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DNA Molecular Evidences of the Inter-specific Hybrids between Paeonia ostii and P.suffruticosa Based on ISSR Markers

  • Received Date: 2004-03-10
  • Paeonia ostii was believed to be one of the main progenitors of the Chinese tree peony cultivars,and hybridization was the most important pathway for incorporation of genetic composition of P.ostii.However,no experimental evidence had been reported till now.In this study,P.ostii was used as a maternal parent crossing with other two Chinese tree peony cultivars which were used as paternal parents.The relationships between the F1 hybrids and their parents were analyzed by means of DNA fingerprinting based on ISSR (Inter-simple sequence repeats) markers,and ISSR fragments typically inherited from both parents were detected in the genomes of the F1 generation.This study also indicated that ISSR marker was suitable for identification of F1 hybrids in P.suffruticosa at seedling stage.
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通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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DNA Molecular Evidences of the Inter-specific Hybrids between Paeonia ostii and P.suffruticosa Based on ISSR Markers

  • 1. Institute of Botany, Chinese Academy of Sciences, Beijing100093, China
  • 2. Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing100093, China

Abstract: Paeonia ostii was believed to be one of the main progenitors of the Chinese tree peony cultivars,and hybridization was the most important pathway for incorporation of genetic composition of P.ostii.However,no experimental evidence had been reported till now.In this study,P.ostii was used as a maternal parent crossing with other two Chinese tree peony cultivars which were used as paternal parents.The relationships between the F1 hybrids and their parents were analyzed by means of DNA fingerprinting based on ISSR (Inter-simple sequence repeats) markers,and ISSR fragments typically inherited from both parents were detected in the genomes of the F1 generation.This study also indicated that ISSR marker was suitable for identification of F1 hybrids in P.suffruticosa at seedling stage.

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