• 中国中文核心期刊
  • 中国科学引文数据库(CSCD)核心库来源期刊
  • 中国科技论文统计源期刊(CJCR)
  • 第二届国家期刊奖提名奖

Citation:

Advances in Tissue Culture Techniques of Trees and the Problems Existed

  • Received Date: 2015-04-16
  • The advances in forest plant tissue culture in recent years are reviewed from the aspects of explant selection, explant differentiation pathway, and the factors that have effects on proliferation and rooting in the process of tissue culture. There are three differentiation pathways (axillary bud germination, indirect organogenesis and somatic enbryogenesis), in which the axillary bud germination is popularity. It is necessary to choose different kind of explant according to the differentiation pathway. The culture medium and plant growth regulator are the main factors influencing the proliferate. The study on culture medium has shifted from the selection of general medium to culture medium without sucrose, and the concepts of photoautotrophic culture and open tissue culture emerged. Plant growth regulator is the critical factor for rooting. The interaction of endogenous hormone and exogenous hormones has great influence on proliferation. The causes and solutions of vitrification, browning and pollution in tissue culture were explained. Studying on vitrification and browning focused on the physiological state and culture environment of explant. It is suggested that tissue culture without sucrose could less the vitrification and browning by improvement of culture environment. Traditional culture focuses on solving the pollution problem by aseptic technique, resulting in high cost. The new tissue culture could lower the requirement of aseptic technique by adding bacteriostats and taking out sucrose. Open culture without high temperature and pressure sterilization under the adding of bacteriostats. Photoautotrophic culture which making use of the photosynthetic capacity of cultured explants, reducing the concentration of sucrose, and adjusting the light conditions, concentration of CO2, and moisture is another method for tissue culture. Both focus on reducing cost and simplifying culture procedure.
  • 加载中
  • [1] 李盟, 高亦珂. 植物组织培养新技术研究进展[J]. 广东农业科学, 2009(3):152-154.

    [2] 施琼, 胡峰, 黄烈健, 等. 马大杂种相思腋芽高效组培增殖体系[J]. 林业科学, 2014, 50(6):55-60.

    [3] 胡峰, 施琼, 黄烈健. 马占相思和大叶相思优树组培不定根诱导研究[J]. 南京林业大学学报:自然科学版, 2015, 39(2):57-62.

    [4] 胡峰, 施琼, 黄烈健. 厚荚相思(Acacia crassicarpa)腋芽组培快繁技术体系研究[J]. 植物研究, 2015, 35(2):179-184.

    [5] 施琼, 胡峰, 黄烈健, 等. 马大杂种相思组培快繁技术[J]. 华南农业大学学报, 2015, 36(2):79-84.

    [6]

    Kozai T, Iwanami Y. Effects of CO2 enrichment and sucrose concentration under high photon fluxes on plantlet growth of Carnation[J]. Japan Soc Hort Sci, 1988, 57(2):279-278.
    [7]

    Kozai T, Koyoma Y, Watanabe I. Multip lication of potato plantlets in vitro with sugar-free medium under high photosynthetic photon flux[J]. Acta Horticulturae, 1988, 230:121-127.
    [8] 周炜, 曲英华. 无糖组培技术在我国的研究进展[J]. 农村实用工程技术(温室园艺), 2005(7):24-25.

    [9] 卢加举, ArakTira-Umphon, 张正学, 等. "黔兴1号"抑菌剂在甘蔗开放组培中的影晌[J]. 农业科学与技术(英文版), 2014, 15(9):1478-1481.

    [10] 陈英, 张西英, 刘江娜. SDIC在马铃薯脱毒组培苗开放式快繁生产中的应用试验研究[J]. 新疆农垦科技, 2014, 37(11):38-40.

    [11] 刘福平. 植物抗菌组培中抗菌剂应用的研究方法[J]. 基因组学与应用生物学, 2014, 33(4):910-915.

    [12] 刘丽丽. 东北红豆杉开放式组培育苗关键技术研究[D]. 吉林:吉林大学, 2013:1-91.

    [13] 丁国昌, 高淑慧, 林思祖, 等. 黑木相思开放式光自养微繁殖的研究[J]. 福建林学院学报, 2010, 30(1):11-14.

    [14] 管道平, 刘文科, 杨其长, 等. 植物光自养培养箱CO2自动调控系统的设计与试验[J]. 林业科学, 2007, 43(5):116-119.

    [15] 占爱瑶, 詹亚光. 植物组织培养新技术:光自养微繁[J]. 生物技术通报,2007(4):85-89.

    [16] 王玫瑞, 刘孟军, 代丽. 枣树组织培养研究进展[J]. 果树学报, 2002, 19(5):336-339.

    [17] 刘会超, 郭丽娟, 贾文庆, 等. 月季组织培养研究进展[J]. 河南科技学院学报:自然科学版, 2007, 35(3)45-47.

    [18] 汪长水. 卷荚相思组培快繁技术研究[J]. 福建林业科技, 2009, 36(3):92-97.

    [19]

    Adela H, Ina P. Plant regeneration from Rosa shoot tips cryop reserved by a combined droplet vitrification method[J]. Plant Cell, Tissue and Organ culture, 2006, 84(2):100129-100137.
    [20] 赖家业, 周传明, 叶春生, 等. 厚荚相思组织培养与快速繁殖[J]. 四川大学学报:自然科学版, 2003, 40(5):982-985.

