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Citation:

Molecular Cloning of Phenylalanine Ammonia-lyase in Ginkgo biloba

  • Received Date: 2004-12-02
  • According to the conservative domain of some other plants DNA sequences of PAL gene,a pair of degenerate primer was designed.A special DNA fragment was obtained by PCR amplifying,which length is 862 bp,and it is named Gbpal1.We analysed the nucleotide and amino acid sequence of smilarity to the corresponding gene of other organism,the results showed that Gbpal1 is a partial PAL gene of Ginkgo biloba.The sequence had been embodied in Genebank,and Accession NO.is AY578145.The southern blot analysis indicated that Ginkgo biloba PAL belonged to a multigene family.
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通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Molecular Cloning of Phenylalanine Ammonia-lyase in Ginkgo biloba

  • 1. Fruit Science Institute of Zhejiang University,Hangzhou 310000,Zhejiang,China
  • 2. College of Life Science,Yangtze University,Jingzhou 434025,Hubei,China
  • 3. College of Gardening and Horticulture,Yangtze University,Jingzhou 434025,Hubei,China

Abstract: According to the conservative domain of some other plants DNA sequences of PAL gene,a pair of degenerate primer was designed.A special DNA fragment was obtained by PCR amplifying,which length is 862 bp,and it is named Gbpal1.We analysed the nucleotide and amino acid sequence of smilarity to the corresponding gene of other organism,the results showed that Gbpal1 is a partial PAL gene of Ginkgo biloba.The sequence had been embodied in Genebank,and Accession NO.is AY578145.The southern blot analysis indicated that Ginkgo biloba PAL belonged to a multigene family.

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