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Cloning and Expression Analysis of A Glycine-rich RNA-binding Protein Gene from Tamarix hispida

  • Received Date: 2010-07-29
  • The full-length cDNA of a presumed glycine-rich ribonucleic acid (RNA) binding protein (ThGRP1) gene was isolated from a cDNA library of Tamarix hispida leaves. The ThGRP1 gene with 800 bp in length contains 432-base pair (bp) open reading frame (ORF) and encodes a 143-amino-acid polypeptide with a predicted molecular weight of 14.95 kDa and pI of 5.36. Expression of ThGRP1 in roots, stems and leaves of T. hispida with PEG, NaCl, NaHCO3 or CdCl2 treatments was studied using real-time PCR. The results showed that ThGRP1 can be induced by these stresses. Especially, the expression of ThGRP1 in roots of T. hispida was induced under all stresses at 6, 12, 24, 48 and 72 h.
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Cloning and Expression Analysis of A Glycine-rich RNA-binding Protein Gene from Tamarix hispida

  • 1. Key Laboratory of Forest Tree Genetic improvement and Biotechnology of Ministry of Education, Northeast Forestry University, Harbin 150040, Heilongjiang, China

Abstract: The full-length cDNA of a presumed glycine-rich ribonucleic acid (RNA) binding protein (ThGRP1) gene was isolated from a cDNA library of Tamarix hispida leaves. The ThGRP1 gene with 800 bp in length contains 432-base pair (bp) open reading frame (ORF) and encodes a 143-amino-acid polypeptide with a predicted molecular weight of 14.95 kDa and pI of 5.36. Expression of ThGRP1 in roots, stems and leaves of T. hispida with PEG, NaCl, NaHCO3 or CdCl2 treatments was studied using real-time PCR. The results showed that ThGRP1 can be induced by these stresses. Especially, the expression of ThGRP1 in roots of T. hispida was induced under all stresses at 6, 12, 24, 48 and 72 h.

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