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Citation:

FISH-AFLP Analysis of Genetic Diversity on Walnut Cultivars in Yunnan Province

  • Received Date: 2010-07-05
  • The genetic diversities of 17 walnut cultivars(Juglans sigillata Dode) authorized or approved by Yunnan Province Forest Tree Cultivar Registration Committee were investigated with FISH-AFLP technical system. Eight pairs of E+3/M+3 primers were used to amplify the genomic DNA. The results showed that 987 AFLP bands were obtained, 942 of them were polymorphic markers and the average percent of polymorphic bands was 95.44%. The genetic similarity indexes among all the 17 cultivars are ranged from 0.606 9 to 0.796 4 with an average of 0.734 9. These investigations would be significant in utilizing walnut germplasm resources and cultivating new varieties.
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FISH-AFLP Analysis of Genetic Diversity on Walnut Cultivars in Yunnan Province

  • 1. Research Institute of Economic Forestry, Yunnan Academy of Forestry, Kunming 650204, Yunnan, China
  • 2. Research Institute of Forestry, Chinese Academy of Forestry
  • 3.  Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China

Abstract: The genetic diversities of 17 walnut cultivars(Juglans sigillata Dode) authorized or approved by Yunnan Province Forest Tree Cultivar Registration Committee were investigated with FISH-AFLP technical system. Eight pairs of E+3/M+3 primers were used to amplify the genomic DNA. The results showed that 987 AFLP bands were obtained, 942 of them were polymorphic markers and the average percent of polymorphic bands was 95.44%. The genetic similarity indexes among all the 17 cultivars are ranged from 0.606 9 to 0.796 4 with an average of 0.734 9. These investigations would be significant in utilizing walnut germplasm resources and cultivating new varieties.

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