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Citation:

Expression and Functional Analysis of CDPK Gene Family in Populus

  • Received Date: 2013-06-20
  • Calcium-dependent protein kinases (CDPKs) are important Ca2+ sensors and effectors in Ca2+ signal transduction pathway. They play crucial roles in regulation of plant development and tolerance of various environmental stresses. In this study, a genome-wide analysis of Populus CDPKs was performed, including phylogeny, gene structure and expression patterns. Thirty-two CDPK genes and ten CRK genes were identified. Furthermore, the gene expression profiles showed that a number of Populus CDPK differentially expressed across different tissues and developmental stages. Three Populus CDPK gene, i.e. PtCPK1, PtCPK6 and PtCPK15, were cloned and analyzed, revealing their proteins localized in cell membrane. These results may lay the foundation for functional analysis of Populus CDPK gene family in the future.
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Expression and Functional Analysis of CDPK Gene Family in Populus

  • 1. State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

Abstract: Calcium-dependent protein kinases (CDPKs) are important Ca2+ sensors and effectors in Ca2+ signal transduction pathway. They play crucial roles in regulation of plant development and tolerance of various environmental stresses. In this study, a genome-wide analysis of Populus CDPKs was performed, including phylogeny, gene structure and expression patterns. Thirty-two CDPK genes and ten CRK genes were identified. Furthermore, the gene expression profiles showed that a number of Populus CDPK differentially expressed across different tissues and developmental stages. Three Populus CDPK gene, i.e. PtCPK1, PtCPK6 and PtCPK15, were cloned and analyzed, revealing their proteins localized in cell membrane. These results may lay the foundation for functional analysis of Populus CDPK gene family in the future.

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