• 中国中文核心期刊
  • 中国科学引文数据库(CSCD)核心库来源期刊
  • 中国科技论文统计源期刊(CJCR)
  • 第二届国家期刊奖提名奖

2009 Vol. 22, No. 6

Display Method:
Study on the Medium Components Affection Pollen Germination and\nPollen Tube Growth of Corylus heterophylla×C.avellana
2009, 22(6): 753-757.
Abstract:
Solid medium was using to study the effect of sugar, boron, calcium on pollen germination and pollen tube growth of Corylus heterophylla Farnch.×C.avellana L.. The results showed that: different concentrations of sucrose and boric acid had significant impact on pollen germination, but different concentrations of calcium had no significant impact on pollen germination. It was most appropriate for tube elongation when sugar 150 g·L-1 and boric acid 100 mg·L-1. Lack of sucrose would lead to the accumulation of callose in top of pollen tube and hold back its growth. The distribution of callose was regular in pollen tube when the medium lacked boric. The appropriate medium for pollen germination of C.heterophylla×C.avellana was sugar 150 g·L-1 + boric acid 100 mg·L-1 + CaCl2100 mg·L-1 + agar 10 g ·L-1.
Genetic Variation in Growth, Wood Density and Bark Thickness of\n Eucalyptus dunnii
2009, 22(6): 758-764.
Abstract:
Two Eucalyptus dunnii provenance-family trials that included 53 families representing 6 provenances at Guilin and Liuzhou in Guangxi, were used to study the genetic variation and genotype by environment interaction for diameter at breast height (DBH) at 8.5-years, and DBH, height, stem volume, wood density and bark thickness at 10.5-years. The 10.5 year results showed: the mean volumes of individual trees at Guilin and Liuzhou were 0.218 and 0.189 m3, the average wood basic densities were 521.0 and 483.7 kg·m-3 respectively; and, the average bark thicknesses at breast height were 1.15 and 1.26 cm respectively. For the 10.5-years growth traits and wood density, there were no significant differences among provenances (P>0.10), but significant differences among families (within provenances) for 10.5-years wood density (PP>0.10) at any level for bark thickness at breast height, including no significant genotype by environment interactions for this trait. There were no significant interaction between sites and provenances (P>0.10) for all the traits except 8.5-years DBH,but significant interactions between sites and families (within provenance)(P<0.05)were found for all the traits studied except 10.5-years DBH. The heritabilities of DBH at 8.5-years and 10.5-years DBH and height were between 0.128 9-0.234 5, the heritability of wood basic density were 0.700 0-0.962 9, bark thickness were 0.105 3-0.335 7.
Selection of Models of Photosynthesis in Response to Irradiance and Definition\n of Attribute of Weak Light
2009, 22(6): 765-771.
Abstract:
Parabola, rectangular hyperbola, non-rectangular hyperbola, modified rectangular hyperbola and a subsection function were adopted to model the light-response curve for three different types of vegetations in the dry-hot river valley of Jinsha River, and a method was built to confirm the weak light region which related to apparent quantum yield of photosynthesis. Three main conclusions were gotten: (1)In five simulation methods, only subsection function and modified rectangular hyperbola had good simulation effect for three different types of light response curves; (2)The parameters Pmax,LCP, and Rd from rectangular hyperbola and non-rectangular hyperbola were near to the observations, but the estimates of LSP were always far lower than the observations, modified rectangular hyperbola was adapted to model the curves with phenomenon of light restrain and the estimation of photosynthetic parameters, but this method could not get the photosynthetic parameters for up-raising curve, the parameters estimated by subsection function for different types of curves were all near to the observations; (3) It was relatively theoretical to view the light range divided by the descending point of light-using efficiency on light response curve as the weak light range, the apparent quantum yield of photosynthesis was often lowly estimated when the weak light region could not be confirmed.
Molecular Characterization of Elite Genotypes within A First Generation \nBreeding Population of Pinus massoniana Using ISSR
2009, 22(6): 772-778.
