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Molecular Cloning and Function Analysis of PtSIP3 Gene from Populus×euramericana cl.‘Zhonglin 46’Triploid

  • Received Date: 2013-08-22
  • According to the preliminary results of microarray analysis, a high expression gene, PtSIP3, was cloned from Populus euramericana triploid, which generated from crosses between the maternal parent P. deltoides and the pollen parent P. nigra. The binary expression vector, pRI101- PtSIP3, was constructed and transformed into Arabidopsis. So far, the PtSIP3 transgenic plants were observed the remarkable phenotypic changes with inhibition of embryo and capsule development, improvement root development. Therefore, It is speculated that the rises of PtSIP3 expression may participate in the regulation of vegetative growth and promote root development in P. euramericana triploid.
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Molecular Cloning and Function Analysis of PtSIP3 Gene from Populus×euramericana cl.‘Zhonglin 46’Triploid

  • 1. College of Life Sciences, Nankai University, Tianjin 300071, China

Abstract: According to the preliminary results of microarray analysis, a high expression gene, PtSIP3, was cloned from Populus euramericana triploid, which generated from crosses between the maternal parent P. deltoides and the pollen parent P. nigra. The binary expression vector, pRI101- PtSIP3, was constructed and transformed into Arabidopsis. So far, the PtSIP3 transgenic plants were observed the remarkable phenotypic changes with inhibition of embryo and capsule development, improvement root development. Therefore, It is speculated that the rises of PtSIP3 expression may participate in the regulation of vegetative growth and promote root development in P. euramericana triploid.

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