metal Content in Plants and Soils in Zinc-lead Mine Tailing Wasteland
LIU Cheng-zhi, SHANG He, YAO Bin, YU Cheng-zhi, LIANG Jing-sen, HU Xi-you
2005, 18(3): 246-249.
Abstract:
A survey on soils and nine plants growing on the soil polluted by Zinc-lead mine tailing wasteland in Tieling City was conducted. The concentration and heavy-metal enrichmentalabilities of Cu, Cd, Zn and Pb in the nine plants were measured. Populus simonii and Rosa davurica were proved to have certainly Zn-tolerant ability. The concentration of Cd in Populus simonii and Artemisia sacrorum were higher than that of other species. While Pb were higher in Spiraea blumei and Scabicsa tschiliensis. However, no hyperaccummulators of these four heavy metals were found in the survey. Further research on how to remediate the zinc-lead contaminated soil by integrated methods should be conducted.
Study on Fiber Forms and Tissue Measurements of Bambusa wenchouensis Wood
SU Wen-hui, GU Xiao-ping, MA Ling-fei, WU Xiao-li, YUE Jin-jun
2005, 18(3): 250-254.
Abstract:
The fiber forms and tissue measurements of Bambusa wenchouensis wood in the southern area in Zhejiang province were tested and compared with those of B. textilis and Dendrocal_amopsis oldhami. The analytic results showed that the fiber length (L) of B. wenchouensis was 2.24 mm, a little shorter than that of B. textilis (2.29 mm)but longer than D. oldhami(1.87 mm); the ratio of fiber length to width (L/W) was 166, the largest one among the three woods, and those of the other bamboospe cies were 147 and 131 respectively; the ratio of wall thickness of fiber cell to the cavity diameter (T/D) was 3.5; measurements of fiber tissue was 48.68%, a bit smaller than that of B. textilis (50.19%)but larger than D. oldhami(45.78%). The fiber forms and tissue measurements were variable in both axial and radial parts of this bamboo: T/D and fiber tissue measurements increased gradually from the culm foot to tip; the fiber length and L/W both were the largest in the central bamboo wall, while T/D was the highest in the outer wall. The age had a significant influence on T/D, which was much larger of 1 or 3-year-old wood than that of month-old wood.
Studies on Fertilization of Young Acer truncatum Bunge Ⅱ. Effect on Flavonoid,Cholorogenic Acid and Nutrition Elements in Leaves
SU Jian-rong, DENG Jang, LUO Xiang, YANG Wen-yun
2005, 18(3): 255-259.
Abstract:
By determining the content of flavonoid,cholorogenic acid and ten nutrition elements in the leaves of Acer truncatum treated by 9 kinds of nutrition supplying, by analyzing the principal components, the dynamic relationship among nutrition elements and the content of flavonoid and cholorogenic acid were investigated. The result indicated that the content of flavonoid and cholorogenic acid in leaves of the two best fertilization treatments was 100%NFA48150% higher than the control. The effect of treatment on the content and the accumulation content of nutrition elements was similar, but the effects on flavonoid and cholorogenic acid was negatively correlated. Of the five principal factors affecting the content of flavonoid and cholorogenic acid, two were found to be principal: factors of promoting photosynthesis and nitrogen, and factors of promoting enzyme react. The accumulated variation cortribution rate of the two factors was more than 87% and the loading of P, Cu and Mg was the biggest among the loading matrix of principal components.
Analysis of Platycladus orientalis Volatiles and Their Electroantennogram Responses with Semanotus bifasciatus
KONG Xiang-bo, ZHANG Zhen, WANG Hong-bin, YANG Jie, HU Yu-tian
2005, 18(3): 260-266.
Abstract:
Behavioral tests revealed that Semanotus bifasciatus was easy to be attracted to its host trees species, Platycladus orientalis. The volatiles emitted from different parts of P. orientalis were collected outdoors by headspace sampling in situ and analyzed by Thermodesorption Cold Trap (TCT)-GC-MS. Two major classes of compounds, aliphatics [mainly green-leaf volatiles (GLVs)] and monoterpenes, existed in the host tree species investigated. Sesquiterpenes and monoterpenes (and their derivatives) sampled from the phloem by the solid phase microextraction (SPME) and hydrodistillation made up the major part of whole volatile blends. However, differences could be found with respect to a few components of the blend. Electroantennograms from the antennae of S. bifasciatus were recorded in response to compounds presented in the headspace of P. orientalis and other sources. The antennae responded strongly to green leaf alcohols: 1-Pentanol, Hexanol, 2-ethyL-1-Hexanol and 1-Octen-3-ol; but a little EAG amplitudes were observed to aldehydes; Better antennal responses to Sabinene, Thujanol and (一)-Limonene were found. In addition, the antennae were more sensitive to that of bark beetle pheromones than some monoterpenes and alcohols, especially the compounds 3-methyl-2-cyclohexen-1-ol and 3-methyl-2-cyclohexen-1-one. The blends of C6 and C8 alcohols plus verbenone together with monoterpenes might play an important role in host selection of S. bifasciatus.
