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The Effects of AtFBDL1 on Apical Meristematic Growth of Arabidopsis thaliana

  • Received Date: 2013-04-09
  • AtFBDL1 is a member of FBD-like gene family, which encodes proteins containing sequences similar to F-box structure domain. Online expression prediction indicates that AtFBDL1 is mainly expressed in shoot apical meristem. Few studies have been conducted on FBD-like genes, which cause the function of these genes remains largely unknown. By using tissue-specific semi-quantitative analysis and GUS staining, the temporospatial expression pattern of AtFBDL1 in Arabidopsis thaliana was investigated. The result showed that in the early stage of seedling development, the AtFBDL1 was mainly expressed in shoot apical meristem and in hypocotyl. After the stage of euphylla emerged, the expression of AtFBDL1 in hypocotyl decreased significantly and mainly restricted the shoot apical meristem. Compared with wild type plants, the AtFBDL1 over-expressed plants displayed slowed growth with up to 12 cm shorter stems, delayed bolting time of 3 to 4 days, reduced rosette leaf area, increased leaf number up to 10, and abnormal leaves. Co-expression analysis showed that the transcription of AtFBDL1 was correlated with several auxin-related or flowering regulation genes. The result of this study indicates that the AtFBDL1 plays an essential role in plant growth and development in Arabidopsis thaliana, especially in the process of the shoot apical meristem differentiation, highly likely through auxin-related pathways.
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The Effects of AtFBDL1 on Apical Meristematic Growth of Arabidopsis thaliana

  • 1. State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
  • 2. College of Horticulture & Forestry Sciences, Huazhong Agricultural University, Wuhan 430000, Hubei, China

Abstract: AtFBDL1 is a member of FBD-like gene family, which encodes proteins containing sequences similar to F-box structure domain. Online expression prediction indicates that AtFBDL1 is mainly expressed in shoot apical meristem. Few studies have been conducted on FBD-like genes, which cause the function of these genes remains largely unknown. By using tissue-specific semi-quantitative analysis and GUS staining, the temporospatial expression pattern of AtFBDL1 in Arabidopsis thaliana was investigated. The result showed that in the early stage of seedling development, the AtFBDL1 was mainly expressed in shoot apical meristem and in hypocotyl. After the stage of euphylla emerged, the expression of AtFBDL1 in hypocotyl decreased significantly and mainly restricted the shoot apical meristem. Compared with wild type plants, the AtFBDL1 over-expressed plants displayed slowed growth with up to 12 cm shorter stems, delayed bolting time of 3 to 4 days, reduced rosette leaf area, increased leaf number up to 10, and abnormal leaves. Co-expression analysis showed that the transcription of AtFBDL1 was correlated with several auxin-related or flowering regulation genes. The result of this study indicates that the AtFBDL1 plays an essential role in plant growth and development in Arabidopsis thaliana, especially in the process of the shoot apical meristem differentiation, highly likely through auxin-related pathways.

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