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Study on the Difference in Expression Profiles of microRNA Among Leaf Organs of Triploid and Diploid Populus×euramericana cl.‘Zhonglin46’

  • Received Date: 2013-08-20
  • Using total RNA extracted by CTAB method as templates, the expression profiles of 5 miRNAs in young and mature leaf organs of Populus×euramericana cl.‘Zhonglin46’were studied by real-time stem-loop RT-PCR. The results showed that the expression of miR159, miR167, miR171, and miR319 in triploid young leaves were higher than those in diploid, however, the expression of miR164 in mature leaves of triploid was higher than that in diploid. In addition, the expression analysis of the corresponding miRNA target genes showed that the expression of the miRNAs and their corresponding targets were negative correlation in diploid and triploid P.×euramericana cl.‘Zhonglin46’. these findings suggested that these miRNA target genes may be related to the formation of fast-growing traits showed by triploid Populus.
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Study on the Difference in Expression Profiles of microRNA Among Leaf Organs of Triploid and Diploid Populus×euramericana cl.‘Zhonglin46’

  • 1. College of Life Sciences, Nankai University, Tianjin 300071, China

Abstract: Using total RNA extracted by CTAB method as templates, the expression profiles of 5 miRNAs in young and mature leaf organs of Populus×euramericana cl.‘Zhonglin46’were studied by real-time stem-loop RT-PCR. The results showed that the expression of miR159, miR167, miR171, and miR319 in triploid young leaves were higher than those in diploid, however, the expression of miR164 in mature leaves of triploid was higher than that in diploid. In addition, the expression analysis of the corresponding miRNA target genes showed that the expression of the miRNAs and their corresponding targets were negative correlation in diploid and triploid P.×euramericana cl.‘Zhonglin46’. these findings suggested that these miRNA target genes may be related to the formation of fast-growing traits showed by triploid Populus.

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