A Study on Ecological Characteristics of Suillus luteus(L.:Fr)Gray
YING Guo-hua, LU Ming-liang, CHEN Yi-liang, CHEN Lian-qing, MEI Jian-wei, JIA Ya-ni, YAO Yue-hua
2005, 18(3): 267-273.
Abstract:
The excellent edible fungus Suillus luteus appeared in winter in the low altitude in the masson pine stand. Through 3 years of investigation and observation,the morphological character, the embryology and development of fruit bodies,the forest stand structure where the fruit bodies occurred and the weather factors were studied. It revealed the relationship between the growth of Suillus luteus fruit bodies and the weather,plant and soil.It pointed out the main factors determining the growth of fruit bodies of the edible fungus Suillus luteus and the greatest yield were accompanying tree species,site,forest stand structure and soil;while the rainfall and temperature were the key factors affecting the occulting time and yield of fruit bodies in the current year.
Studies on the Hydrologic Characteristics of Litter under Phyllostachys pubescens in Several Different Forest Stands
GAO Zhi-qin, FU Mao-yi
2005, 18(3): 274-279.
Abstract:
This paper dealt with a study on comparisng the hydrologic characteristics of litter layers under different structure of Phyllostachys pubescens and evergreen broadleaved forest stands. It showed that the water absorptive process of litter under various stands can be obviously divided into two phases in 24 hours as follow.
Two Newly Described Polypores on Populus in Northeast China
JIANG Jun-qing, YUAN Hai-sheng
2005, 18(3): 280-283.
Abstract:
Two newly described polypores, Antrodia leucaena and Spongipellis sibirica (Penzina Ryvarden) Penzina Kotir., were reported from Northeast China. Both species are growth species of Populus. An illustrated description of the two polypores was given in detail based on our the specimen from China. Antrodia leucaena is characterized by resupinate to effusedreflexed basidiocarps, a dimitic hyphal structure with clamp connections on generative hyphae, cylindric basidiospores, and by causing a brown rot. It is found only in northeast China so far. Antrodia leucaena is similar to Antrodia pulvinasce and Antrodia macra (Sommerf.) Niemel, the latter two species occur on Populus as well. However, both species produce resupinate basidiocarps, and neither cystidioles nor crystals present in both species. In addition, A. pulvinascens has ellipsoid basidiospores, and Antrodia macra has large pores and,big basidiospores. Spongipellis sibirica is characterized by pileate, soft and watery basidiocarps, a monomitic hyphal structure with clamp connections on generative hyphae, broad ellipsoid, and by causing a white rot. It is growth on living trees of poplar, and is found in northeast China and Siberia of Russia. Spongipellis sibirica resembles Spongipellis spumea (Sowerby:Fr.) Pat., but the latter species has distinctly big basidiospores.
Design of Information System for Forest Resource Management on County Level
HONG Ling-xia, LU Yuan-chang, LEI Xiang-dong, XIE Yang-sheng, GUO Hong, MU Hui-sheng
2005, 18(3): 284-291.
Abstract:
Design for Forest Resource Management Information System on County Level was described, including applying "the standards and criteria of digital forestry" in the information system, using software of logical model designer and Standard Modelling Language for visual logical models and its guiding principles for application. The detailed contents were: (1) guiding principles and methods for system development; (2) the goals and requirements of system design; (3) system analysis and designs including user demands; general system logical model; table of system database; classification and the denominate rule of attribute data; regulation for organizing and managing of geographic data, and design of system functions; (4) developing process and method of the Information System for Forest Resource Management on County Level.
Physiological Changes of `Dongzao’ Jujube during the Controlled Atmosphere Storage
ZONG Yi-chen, WANG Gui-xi, FENG Shuang-qing
2005, 18(3): 292-295.