Abstract:
By using 12 selected ISSR (inter-simple sequence repeat)markers, 78 first generation parents from different provenances of Pinus massoniana were assessed to detect their genetic differences. It was found that the percentage of population polymorphic loci, the Neis genetic diversity and the Shannons information diversity index were high, which indicated that the genetic variation of the first generation breeding population was at high level.The genetic distances ranged from 0.115 to 0.776 with an average genetic distance of 0.371. The genetic distance between parents with large latitudinal distance was greater than that with small latitudinal distance. The geographical latitudinal distance between parents of most superior cross combinations was large, and the genetic distance of most parents were greater than 0.430, indicating the parents with both large latitudinal distance and great genetic distance had the largest potential to exhibiting heterosis. It was proposed that elite trees with both large genetic distance and large geographical latitudinal distance should be preferentially considered in the selection of parents and construction of advanced generation breeding population, to maintain high level of genetic variability and improve the genetic gain of breeding.
Nutritional Composition and Its Genetic Variance of\nPhyllostachys iridescens Shoots
2009, 22(6): 779-783.
Abstract:
Nutritional composition and its genetic variance of Phyllostachys iridescens shoots were studied. The results showed that crude protein, soluble sugar and crude fat were 31.73%, 5.11%, 4.44%, respectively; total amino acids were 327.81 mg.g-1, among which 35.95% were essential amino acids; mineral elements were N 5 079.31 mg·kg-1 , P 6 037.40 mg·kg-1 , K 45 169.40 mg·kg-1 g, Fe 70.68 mg·kg-1 , Zn 78.60 mg·kg-1 , Ca 168.56 mg·kg-1 , Mg 1 443.76 mg·kg-1 , Cu 17.32 mg·kg-1 , Se 0.88 mg·kg-1 , respectively. Composition of crude protein, soluble sugar and crude fat were significantly different among geographical sites, all of their genetic variance were higher than environmental variance, crude protein had the lowest genetic variance coefficient, each with broad heritability of 88.69%,63.07%and 74.74%. As for the 17 amino acids detected in shoots, Glu was top with 37.92 mg·g-1, the second was Asp with 35.45 mg·g-1, and Met was the lowest with 3.29 mg·g-1. Only Se of 9 mineral elements had a high variance coefficient of 26.51%, others were no more than 13.11%. No evident correlation existed between shoots and local soil in the amount of mineral elements among different geographical sites. Some correlation existed among 3 organic nutrition and 9 mineral elements, for example, evident positive correlation existed between crude protein to K and Zn, soluble sugar to Fe, and K to Mg.
Two Species of Inonotus (Hymenochaetaceae) New to China
2009, 22(6): 784-787.
Abstract:
Two species of Inonotus: Inonotus rickii and Inonotus plorans were newly recorded from China. Inonotus rickii was found on Hevea brasiliensis from Hainan Province, southern China. Inonotus plorans was recently found on Populus sp. from western Uygur Xinjiang Autonomous Region of northwestern China. The illustrated descriptions of the two species were given based on the materials collected from China.
Study on Consociation Equation Set Model Method to Establish Duality\n Standing Tree Volume Model
2009, 22(6): 788-791.
Abstract:
Taking Larix gmelini as an example,the consociation equation set model method was used in creating duality standing tree volume model, the variance of constructing model and estimation precondition could be effectively solved to duality standing tree volume model. The usual nonlinear estimation and two steps estimations method were used to estimate the consociation equation set model method of duality standing tree volume model. As a result, the model could be estimated by two methods; two steps estimation method could reduce system error, and attain error limit than universal model, two steps estimation method was better than usual nonlinear estimation.
Thermal Effect of Rural Landscape Patterns
2009, 22(6): 792-800.
Abstract:
The thermal effect of landscape patterns from 50 villages in northern plain of Shaoxing County, Zhejiang Province was analyzed based on 2002 Quickbird and ETM images data. In the core area (build-up area) of villages, 45 landscape patterns indices (LPI) of 5 patches (land-use types including building, water, grass plot, woodland and green area mixed by grassplot, woodland) and whole landscape were calculated, and their correlation to environment brightness temperature (EBT) were discussed. The result showed that there was a sound linear relationship between the LPI and BET; the LPI studied here could be classified into 6 groups, i.e. density index, coverage index, shape index, core area size, indices of 100 m wide buffer ring and others, of which the indices type of density, coverage aggregation of dominant patch or of whole landscape have more significant correlation with BET; the indices about building patch and core area size (coverage, mean patch size and aggregation of building patches, area, perimeter and mean patch size of core area) and the shape indices of temperature-reducing patches had positive correlation with BET, others had negative correlation. So those villages, small size in built-up area, small size and even disperse in patches and narrow shape in buildings, were helpful for environment temperature reducing. 4 empirical models were established (R2>0.9) referring to 4 groups of LPI for predicting and adjusting the EBT being selected. However, the models fitted with single landscape quantity structure index had low performance. It means that the contribution of landscape patterns indices to environment temperature, up to about 25% of total, can not be ignored in village built-up area.
