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cDNA Cloning and Sequence Analysis of β1-Tubulin from Chinese White Wax Scale Ericerus pela Chavannes (Homopetera: Coccidae)

  • Received Date: 2011-01-29
  • A full-length cDNA of β1-tubulin gene from Ericerus pela was obtained by using RACE and RT-PCR methods. The size of β1-tubulin gene is 1492 bp, which contains an open reading frame of 1 344 bp that encoding 447 amino acid residues with 50.20 kda molecular weight and 4.75 isoelectric point. The putative amino acid sequences contain conserved sequences of β1-tubulin and the GTP-binding site. The phylogenetic analysis showed that β1-tubulin gene of E. pela shares high identity with β-tubulin homologues from other insects. The sequences of β1-tubulin gene of E. pela were submitted to GenBank and the accession number was assigned JF731244.
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cDNA Cloning and Sequence Analysis of β1-Tubulin from Chinese White Wax Scale Ericerus pela Chavannes (Homopetera: Coccidae)

  • 1. Research Institute of Resource Insects, Chinese Academy of Forestry, The Key laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650224, Yunnan, China
  • 2. Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Faculty of Conservation Biology, Southwest Forestry University, Kunming 650224, Yunnan, China

Abstract: A full-length cDNA of β1-tubulin gene from Ericerus pela was obtained by using RACE and RT-PCR methods. The size of β1-tubulin gene is 1492 bp, which contains an open reading frame of 1 344 bp that encoding 447 amino acid residues with 50.20 kda molecular weight and 4.75 isoelectric point. The putative amino acid sequences contain conserved sequences of β1-tubulin and the GTP-binding site. The phylogenetic analysis showed that β1-tubulin gene of E. pela shares high identity with β-tubulin homologues from other insects. The sequences of β1-tubulin gene of E. pela were submitted to GenBank and the accession number was assigned JF731244.

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