Abstract:
The changes in physiological characteristics of fresh jujube (Zizyphus jujuba Mill.cv. ‘Dongzao’) stored in controlled atmosphere(CA) and in bored film package(CK) were investigated. The results showed that the total phenols content of jujube stored in CA (O2 12%NFA4815%, and no CO2) was much more than that of the CK. There was a significant negative correlation between the total phenols content and membrane permeability in fruits (rCA=-0.99). CA treatment showed a better benefit of inhibiting the activities of the polyphenol oxidase (PPO) and peroxidase (POD), delaying the peak time of the PPO compared to CK. Meanwhile, the results showed that the membrane permeability of jujube was kept in a integrate state by CA, and the increase of alcohol content was also slowed down by CA. The browning and ferment of jujube could be inhibited by CA. And the jujube life was prolonged.
Study on Geographical Provenance Variations of Dalbergia obtusifolia Prain
SUN Yong-yu, LI Kun, YANG Wen-yun, LI Li
2005, 18(3): 296-299.
Abstract:
Through the study on the indexes of color, length and width of the legumens,seeds weight, germination potential, germination ratio, seedlings height of Dalbergia obtusifolia of different geographical provenances, the result showed that the above indexes was complicated and variational.Even in a same geographical provenance, different family’s indexes were also different. The relation between provenance temperature and the indexes of weight,width of the legumens and seeds number of legumens were close.
Relationship between Lipoxygenase and ABA and JA in Wounded Signal Transduction of Heathy Populus Seedlings
ZHANG Ke-wen, AN Yu, HU Zeng-hui, YANG Di, SHEN Ying-bai
2005, 18(3): 300-304.
Abstract:
Using the poplar as materials, the authors examined and compared the activity of lipoxygenase (Lox) and the dynamic level of ABA and JA in different leaves: the mechanical damaged leaves and the leaves of the neighboring healthy plant after being damaged. The result showed that: (1) the mechanical wounding enhanced the activity of Lox and induced the concentration of ABA and JA increasing in the leaves. It could be concluded that mechanical wounding could induce the defensive of the damaged plant. Furthermore, there might exist some airborne signals, which transferred the wounding message to neighboring plants, and induced defense reaction of the neighboring healthy plants. (2) Lox activity and the contents of ABA and JA increased with similar trend along with the mechanical wounding, indicating that Lox maybe act to the key process of the biosythesis to ABA and JA.
The Measurement of Ecological Sustainability on Community Forest Management
JIANG Chun-qian, YU Shu-quan, ZHANG Shou-gong, ZHU Zhao-hua, LIU Wei
2005, 18(3): 305-309.
Abstract:
Based on the participatory approach, the criteria and indicators on the measurement of the ecological sustainability of community forest management were built in 9 villages of 3 townships in Lin’an, Zhejiang of China. Combined with experts’ methods, the weights, thresholds and score methods of indicators were also developed and the ecological sustainability on forest management was measured under the hierarchical structure in 3 townships. The assessment system was composed of 11 indicators belonging to the criteria of the conservation of biodiversity, maintenance of forest productivity, maintenance of forest health, and environment response on the forest management. The verifiers under the indicators were established so as to collect and quantify the data. The assessment results showed that ecological sustainability scores in the townships of Changhua, Gaohong and Lin’mu in 1994 and 2002 were 5.65, 5.64, 4.68 and 5.09, 5.47, 6.88 respectively, which increased by -0.18%, 8.76% and 25.78% respectively. Compared with the other 2 townships, the ecological sustainability in Lin’an has increased a lot in the past decade. The score on ecological sustainability in 3 townships in 1994 and 2002 was higher than 5 which was defined as good forest management before testing, which showed the forest management in Lin’an is in the state of good condition, but there is a long to get the ideal condition towards sustainable forest management.
Path Analysis on the Crown Characteristics and Growth of Manglietia yuyuanensis in Mixed Stands
LI Sheng, CHEN Cun-ji
2005, 18(3): 310-314.