Assessment on Heavy Metal Pollution in Sediment of Xixi National \nWetland Park in Hangzhou City
2009, 22(6): 801-806.
Abstract:
Heavy metals in sediments of Xixi Wetland were researched. Effects of protection measures, sediment dredging and water types on heavy metal content were discussed. The results indicated that mean contents of Cu, Zn, Cr, Pb, Cd, Hg and As were lower than the second grade standard of Chinese environmental quality standards for soils (GB 15618-1995). Hg and As were lower than the background values of agriculture soils in Hangzhou City, while Zn, Cr, Pb and Cd were higher than that. The various correlations of heavy metals indicated a multi-source of sediment pollutants. Zn and Cd contents in sediments were higher than that in soils, which indicated different sources of heavy metals in sediment and soils. Spatially, the pollutant contents were lower in center wetland (first phase project) than that in outside. And the accumulation of heavy metals had been reduced in center wetland, while it was improved in outside areas which were being disturbed by human activities more frequently. Heavy metal contents were higher in sediment of closed pounds or big pools than that in general watercourses as water types had also effects on the heavy metal contents in sediment. The H kanson integrated potential ecological risk of Xixi was light as the mean integrated index was low (110.9). Cd and Hg were in medium levels, which had the heaviest contribution to integrated potential ecological risk, while other 5 metals had low levels of risk. And the results concluded from American National Oceanic and Atmospheric Administration of Sediment Quality Guidelines for the sediment risk assessment was different comparing with the H kanson's.
Predictability Time Scale and Fractal Characteristics of Temporal \nVariation of Eucommia ulmoides Plantation Transpiration
2009, 22(6): 807-812.
Abstract:
A case study with Eucommia ulmoides plantation in the mountainous region of North-China, predictability and fractal characteristics of temporal variation of trees transpiration were analyzed by using the method of reconstructing phase space when the lag time is 30 minutes. Transpiration measured with the technique of thermal dissipation probes in 2005 and 2006, The results showed that temporal variation of the plantation transpiration is a chaotic motion and characterized with fractal pattern and predictability during the main growth season. The fractal dimension (D), Renyi entropy (K2), embedding dimensions and the average predictability time scale of time series for the trees transpiration were 1.673, 0.002 49, 9 and 401.0 minutes in 2005,respectively, and 1.525, 0.002 53, 10 and 394.8 minutes in 2006. There existed significant difference in external appearance of temporal variation or in the amount of transpiration between the two years, but no difference about D and K2 at the same lag time. It is concluded that there were no differences in natural characteristics or internal patterns of time series in different years.
Research on Visual Simulation Technique of Chinese Fir Plantation Thinning
2009, 22(6): 813-818.
Abstract:
With the Chinese fir,Cunninghamia lanceolata,plantation in Huangfengqiao Forest Farm of Hunan Province, as the research object, and from the viewpoint of forest thinning, the principles and methods of forest management visualization were studied and a forest management-oriented virtual forest prototype was developed. The system used forest inventory data as data source and achieved the functions of virtual forest roaming, information query of subcompartment and forest stand thinning simulation, etc. so it was possible to offer a new platform for forest resource management and its visualization.
Study on the Biological Characteristics of Stain Fungi and Mould\n Fungi on Bamboo Wood
2009, 22(6): 819-823.
Abstract:
78 fungal isolates, identified as Trichoderma spp., Rhizomucor spp., Aspergillus spp., Fusarium spp., Alternaria spp., Nigrospora oryzae and Botryosphaeria rhodina, respectively, were isolated from stained bamboo samples from North, South and Centre of China. The represent isolates were selected to conduct the biological characteristics study. The nutrition N and C had close relation to the hyphae growth and pigment secretion. Most of the fungi grew well at 28 ℃, and grew very slowly but not died at 5 ℃, at 40 ℃ grew slowly and nearly not survive. Isolate Y-50 survived and grew at 50 ℃, which showed that it had ability of surviving in extreme environment. The majority of fungi could grow at the condition of pH value 4 - 11 and the proper pH value was 5 - 8. The light factor had no significant impact on the growth of the fungi.
