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Citation:

Factors Effecting on Tissue Culture of Perennial Ryegrass ( Lolium perenne L.)

  • Received Date: 2003-05-06
  • Plantlets were regenerated from seed-derived callus of perennial ryegrass (Lolium perenne). The replacements of 2,4-D by dicamba, maltose by sucrose in the callus induction medium significantly improved callus induction frequency and overall plant regeneration ability. From 3 mg·L-1 to 9 mg·L-1, the stepwise increase of the 2,4-D concentration in maltose containing callus induction medium obviously increased callus induction frequency but decreased callus differentiation frequency. The callus induction medium supplemented with two auxins (2,4-D and NAA) was much better than that with 2,4-D individual. Elevating concentrations of casein hydrolysate, proline and glutamine did not improve overall plant regeneration frequency.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Factors Effecting on Tissue Culture of Perennial Ryegrass ( Lolium perenne L.)

  • 1. College of Life Science, Zhejiang University, Hangzhou310012, Zhejiang, China
  • 2.  China National Rice Research Institute, Hangzhou310006, Zhejiang, China

Abstract: Plantlets were regenerated from seed-derived callus of perennial ryegrass (Lolium perenne). The replacements of 2,4-D by dicamba, maltose by sucrose in the callus induction medium significantly improved callus induction frequency and overall plant regeneration ability. From 3 mg·L-1 to 9 mg·L-1, the stepwise increase of the 2,4-D concentration in maltose containing callus induction medium obviously increased callus induction frequency but decreased callus differentiation frequency. The callus induction medium supplemented with two auxins (2,4-D and NAA) was much better than that with 2,4-D individual. Elevating concentrations of casein hydrolysate, proline and glutamine did not improve overall plant regeneration frequency.

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