Abstract:
The correlation analysis on crown characterstics and individual volume growth of Manglietia yuyuanansis grown in 17-year-old mixed stand (Cunninghamia lanceolata× Manglietia yuyuanensis) was conducted. The results showed that the correlation coefficients betwwen individual volume index (D2H) and crown factors were different. Except single leaf area index (LAI) and crown layer density(CLD), the total leaf area of single tree (TLA),the tree crown area (TCA) and crown shape rate (CSR) were positively correlated to the individual volume index. The path analysis showed that TCA had the most direct effect on individual volume growth(Pyxi=1.201 3), which was the key factor affecting the individual volume growth of M.yuyuanensis, while TLA, CLD, CSR played their roles through TCA. The importance of the five crown factors above-mentioned on the individual volume growth of M.Yuyuanensis was in the order of TCALAIYLACSRCLD. It was found during analyzing the influence of crown spatial structure on individual volume growth that in vertical direction, the leaf area in middle and lower layers played decisive role to individual volume growth and the upper layer played less role:while in horizontal direction, the leaf area in the inner and outer parts contributed more to the individual volume growth and that in the middle part contributed less.
Study on the Effect of Different Cistanche deserticola Excavation Hole Treatments on Soil Moisture Content around Haloxylon ammodendron Rhizosphere
GUO Quan-shui, TAN De-yuan, WANG Chun-ling, SHI Zuo-min, MA Chao
2005, 18(3): 315-320.
Abstract:
By selecting an experiment plot from the fine forest experiment station in Jimusaer County of Xinjiang Uygur Autonomous Region,where the site conditions,way of inoculation Cistanche deserticola age of host sapling,planting sapling density were well selected all analogous in the fine tree seeds production base of Cistanche deserticola,we have studied the effect of different excavation hole treatments on soil moisture content around Haloxylon ammodendron rhizosphere. The results showed that the excavation hole resulted from Cistanche deserticola excavation had significant effect on the soil moisture content, with an influence range differing in soil layers. At the level of 0NFA4820 cm surface soil, the effect was most significant. The soil closer to the excavation hole had less moisture content. In the soil layer of more than 20 cm below the ground, this changing trend still existed. However, locations of more than 45 cm apart from the excavation hole, the influence of excavation hole on soil moisture content became less. The soil moisture content of different Cistanche deserticola excavation hole treatments existed significant differences. In the soil layer of 0NFA4810 cm, the ranking of soil moisture content in descending order was as follows: the control group backfill excavation hole side inner backfill excavation hole non-backfill excavation hole side. In the soil layer of 10NFA4830 cm, the ranking of soil moisture content in descending order was as follows: backfill excavation hole side the control group inner backfill excavation hole non-backfill excavation hole side. In the soil layer of 30NFA4850 cm,the ranking of soil moisture content in descending order was as follows: the control group inner backfill excavation hole backfill excavation hole side non-backfill excavation hole side. The main reason for a low water moisture content in non-backfill excavation hole was that excavation increased the evaporation profile and exposure of deep layer soil to atmosphere, making a continuous evaporation of soil moisture content in the excavation hole sides. Backfill excavation hole could put off or prevent the pervasion of the moisture content from the excavation hole side to atmosphere. However, due to the fact that the backfill soil structure changed, therefore, the backfill soil moisture content changed as time changed, which was quite different from the changing rules in the control group, backfill excavation hole side and non-backfill excavation hole side.
Studies on the Karyotype and Chromosome Variation of Regenerated Perennial Ryegrass
FENG Xia, SUN Zhen-yuan, LIU Jian-feng, PENG Zhen-hua, DU Xiao-juan
2005, 18(3): 321-324.
Abstract:
The chromosome karyotypes and the chromosome variation of regenerated plants of perennial ryegrass(Lolium perenne L.)were studied. The results showed that perennial ryegrass was diploid and composed of metacentric chromosomes and submetacentric chromosomes and belonged to "1A" type. Its karyotypical formula was 2n=2x=14=2sm+12m(2SAT). It was also found that the chromosome amount changes happened in 40% of regenerated plants cells and there were also obvious chromosome structure variations (fragment,dicentrics,condensation and transposed ring etc.), mitotic aberrations (lagging chromosome, bridges) as well as numerical alterations (including haploid,aneuploid and polyploid etc.)in some cells of regenerated plants.
A Comparison on the Early Growth of Cutting Stock and Seedlings of Slash Pine × Caribbean Pine F1 Hybrids
ZHAO Fen-cheng, ZHANG Ying-zhong, LI Fu-ming, ZHONG Sui-ying, LIU Hong-jie, HUANG Yong-quan
2005, 18(3): 325-330.