Comparison of Annual Individual-Tree Growth Models Based\n on Variable Rate and Constant Rate Methods
2009, 22(6): 824-828.
Abstract:
The inventory data of the Chinese pine (Pinus tabulaeformis) in Beijing Mountains were used to establish and compare individual tree diameter growth and survival models with constant rate method and variable rate method. Results showed that in the individual-tree diameter growth model, MD was -0.005 9, MAD 0.779 1, RMSE 1.198 3 and R2 0.949 3 based on the variable rate method. But with the constant rate method, they were 0.097 5, 0.917 8, 1.198 3 and 0.917 8 respectively. In the survival model, MD was 8.59E-7, MAD 0.088 4, RMSE 0.211 7, and LogL-4 137.240 0 using the variable rate method. But with the constant rate method, they were 0.002 7, 0.091 1, 0.212 1, and -4 230.320 0 respectively. The variable rate method out-performed the constant rate method in predicting future individual tree survival and diameter growth because the former accounted for the variable rate of annual diameter growth and tree survival probability, which were caused by changes of stand (basal area, dominant height) and tree attributes.
Influences of Drought Stress on the Physical Characteristics of Sedums Species
2009, 22(6): 829-834.
Abstract:
Through pot-cultivating seven Sedums species under drought stress, their drought tolerance to learned indexes, including the relative water content of leaves, the penetration of membrane and so on, were comprehensively evaluated and compared. The results showed, under the stress of continuous drought, the penetration of membranes and MDA had a increasing trend, superoxide dismutase activities increased with light drought stress and declined with severe drought stress. According to multiple analysis, the drought resistance of seven species were in the order of Sedum spurium 'Coccineum'> S.kamtschaticum Fisch > S. linear Thunb> S. reflexum Tripmadam > S. sarmentosum Bunge > S.spectabile Boreau> S. sexangulare L.
An Analysis of Relationship between Growth and Site Condition \nof Mytilaria laosensis Plantation
2009, 22(6): 835-839.
Abstract:
With the data of 79 sample plots collected from the plantation aged from 20—25 years in Daqingshan, south-west Guangxi province, China, an analysis on the relationship between growth and site conditions, including altitude, soil type, slope position, form and direction of slope, and site type, was conducted by analysis of variance (ANOVA). The result showed that the perfect site conditions for afforestation of Mytilaria laosensis would be the site: (1)with the altitude between 300—500 m in high hills or low mountains;(2)with the kraznozem which evolved from such magmatic rocks as granite and rhyolite;(3)at middle or lower part in a concave slope. If these could be met for site selection, the annual increment of height for dominant trees in stand would be 1.1—1.3 m and DBH would be 1.3—1.5 cm, and after 20 years of planting it would grow into a big-diameter timber forest.
Response of Fine Root Characteristics of Pinus massoniana to the Doses of\n Limestone Powder Mixed in Soil in the Acid Rain Area of Chongqing, China
2009, 22(6): 840-845.
Abstract:
The dose-effect of limestone powder on fine roots of Masson pine(Pinus massoniana) forest growing on acidified soil was studied at Tieshanping, Chongqing in Southwest China. Ten dominant trees of P. massoniana were selected in a 28-years-old stand for this study, and five treatments or application doses (134,261,388,515 and 642 g·m-3) of limestone powder were designed to be mixed in soil columns around the sampled trees. The invertigations were conducted before and 2 years after treatment. The results showed that the fine root characters of P.msaaoniana were significantly different after applying different doses of limestone power. With the increase of does, all the five character values showed a trend of decrease after increase. The character value after applying 388 g·m-3 of limestone powder was the maximum,indicating its best effect to promote the growth of root system,i.e. the length,surface area, root tip amount,volume and dry mass of fine roots were 600 m·m-3,130 dm2·m-3,150 000 tips. m-3,220 cm3·m-3, and 39 g·m-3,respectively.
Study on the Invasion of Eupatorium adenophorum in Pinus yunnanensis Community Damaged by Tomicus spp.
2009, 22(6): 846-850.
