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Citation:

Analysis on Differences of Protein in Stylar Canal During Early Pollination Associated with Self-incompatible Prunus domestica × armeniaca ‘Fengweimeigui’

  • Received Date: 2014-01-06
  • This study focused on the differences of protein in stylar canal between that from self-incompatible Prunus domestica × armeniaca ‘Fengweimeigui’ and that from cross-pollination (ACP) (‘Fengweimeigui’ × ‘Konglongdan’). Two dimension difference gel electrophoresis (2D-DIGE) and mass spectrum (MS) detection techniques were used in this study. The results showed that there were 79 different protein spots in stylar canal between that from self-pollination and that from cross-pollination, and there was a huge difference, more than double, in terms of protein expression among 43 protein spots, including 6 up-expressed and 37 down-expressed in self-pollination stylar canal compared with that in cross-pollination stylar canal. Only 34 protein spots were identified unambiguously through mass spectrometry analysis, including 5 kinds which were catalytic protein, defence/stress protein, and structural protein, respectively.
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  • [1] 李芳东,杜红岩,杨绍彬,等. ‘风味玫瑰'—极早熟杏李杂交新品种[J].中国果树,2007,(3):91-96.

    [2] ?27] 杨绍彬,李芳东,杜红岩,等.杏李种间杂交品种适宜授粉组合筛选试验[J].中国果树,2008(5):28-30.

    [3] 李芳东,杜红岩,杨绍彬,等.杏李种间杂交极早熟新品种风味玫瑰[J].中国果树,2007,(3):5-7.

    [4] 王熙龙,曹振强.风味玫瑰杏李早期丰产栽培技术[J]. 北方果树,2005(4):29.

    [5] 李小远,马玉磊,刘 阳,等.小报春不同授粉组合亲和性研究[J].西北植物学报,2012,32(12):2426-2431.

    [6] 雷家军,吴 超.卷丹与亚洲百合种间杂交花粉管生长的荧光观察[J].东北农业大学学报,2012,43(1):117-120.

    [7] 罗春香,缑红星,盛瑞芳,等.杏李杂交种风味玫瑰引种表现及主要栽培技术[J].山西果树,2011,1:14-15.

    [8] 陈 超,李保国,齐国辉,等.果树自交不亲和机理及应用研究进展. 河北林果研究,2005,20(1):86-90.

    [9] 吴 燕,陈学森,冯建荣,等. 杏杂种一代群体S 基因的遗传研究. 园艺学报,2005,32(4):397-402.

    [10]

    Silva N F, Goring D R. Mechanisms of self-incompatibility in flowering plants[J]. Cellular and Molecular Life Sciences,2001,58: 1988-2007.
    [11]

    Foster E, Levesque-Lemay M, Schneiderman D,et al. Characterization of a gene highly expressed in the Brassica napus pistil that encodes a novel proline-rich protein[J]. Sex Plant Reprod, 2005,17:261-267.
    [12]

    Wang X, Zafian P, Choudhary M,et al. The PR5K receptor protein kinase from Arabidopsis thaliana is structurally related to a family of plant defense proteins[J]. Proceedings of the National Academy of Sciences of the USA, 1996, 93:2598-2602.
    [13]

    Furuyama T, Dzelzkalns V A. A novel calcium-binding protein is expressed in brassica pistils and anthers late in flower development[J]. Plant Molecular Biology,1999,39:729-737.
    [14]

    Fukai E, Nishio T, Nasrallah M. Molecular genetic analysis of the candidate gene for MOD, a locus required for self-incompatibility in Brassica rapa[J]. Molecular Genetics and Genomics,2001,265:519-525.
    [15]

    Sassa H, Hirano H. Style-specific and developmentally regulated accumulation of a glycosylated thaumatin/PR5-like protein in Japanese pear (Pyrus serotina Rehd.)[J]. Planta,1998,205:514-521.
    [16]

    De Graaf B H J, Knuiman B A, Derksen J, et al. Characterization and localization of the transmitting tissue-specific PELPIII proteins of Nocotiana tabacum[J]. Journal of Experimental Botany, 2003, 54(380):55-63.
    [17]

    De Graaf B H J, Knuiman B A, van Der Weerden G M, et al. The PELPIII glycoprotein's in Solanaceae : stylar expression and transfer into pollen tube walls[J]. Sex Plant Reprod,2004,16:245-252.
    [18]

    Expression of an isoflavone reductase-like gene enhanced by pollen tube growth in pistils of Solanum tuberosum[J]. Plant Molecular Biology,1997,33:923-929.
    [19]

    O'Brien M, Bertrand C, Matton D P. Characterization of a fertilization-induced and developmentally regulated plasma- membrane aquaporin expressed in reproductive tissue, in the wild potato Solanum chacoense Bitt[J]. Planta,2002,215: 485-493.
    [20]

    Kuboyama T. A novel thaumatin-like protein gene of tobacco is specifically expressed in the transmitting tissue of stigma and style[J]. Sex Plant Reprod,1998,11:251-256.
    [21]

    Luu D T, Marty-Mazars D, Trick M, et al. Pollen-stigma adhesion in Brassica spp involves SLG and SLR1 glycoproteins[J]. Plant Cell, 1999,11(2):251-262.
    [22]

