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Citation:

Genomic DNA Extraction and RAPD Protocols for Rattan

  • Received Date: 2003-12-08
  • Genomic DNA was successfully extracted from leaf tissues of two rattan species,Calamus simplificifolius and Daemonorops margaritae,using a modified CTAB method.This method could remove phenolic compounds,polysaccharides and proteins,and the quality and quantity of DNA extracted were reliably characterized.In addition,the reaction composition and amplification program for RAPD analysis were developed with GeneAmp PCR System 2700 (Applied Biosystem Co.).This study provided a technological basis for future molecular studies in rattan and the related species.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Genomic DNA Extraction and RAPD Protocols for Rattan

  • 1. Research Institute of Tropical Forestry, CAF, Guangzhou510520, Guangdong, China

Abstract: Genomic DNA was successfully extracted from leaf tissues of two rattan species,Calamus simplificifolius and Daemonorops margaritae,using a modified CTAB method.This method could remove phenolic compounds,polysaccharides and proteins,and the quality and quantity of DNA extracted were reliably characterized.In addition,the reaction composition and amplification program for RAPD analysis were developed with GeneAmp PCR System 2700 (Applied Biosystem Co.).This study provided a technological basis for future molecular studies in rattan and the related species.

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