Abstract:
The cutting stocks and seedlings of 7 full-sib families of slash pine (Pinus elliottii var. elliottii) × caribbean pine (P. caribaea var. hondurensis) F1 hybrid were planted in a trial in Taishan (22°11′N,112°49′E). The test was measured at age 1 for survival and height; and at age 3, 4 for height, diameter at breast height (DBH). The average survival rates of cutting stocks and seedling were 97.19% and 97.55%, respectively. All hybrid families performed better than seed-lot of slash pine first-generation seed orchard in Taishan, at the age of 4. Relative realized gains of cutting stocks and seedlings of the slash pine seed-lot were 29% and 26%, 34% and 34%, 133% and 135%, for height, DBH and volume, respectively. The total growth of cutting stocks was slower than that of seedlings, in the first 3 years. But the increment of cutting stocks increased gradually, and it exceeded the seedlings at the age of 4. It was suggested that the cutting stocks or seedlings of superior hybrid families could be planted extensively in the southern region of China.
A Study on the Stem Sap Flow of Acacia mangium
XIAO Yi-hua, CHEN Bu-feng, CHEN Jia-jie, CHEN Yong, LI Dong-wen, WU Tong-gui
2005, 18(3): 331-335.
Abstract:
ICT-2000TE system was used to investigate the stem sap flow of Acacia mangium, an important species for afforestation in south China, in 2003. The characteristics of stem sap flow change, the daily change of sap flow amount, the sap flow in different points and yearly fluctuation were analyzed. The results showed that the daily sap flow amount followed a monopeak curve. The amounts of daily total sap flow in rainy season and dry season were 10.988×103 g and 5.082×103 g respectively and the yearly total was 2 238.265×103 g. There esisted significant differences among different positions of A. mangium in water transferring capacity. The position with the fastest sap flow located at the xylem of 15 mm. The daily accumulated sap flow amounts of sapwood in rainy season and dry season were 85.203×103 g·m-2 and 57.463×103 g·m-2 respectively. The entire process of daily sap flow presented a S-shape curve.
Effects of Soil Salt Stress on the Activity of Protective Enzymes and Peroxidation in Three Warm-season Turfgrasses
ZHOU Xing-yuan, CAO Fu-liang
2005, 18(3): 336-341.
Abstract:
Activity of protective enzymes and peroxidation in three warm-season turfgrasses under soil salt stress were studied. The results showed:the activities of several protective enzymes(SOD, CAT, POD and APX) of Centipedegrass under 0.6%NFA480.8% salt stress declined gradually with the stress prolonged, while the activities of protective enzymes increased in 0.2%NFA480.4% salt stress of Centipedegrass and all the treatments of Zoysiagrass and Manilagrass. MDA and RPP increased actually with the stress enhanced and prolonged, The increasing speed in Centipedegrass was the highest and in Manilagrass was the lowest. Activity of PAL increased with salt concentration increased in three turfgrasses, but under 0.8% salt stress, it increased in early phase of stress and decreased with the stress prolonged in Centipedegrass. The activity of protective enzymes and contents of MDA and RPP were related to the salt tolerance.It was considered that salt tolerance of Manilagrass was the highest and that of Centipedegrass the lowest.
Study on the Difference of Chemical Properties among Five Acacia Woods Ⅱ. Study on pH Values and Buffering Capacities
HUANG Luo-hua, QIN Te-fu
2005, 18(3): 342-344.
Abstract:
The pH values and buffering capacities for the five kinds of acacia woods(Acacia auriculaeformis,A.crassicarpa,A.cincinnata,A.mangium and A.melanoxylon) were investigated. The results were as follows: (1) there was a slight difference among the pH values of the woods, while a significant difference among the buffering capacities, the values ranged from 4.68 to 5.90;there was a significant difference in pH values between the sapwood and the heartwood of A. crassicarpa; (2) the acid buffering capacities in the woods changed between 2.70 mL and 8.35 mL, however,the base buffering capacities of the woods changed between 21.05 mL and 42.00 mL; (3) the curves of the buffering capacities in the woods were slightly different.
Introduction and Conservation of Casuarina Trees in China
ZHONG Chong-lu, BAI Jia-yu, ZHANG Yong
2005, 18(3): 345-350.
