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Citation:

Cloning and Function Analysis of Tamarix hispida Lipid Transfer Protein under Stress

  • Received Date: 2011-11-16
  • In this study, a novel LTP gene which was named ThLTP was cloned from the cDNA library of Tamarix hispida root treated by 0.4 mol·L-1 NaCl. The sequence of ThLTP was 635 bp in full length, a protein with 116 amino acids residues. The authors examined the expression pattern of the ThLTP gene in leaf and root of T. hispida treated with 0.2 mol·L-1 NaCl, 150 μmol·L-1 CdCl2, 20% (W/V) PEG6000, 100 μmol·L-1 ABA and 4 ℃ stresses for different time using real time RT-PCR. The results showed that the ThLTP gene was induced by all the treatments in leaf and root except the treatment of 4 ℃ treated in root. ThLTP was inserted into a prokaryotic expression vector (pET32a) to produce the recombinant expression vector pET32a-LTP. The Escherichia coli BL21 (pET32a) could not live but the E. coli BL21 (pET32a-LTP) came to the maximum after 3 h delayed growth under the stress of 0.8% (W/V) NaCl and 20% (W/V) PEG6000. The results showed that ThLTP may be a salt- and drought-tolerant gene and enhance the stress tolerance of the recombinant.
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Cloning and Function Analysis of Tamarix hispida Lipid Transfer Protein under Stress

  • 1. Northeast Forestry University,State Key Laboratory of Forest Genetics and Tree Breeding, Harbin 150040, Heilongjiang,China

Abstract: In this study, a novel LTP gene which was named ThLTP was cloned from the cDNA library of Tamarix hispida root treated by 0.4 mol·L-1 NaCl. The sequence of ThLTP was 635 bp in full length, a protein with 116 amino acids residues. The authors examined the expression pattern of the ThLTP gene in leaf and root of T. hispida treated with 0.2 mol·L-1 NaCl, 150 μmol·L-1 CdCl2, 20% (W/V) PEG6000, 100 μmol·L-1 ABA and 4 ℃ stresses for different time using real time RT-PCR. The results showed that the ThLTP gene was induced by all the treatments in leaf and root except the treatment of 4 ℃ treated in root. ThLTP was inserted into a prokaryotic expression vector (pET32a) to produce the recombinant expression vector pET32a-LTP. The Escherichia coli BL21 (pET32a) could not live but the E. coli BL21 (pET32a-LTP) came to the maximum after 3 h delayed growth under the stress of 0.8% (W/V) NaCl and 20% (W/V) PEG6000. The results showed that ThLTP may be a salt- and drought-tolerant gene and enhance the stress tolerance of the recombinant.

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