Abstract:
The invasion of Eupatorium adenophorum in Pinus yunnanensis stand damaged by Tomicus spp. was examined. The community structure, biodiversity index and invasion extent of three P. yunnanensis stands were determined and studied, and the correlation between Tomicus spp. damage and E. adenophorum invasion was analyzed. The results indicated that the Tomicus spp. damage could cause the change of habitat through affecting the structure of P. yunnanensis community, the existence of Tomicus spp. made the plant community more susceptive to the invasion of E. adenophorum. The probability of E. adenophorum invasion enhanced under simpler community structure, lower biodiversity and less sufficient use on habitat resources. The damage of Tomicus spp. caused the stand of P. yunnanensis degraded; the invasion of E. adenophorum further speeded up the degradation of ecological system. E. adenophorum invasion has become a critical ecological process which closely related to the healthy of forest ecological system.
Effects of Different Treatments on Soil Microenvironment and Germination\nRate of Gleditsia sinensis Afforested by Direct Seeding
2009, 22(6): 851-854.
Abstract:
Effects of different treatments,including mulching film, grass cover, block cover, water retaining agent, rooting powder and open field (control), on direct seeding of Gleditsia sinensis Lam. were studied. The results showed that mulching film increased daily average temperature and the effect order was sunlight > after-rain > cloud. The soil temperature of grass cover and block cover were lower than that of open field. After 6-day sunlight, soil moisture content of mulching film, grass cover and water retaining agent were higher than that of open field, with increased by 24.4,21.4,2.5 and 1.0 g·kg-1, respectively. 30 days after seeding, the germination rates with the methods of mulching film, rooting powder block cover, water retaining agent were higher than that of the control, with increases of 12.11%, 4.20%, 3.10%, and 2.10%, respectivey. While the germination rate of grass covering was lower than that of the control.
Evaluation of Economic Benefit of the Acacia mangium Plantation\n——A Case Study in Nanning, Guangxi
2009, 22(6): 855-859.
Abstract:
The combination of static and dynamic analysis methods were used to estimate the economic benefit of Acacia mangium plantation. The results showed that: at the cutting age of 6 years, the average annual investment profit margin was 11.5%, the financial internal rate of return was 22.4%, the net present value of the plantation was 2 375.3 yuan·hm-2 ,dynamic investment recovery period was 5.7 years, the price factor was the most sensitive factor affecting the income.
Research on Growth Process and Growth Models of Quercus veriabilis\nin Beijing Region
2009, 22(6): 860-864.
Abstract:
Based on the data of 8 analytical trees originated from 3 types tree source collected from Xishan and Dayanshang area of Beijing, the growth process of DBH, height and volume and the relationship between DBH and height were analyzed respectively; 4 theoretical equations were applied to fit the growth process. The results showed that the 3 types of sample trees were all in the fast growing stage which was far from quantity mature stage; the comparison of growth process among the 3 types of sample trees showed that the growth of trees originated from seedling sprouts were the fastest, followed by trees originated from natural regeneration and trees originated from planted seedlings, however, the trees originated from seedlings showed larger growth potential than those originated from seedlings sprouts; the results of DBH-height relationship showed a positive correlation trend; but the height growth of trees originated from seedling sprouts became slower when the DBH larger than 7 cm; the results of 4 fitted theoretical equations showed that Richards,Mitscherlich and Gompertz equations well fitted the growth processes of DBH, height and volume of the sample trees, while the fitting precision of Logistic equation was relatively lower. This study can provide reference for the management of Quercus variabilis forest in Beijing.
Correlation between Health Conditions of Pinus yunnanensis Secondary\n Forest and Soil Properties in Golden Temple Forest Region of Kunming
2009, 22(6): 865-871.
Abstract:
Soil properties of healthy, sub-healthy and unhealthy Pinus yunnanensis secondary forest were measured and their correlation with the health conditions of Pinus yunnanensis forest were analyzed. The results revealed that, the soil water content,bulk density, porosity, sand content, cosmid content, CEC and radily available K content changed regularly with the health condition changes of Pinus yunnanensis forest. Correlation analysis revealed that, there were positive correlation between soil water content, porosity, cosmid content, CEC, radily available K content and health conditions of Pinus yunnanensis forest; and negative correlation between bulk density, sand content, meal content and health conditions of Pinus yunnanensis forest. By stepwise regression analysis, the regression models about health conditions of Pinus yunnanensis forest and soil properties were established.
