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Citation:

Identification of Rosa sterilis Based on AFLP Molecular Markers and DNA Barcodes

  • Received Date: 2014-07-21
  • Nineteen Rosa roxbunghii, R. sterilis and R. sterilis var. leioclada specimen collected from Xing'ren and Anshun in Guizhou of China were identified by AFLP molecular markers and DNA barcodes. The results of AFLP showed that genetic similarity coefficient among the 19 specimen ranged from 0.719 0 0.997 2 and the average was 0.936 5. The result of UPGMA cluster analysis showed that the 19 specimen could be divided into two groups. R. roxbunghii was distinct from the others and formed one group separately, which the others fall into another group. The result of DNA barcodes showed that there was not variable site in ITS sequences and 4 cpDNA sequences showed highly closed affinity. Based on combined sequences, the average error was 0.000 6, while the genetic distance between R. roxbunghii and the others was 0.005 8. The identification results of two methods were consistent. R. sterilis and R. roxbunghii were two segregated species. The R. sterilis collected from Xing'ren and R. sterilis var. leioclada collected from Anshun belong to the same species. The results of this study determined the phylogeny status of R. sterilis at the level of molecular and gene and laid the theoretical foundation for further collection, preservation, exploitation and application of R. sterilis germplasm resources.
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Identification of Rosa sterilis Based on AFLP Molecular Markers and DNA Barcodes

  • 1. Southwest Forestry University, Kunming 650224, Yunnan, China
  • 2. Yunnan Academy of Biodiversity, Kunming 650224, Yunnan, China
  • 3. Rocky Desertification Comprehensive Treatment Office of Xing'ren County, Xing'ren 562300, Guizhou, China

Abstract: Nineteen Rosa roxbunghii, R. sterilis and R. sterilis var. leioclada specimen collected from Xing'ren and Anshun in Guizhou of China were identified by AFLP molecular markers and DNA barcodes. The results of AFLP showed that genetic similarity coefficient among the 19 specimen ranged from 0.719 0 0.997 2 and the average was 0.936 5. The result of UPGMA cluster analysis showed that the 19 specimen could be divided into two groups. R. roxbunghii was distinct from the others and formed one group separately, which the others fall into another group. The result of DNA barcodes showed that there was not variable site in ITS sequences and 4 cpDNA sequences showed highly closed affinity. Based on combined sequences, the average error was 0.000 6, while the genetic distance between R. roxbunghii and the others was 0.005 8. The identification results of two methods were consistent. R. sterilis and R. roxbunghii were two segregated species. The R. sterilis collected from Xing'ren and R. sterilis var. leioclada collected from Anshun belong to the same species. The results of this study determined the phylogeny status of R. sterilis at the level of molecular and gene and laid the theoretical foundation for further collection, preservation, exploitation and application of R. sterilis germplasm resources.

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