    Doughty J, Dixon S, Hiscock S J, et al. PCP-A1, a defensin-like Brassica pollen coat protein that binds the S locus glycoprotein, is the product of gametophytic gene expression[J]. Plant Cell, 1998, 10(8):1333-1347.
    [23]

    Samuel M A, Chong Y T, Haasen K E, et al. Cellular pathways regulating responses to compatible and self-incompatible pollen in Brassica and Arabidopsis stigmas intersect at Exo70A1, a putative component of the exocyst complex[J]. Plant Cell,2009,21 (9):2655-2671.
    [24]

    Dai S, Chen T, Chong K, et al. Proteomics identification of differentially expressed associated with pollen germination and tube growth reveals characteristics of germinated Oryza sativa pollen[J]. Mol Cell Proteomics,2007,6 (2):207-230.
    [25]

    Sheoran I S, Pederson E J, Ross A R, et al. Dynamics of protein expression during pollen germination in canola(Brassica napus)[J]. Planta, 2009,230 (4):779-793.
    [26]

    Wang Z, Wan g Z, Shi L,et al. Proteomic alterations of Brassica napus root in response to boron deficiency[J]. Plant Mol Biol,2010, 74(3):265-278.
    [27]

    Yao Y, Sun H, Xu F, et al. Comparative proteome analysis of metabolic changes by low phosphorus stress in two Brassica napus genotypes[J]. Planta, 2011, 233(3):523-537.
    [28] 郑 洲,陈学森,冯宝春,等.杏品种授粉生物学研究[J].果树学报,2004,21(6):327-331.

    [29] 张绍铃,房经贵,杨记磙.果树自交不亲和性的遗传与生理机制及其研究[J].果树学报,2001,18(1):49-52.

    [30]

    Pieton JM, Steer MW. A model for the mechanism of tip extension in pollen tubes [J]. Journal of Theoretical Biology,1982, 98: 15-20.
    [31]

    Pierson ES. Rhodamine-phalloidin staining of F-actin in pollen after dimethysulphoxide permeabilization[J]. Sex Plant Reproduction, 1988,1:83-87.
    [32]

    Lazzaro M D micrdilaments. The spormatogenous body cell of the conifer Picea abies contains actin Protoplasma[J]. 1998, 201:194-201.
    [33] 张 鹏.杨树杂交不亲和性的细胞学及生理生化基础.北京:北京林业大学,2013.

    [34]

    Brewbaker J L, Kwack B H. The essential role of calcium ion in pollen germination and pollen tube growth[J]. Am J Bot, 1963, 50 (9): 859-865.
    [35]

    Rathore K S, Cork R J, Robinson K R. A cytoplasmic gradient of Ca2+ is correlated with the growth of lily pollen tubes[J]. Dev Biol, 1991, (148):612-619.
    [36]

    Gu Y.Q, Vernoud V, Fu Y, et al. ROPgTPase regulation of pollen tubegrowth through the dynamics of tip-localized F-actin[J]. J. Exp. Bot,2003,(54):93-10.
    [37] 尚桂军. TteRRF, AmCBL1初步晶体学和嗜热菌ATCC27502-ATP合酶晶体生长研究.北京:北京林业大学. 2010.

    [38]

    Okuda S, Tsutsui H, Shiina K, et al. Defensin-like polypeptide LUREs are pollen tube attractants secreted from synergid cells[J]. nature, 458 (7236):357-361.
    [39]

    Amien S, Kliwer I, Márton M L, et al. Defensin-like ZmES 4 mediates pollen tube burst in maizevia opening of the potassium channel KZM1[J]. PLoS Biol, 2010, 8 (6):e1000388.
    [40]

    Kessler S A, Shimosato-Asano H, Keinath N F, et al. Conserved molecular components for pollen tube reception and fungal invasion[J]. Science, 2010, 330:968-971.
    [41] 张水军,曾千春,卢秀萍,等.植物富含甘氨酸蛋白的研究进展[J].中国农学通报,2010,26(4):54-58.

    [42] 孙培亮,王志亮,李金明,等.热休克蛋白70研究新进展[J].中国兽医学报,2007,27(2):284-288.

    [43]

    Lehmann S, Funck D, Szabados L,et al. Proline metabo-lism and transport in plant development[J]. Amino Acids, 2010,39:949-962
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Analysis on Differences of Protein in Stylar Canal During Early Pollination Associated with Self-incompatible Prunus domestica × armeniaca ‘Fengweimeigui’

  • 1. Paulownia R&D Center of China, State Forestry Administration, Zhengzhou 450003, Henan, China
  • 2. Non-timber Forestry R&D Center, Chinese Academy of Forestry, Zhengzhou 450003, Henan, China

Abstract: This study focused on the differences of protein in stylar canal between that from self-incompatible Prunus domestica × armeniaca ‘Fengweimeigui’ and that from cross-pollination (ACP) (‘Fengweimeigui’ × ‘Konglongdan’). Two dimension difference gel electrophoresis (2D-DIGE) and mass spectrum (MS) detection techniques were used in this study. The results showed that there were 79 different protein spots in stylar canal between that from self-pollination and that from cross-pollination, and there was a huge difference, more than double, in terms of protein expression among 43 protein spots, including 6 up-expressed and 37 down-expressed in self-pollination stylar canal compared with that in cross-pollination stylar canal. Only 34 protein spots were identified unambiguously through mass spectrometry analysis, including 5 kinds which were catalytic protein, defence/stress protein, and structural protein, respectively.

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