Abstract:
There are four genera and 96 species belonging to Casuarinaceae. The four genera are Casuarina, Allocasuarina, Ceuthostoma and Gymnostoma. Casuarina plants are naturally distributed in Australia, Southeast Asia and archipelagos in Pacific Ocean, with altitude from sea level to over 3 000 meters. Although casuarina trees were introduced into China in 1897, it was in mid 1980s that casuarina trees began to be introduced into China systematically. Then casuarinas genetic resources were collected, reserved and selected in provenance/family/clone levels. And some relative research had been developed on clonal propagation, symbiotic microbe, and so on. Up to now, 2 genera, 23 tree species, more than 200 provenances, 260 families and a lot of clones were introduced into China. By a series of field experiments, some better species, provenances and families for different sites in southern China were screened out, of which genetic resources of Casuarina equisetifolia and C. junghuhniana was in whole naturally distributed regions of provenances and secondary provenances. Since 1990, introduction of Allocasuarina tree species have been paid more attentions to, more than 10 tree species have been introduced into China and have been tested in southern China and southwestern China, which enriched Chinese casuarinas genetic resources. Now the casuarinas tree species introduced are as follows: Allocasuarina campestris, A. dielsiana, A. distyla, A. fraseriana, A. huegeliana, A. humilis, A. littoralis, A. luehmannii, A. nana, A. paludosa, A. pinaster, A. striata, A. torulosa, A. verticillata, Casuarina collina, C. cristata, C. cunninghamiana, C. equisetifolia, C. glauca, C. grandis, C. junghuhniana, C. obesa and C. oligodon. In this paper, casuarinas introduction history, current research, conservation and utilization were reported.
Soil Ecological Management of Cultivated Bamboo Stand
CHEN Shuang-lin, XIAO Jiang-hua
2005, 18(3): 351-355.
Abstract:
Guided by the theory of sustainable development, and based on the concept of forest soil ecological management, the authors reviewed the history of bamboo stand soil management in China, analyzed the negative influence of existing main measures of bamboo stand management on soil ecological quality, put forward the study directions of bamboo stand soil ecological management in the future.
Research on Cultivation Pattern of Vegetation Recovery at Waste Heap Platform of Coal Mine
SUN Cui-ling, SU Tie-cheng, GUO Yu-wen
2005, 18(3): 356-361.
Abstract:
In order to study 5 cultivation models such as arbor-shrub, arbor-shrub-herb and their disposition for vegetation recovery, 10 arbor species, 7 shrub species and 5 herb species suitalde for locat sites were choiced at waste heap platform of Fushun Coal Mine. The experiment adopted the randomized block design, three replicates, with 0.067 hm2 at each thinned plot.
The Relationship between the Tail Morphology of Adult Female Bursaphelenchus xylophilus and Its Host
ZHAO Wen-xia, YANG Bao-jun
2005, 18(3): 362-363.
Abstract:
Terminal mucro of adult female was the distinct character between Bursaphelenchus xylophilus (Bx) and its allied species Bursaphelenchus mucronatus(Bb). In general, Bx had no mucro. But it could have mucro in some situations. In the test, Bx without mucro was inoculated on Chineses pine (Pinus tabulaeformis) and Japanese black pine (Pinus thunbergii). The results showed that 85% of adult females Bx isolated from Chinese pine had terminal mucro. On Japanese black pine only 4.2% of adult females Bx had terminal mucro. Bx with terminal mucro from Chinese pine was re-inoculated on the fungus, Pestalotia sp. and Japanese black pine. It was found that 100% adult females had no terminal mucro on the fungus and 3.4% with terminal mucro on Japanese black pine.
Transformation of Poplar (Populus alba × P. glandulosa cv.‘84K’) with Binary Insect Resistant Genes and Analysis of Insect Resistance
ZHANG Bing-yu, SU Xiao-hua, LI Yi-liang, ZHANG Yong-an, QU Liang-jian, WANG Yu-zhu, TIAN Ying-chuan
2005, 18(3): 364-368.
Abstract:
Transgenic poplar(Populus alba×P.glandulosa cv‘84k’) plants with the coleopterous insect resistant genes (BtCry3A and OC-I)were obtained. The transgenic nature of these plants was confirmed by PCR amplification and dot hybridization. The transgenic poplar’s toxicity towards the Anoplophora glabripennis larvae was assessed on two year-old selected plants in laboratory conditions. The results indicated that the transgenic lines BOGA-38 and BOGA-39 were deleterious for A. glabripennis larvae, and BOGA-5、BOGA-31、BOGA-38、BOGA-39 could inhabit the growth of A. glabripennis larvae. BOGA-39 was the most toxic one among the transgenic lines, with (41.18 %) corected mortality rate and 78.90 % growth inhabit rate for the larvae.
2005, 18(3): 369-369.
Abstract: