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Citation:

Establishment of Cell Suspension Culture and Isolation of Protoplast of Pinus serotina

  • Received Date: 2001-09-18
  • Callus was induced by embryos from mature seeds of Pinus serotina with MS medium contained 0.2 mg·L-1 of KT and 2.5 mg·L-1 of 2,4 D.After successive subculture and selection,six month old callus were transferred to a liquid medium with 0.2 mg·L-1 of KT and 0 5.0 mg·L-1 of 2,4 D and 200 mg·L-1 of LH for vibration culture,the suspension cultured cell lines were established with favorable dispersivity.A great amount of protoplasts were released from three month old cell suspension cultures,and protoplast yield and protoplast viability of suspension cells on linear phase are the highest under the enzyme combination cellulase R 10.2%+pectinase 0.25%?
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Establishment of Cell Suspension Culture and Isolation of Protoplast of Pinus serotina

  • 1. The Research Institute of Subtropical Forestry, CAF, Fuyang 311400, Zhejiang, China

Abstract: Callus was induced by embryos from mature seeds of Pinus serotina with MS medium contained 0.2 mg·L-1 of KT and 2.5 mg·L-1 of 2,4 D.After successive subculture and selection,six month old callus were transferred to a liquid medium with 0.2 mg·L-1 of KT and 0 5.0 mg·L-1 of 2,4 D and 200 mg·L-1 of LH for vibration culture,the suspension cultured cell lines were established with favorable dispersivity.A great amount of protoplasts were released from three month old cell suspension cultures,and protoplast yield and protoplast viability of suspension cells on linear phase are the highest under the enzyme combination cellulase R 10.2%+pectinase 0.25%?

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