Seasonal Photosynthetic Variance of Phyllostachys edulis\n 'Pachyloen' Response to Environmental Factors
2009, 22(6): 872-877.
Abstract:
The seasonal photosynthetic response of Phyllostachys edulis 'Pachyloen' to light intensity, CO2 concentration, temperature and humidity were measured with Li-6400P. The main study results were as follows:The seasonal photosynthetic variation tendency of Ph. edulis 'Pachyloen' to light intensity was summer>autumn>winter>spring for Pmax, spring>autumn>winter>summer for LCP, autumn>summer>winter>spring for LSP, and summer> autumn>winter>spring for AQY. The seasonal photosynthetic variation tendency of Ph. edulis 'Pachyloen' to CO2 concentration was spring>autumn>summer> winter for LCP and CCP, and summer>autumn> winter>spring for CE. The optimal temperature for photosynthesis of Ph. edulis 'Pachyloen' ranged from 20 to 30 ℃, equivalent in spring and autumn, higher in summer and lowest in winter. The optimal humidity for photosynthesis of Ph. edulis 'Pachyloen' ranged from 40% to 65%, its seasonal variation tendency was autumn>summer>winter>spring. As a cultivar of Ph. edulis, the characteristics of photosynthetic responses of Ph. edulis 'Pachyloen' to environmental factors were similar with Ph. edulis. It indicated their close phylogenetic relationships.
Study on Additives of VBL,Chlorbenzuron,Trichlorphon on\n Parocneria orienta NPV
2009, 22(6): 878-882.
Abstract:
Bioassay of PoNPV+1%VBL, PoNPV+12.5 μL·L-1 Chlorbenzuron, PoNPV+15 μL·L-1 Trichlorphon was conducted on 2-instar larvae of Parocneria orientia in lab. The results showed that 1%VBL, 12.5 μL·L-1 Chlorbenzuron, 15 μL·L-1 Trichlorphon had better synergistic effect. Spraying of PoNPV+1%VBL, PoNPV+12.5 μL·L-1 Chlorbenzuron, PoNPV+15 μL·L-1 Trichlorphon and PoNPV to control 2 - 3 instar larvae of second generation in field, the concentrations of PoNPV were 8×106 PIB·mL-1, 4×106 PIB·mL-1, 2×106 PIB·mL -1, 1×106 PIB·mL -1, 5×105 PIB·mL -1, there were 20 treatments. It was indicated that when the concentration was the same, the effect of PoNPV added synergists was better than that without synergists.
The Ecological Distribution and Resources of Macrofungi \nin Daliangzihe National Forest Park
2009, 22(6): 883-887.
Abstract:
Macrofungi in the Daliangzihe National Forest Park of Heilongjiang Province were investigated, and 170 species of macrofungi were identified. These fungi belong to 9 orders, 31 families and 90 genera. Among them, 50 species were edible mushrooms, 31 species were medicinal fungi, 13 species were poisonous mushrooms, 42 species were mycorrhizal fungi and 56 species were wood decaying fungi. The distribution of these fungi in the korean pine and broad-leaved mixed forest, korean pine and spruce mixed forest followed certain patterns, the population structures and abundance of these fungi were closely related to some forest factors including the structure of stands, soil, altitude and slope etc.
Preliminary Study on Shoots Regeneration from Tender Stems of\n Malus komarovii (Sarg.) Rehd. and Germplasm Reservation in vitro
2009, 22(6): 888-892.
Abstract:
The tender stems of Malus komarovii were used as explant and the suitable medium compositions were screened through uniform design experiments. The results showed that tissue culture of M. komarovii required different kinds of culture medium in different phases. SH+TDZ 2.45 mg·L-1+NAA 0.06 mg·L-1 for shoots regeneration immediately at base of tender stem, the rate of induction was 98.8%; 1/4 SH+KT 0.35 mg·L-1+NAA 0.05 mg·L-1 for rooting, the rate of rooting was more than 97%; 1/8 SH+ABA 2.05 mg·L-1+KT 0.50 mg·L-1 for germplasm preservation in vitro, the rate of dormancy was 95.5%. These plant materials could be maintained for 39 months by the methods of promoting dormancy and low nutrients at normal temperature.