Cloning and Expression of UDP-Glucose Dehydrogenase Cdna in Larix kaempferi

WANG Jing; LI Ai; WANG Chun-guo; SONG Wen-qin; CHEN Cheng-bin

Based on the data of Japanese larch Solexa transcriptome sequencing obtained by splicing uridine diphosphate glucose dehydrogenase (UDPGDH) of the original sequence, the sequence ends with primers designed in parental and offspring young larch needles leaves, the cDNA sequence of UDPGDH gene was successfully cloned. Sequence analysis showed that the gene contained an open reading frame (GenBank No.KF499528) of 1 443 bp encoding a putative protein o f 480 amino acids. The expression of parental and offspring UDPGDH gene was analyzed by qRT-PCR. Offspring has a much higher expression compare with their parents' and orthogonal offspring UDPGDH gene express higher than the reciprocal cross progeny. UDP-glucose dehydrogenase is an important enzyme in the formation of hemicellulose and pectin, the components of newly formed cell walls. It might support that UDPGDH gene's high expression is beneficial to the heterosis of offspring.

1. College of Life Sciences, Nankai University, Tianjin 300071, China

Received Date: 2013-08-21

Abstract: Based on the data of Japanese larch Solexa transcriptome sequencing obtained by splicing uridine diphosphate glucose dehydrogenase (UDPGDH) of the original sequence, the sequence ends with primers designed in parental and offspring young larch needles leaves, the cDNA sequence of UDPGDH gene was successfully cloned. Sequence analysis showed that the gene contained an open reading frame (GenBank No.KF499528) of 1 443 bp encoding a putative protein o f 480 amino acids. The expression of parental and offspring UDPGDH gene was analyzed by qRT-PCR. Offspring has a much higher expression compare with their parents' and orthogonal offspring UDPGDH gene express higher than the reciprocal cross progeny. UDP-glucose dehydrogenase is an important enzyme in the formation of hemicellulose and pectin, the components of newly formed cell walls. It might support that UDPGDH gene's high expression is beneficial to the heterosis of offspring.

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Reference (11)

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Cloning and Expression of UDP-Glucose Dehydrogenase Cdna in Larix kaempferi

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通讯作者: 陈斌, bchen63@163.com

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