• 中国中文核心期刊
  • 中国科学引文数据库(CSCD)核心库来源期刊
  • 中国科技论文统计源期刊(CJCR)
  • 第二届国家期刊奖提名奖

Citation:

Pollen Viability and Preservation of Dendrobium lindleyi

  • Received Date: 2012-12-31
  • The pollen of Dendrobium lindleyi was employed as experimental material to investigate the determination method of pollen viability and pollen storage. The results showed that: (1) The optimum culture medium was 100 g·L-1 sucrose + 100 mg·L-1 H3BO3 + 80 mg·L-1 CaCl2. TTC staining was not adapted to detect pollen viability of Den. lindleyi. (2) The pollen germinating rate was over 65% during 0~8 days after blooming. As pollen parents, the male receptivity was over 85% during this period, which showed a better result than bud stage and droop stage. (3) Different storage methods had different effects on pollen viability of Den. lindleyi. The viability of pollen stored at dry condition in 4 ℃ declined to 0 after 80 days. However, after stored at humid condition in 4 ℃, freeze-wetting and freeze-drying in -20 ℃ for 90 days, the pollen viabilities were about 1.4%, 21.7%, and 6.4% respectively. As pollen parents, the pollen freeze-wetting stored in -20 ℃ for 90 days could still set seeds normally, while the setting rate was obviously decreased when using other storage methods.
  • 加载中
  • [1] 王 雁, 李振坚, 彭红明. 石斛兰资源生产应用[M]. 北京:中国林业出版社, 2007:11.

    [2] 吉占和, 陈心启, 罗毅波,等. 中国植物志:第19卷[M]. 北京: 科学出版社, 2006: 78.

    [3]

    Pacini E.Orchid pollen dispersal units and reproductive consequences[C]//Tiiu K, Arditti J, Sek M W. Orchid biology: reviews and perspectives X. Springer-Verlag New York Inc, 2009:185-194.
    [4] 郑宝强, 王 雁, 彭镇华,等.杂种卡特兰花粉萌发和花粉贮藏性研究[J]. 热带亚热带植物学报, 2012,20 (1):13-18.

    [5] 郭丽霞, 莫 饶. 海南原生墨兰花粉萌发试验初报[J]. 热带农业科学, 2007,23 (6) :594-597.

    [6] 胡适宜, 杨 坚. 春兰花粉壁的结构及其与花粉粘合的关系[J]. 植物学报, 1989,31(6):414-421.

    [7]

    Pritchard H W, Prendergast F G. Factors influencing the germination and storage characteristics of orchid pollen [M]//Pritchard H W. Modern Methods in Orchid Conservation: The Role of Physiology, Ecology and Management. Cambridge: Cambridge University Press, 1989: 1-16.
    [8] 律春燕, 王 雁, 朱向涛,等.黄牡丹花粉生活力测定方法的比较研究[J].林业科学研究, 2010,23 (2):272-277.

    [9] 朱惜晨. 芍药花粉生活力测定与杂交亲本选择初步研究[J]. 福建林业科技, 2007, 34(2):121-123.

    [10] 李枝林, 王玉英, 王卜琼. 兰花远缘杂交育种技术研究[J].中国野生植物资源, 2007, 26 (4) : 53-56.

    [11] 李振坚, 亢秀萍, 王 雁. 重唇石斛传粉生物学与显微研究动态[J].西北植物学报, 2009,29 (9):1804-1810.

    [12] 吴应祥. 中国兰花[M].北京:中国林业出版社, 1993: 106-107.

    [13] 欧阳英. 几种兰科花卉的离体保存技术研究[D]. 北京:北京林业大学,2010.

  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article views(3311) PDF downloads(1123) Cited by()

Proportional views

Pollen Viability and Preservation of Dendrobium lindleyi

  • 1. Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China

Abstract: The pollen of Dendrobium lindleyi was employed as experimental material to investigate the determination method of pollen viability and pollen storage. The results showed that: (1) The optimum culture medium was 100 g·L-1 sucrose + 100 mg·L-1 H3BO3 + 80 mg·L-1 CaCl2. TTC staining was not adapted to detect pollen viability of Den. lindleyi. (2) The pollen germinating rate was over 65% during 0~8 days after blooming. As pollen parents, the male receptivity was over 85% during this period, which showed a better result than bud stage and droop stage. (3) Different storage methods had different effects on pollen viability of Den. lindleyi. The viability of pollen stored at dry condition in 4 ℃ declined to 0 after 80 days. However, after stored at humid condition in 4 ℃, freeze-wetting and freeze-drying in -20 ℃ for 90 days, the pollen viabilities were about 1.4%, 21.7%, and 6.4% respectively. As pollen parents, the pollen freeze-wetting stored in -20 ℃ for 90 days could still set seeds normally, while the setting rate was obviously decreased when using other storage methods.

Reference (13)

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return