    [21] 张玫瑰. 杉木优良无性系组织培养及再生体系研究[D]. 福建:福建农林大学, 2013:1-63.

    [22] 罗君琴, 徐建国, 王平. 不同培养条件对柑橘胚性愈伤组织离体诱导的影响[J]. 北方园艺, 2013, 37(13):125-127.

    [23] 刘振林, 潘玉霞, 姬玉, 等. 百花怪柳的组培与快繁技术[J]. 技术开发, 2013, 27(5):11-114.

    [24]

    Amutha S, Ganapathi A, Muruganantham M. In vitro organogenesis and plant formation in Vigna radiate (L.) Wilczek[J]. Plant Cell, Tissue Organ Culture, 2003, 72(2):203-207.
    [25] 林娅, 郑玉, 刘青林. 影响月季愈伤组织诱导和分化的因素[J]. 分子植物育种, 2006, 4(2):223-227.

    [26] 韩登媛, 李旦, 赵健, 等. 华北落叶松胚性愈伤组织诱导影响因子的研究[J]. 林业科学研究, 2013, 26(4):454-458.

    [27] 陈志, 陈金慧, 边黎明, 等. 杂交鹅掌楸胚性细胞悬浮系的建立[J]. 分子植物育种, 2007, 5(1):137-140.

    [28] 李惠华. 龙眼体胚发生过程中激素代谢和信号转导相关基因的克隆与表达[D]. 福建:福建农林大学, 2011:1-223..

    [29] 胡恒康, 江香梅, 张启香, 等. 碳源对山核桃体细胞胚发生和植株再生的影响[J]. 浙江农林大学学报, 2011, 28(6):911-917..

    [30] 汤浩茹, 王永清, 任正隆. 核桃体细胞胚发生与转基因研究进展[J]. 林业科学, 2000, 36(3):102-110.

    [31] 邹晖, 陈永快, 王伟英. 香樟组培快繁技术研究进展[J]. 福建农业科技, 2009(1):71-72.

    [32] 陈桂芳, 娄利华. 银荆相思组织培养及快繁技术研究[J]. 西南农业大学学报:自然科学版, 2004, 26(2):195-197.

    [33] 张祖荣, 刘兴良. 灰木相思茎段腋芽的组织培养及植株再生[J]. 西南农业大学学报:自然科学版, 2004, 26(3):291-293.

    [34] 徐刚, 牟豪杰, 汪一婷. 橡皮树组培及成品苗养护[J]. 中国花卉园艺, 2009(4):20-21.

    [35] 盛丽莉, 史文亚, 张志敏. 青钱柳组织培养技术研究进展[J]. 西南林业大学学报, 2011, 31(2):84-89.

    [36] 高淑慧. 厚英相思和黑木相思光自养微繁殖技术的研究[D]. 福建:福建农林大学, 2007:1-46.

    [37] 马凤桐, 刘玉荣. 成龄桑树冬芽的组织培养[J]. 植物生理学通讯, 1985, 9(1):34-35.

    [38]

    Veneta K T, Hendrik J V T, Elena Y. Role of phenylurea cytokine in CPPU in apical dominance release in In vitro cultured rosa hybridal[J]. Journal of Plant Growth Regulation, 2000, 19(2):232-237.
    [39]

    Niels B, Kell K. Changes in concentrations of cytok in ins (CKs) in root and axillary bud tissue of miniature rose suggest that local CK biosynthesis and zeatin-type CKs play important roles in axillary bud growth[J]. Journal of Plant Growth Regulation, 2005, 24(3):238-250.
    [40] 牙祖韧, 韦鹏宵, 芩秀芬, 等. 不同激素处理对厚荚相思组培苗生根及移栽效应[J]. 广西林业科学, 2007, 36(1):53-64.

    [41] 陈凌艳, 郑宇, 陈礼光,等. 西洋杜鹃组培苗生根培养及其内源激素含量变化的研究[J].福建林学院学报, 2011, 31(2):131-135.

    [42] 高红宾, 王朋飞, 刁绍启, 等. 6-BA对酸樱桃组培苗4种内源激素质量分数动态变化的影响[J]. 东北林业大学学报, 2007, 35(7):46-48.

    [43] 李代丽, 康向阳. 植物愈伤组织培养中内外源激素效应的研究现状与展望[J]. 生物技术通讯, 2007, 18(3):546-548.

    [44] 黄烈健, 陈祖旭, 张赛群. 马占相思优树组培快繁技术研究[J]. 林业科学研究, 2012, 25(2):227-230.

    [45] 谢志亮, 吴振旺. 木本植物组培褐化研究进展[J]. 中国南方果树, 2013, 42(5):42-46.

    [46] 马莉贞. 植物组织培养中褐变现象的研究[J]. 安徽农业科学, 2006, 34(15):3583-3584.

    [47]

    Gannoun S, Lionakis S M, Gemsopoulos D, et al. Aspects of in vitro culture of Pistacia terebinthus and Pistacia vera[J]. Acta Horticulturae, 1995, 419:201-206.
    [48] 邹英宁, 李国怀, 吴强盛. 中国李组织培养过程中褐变的抑制研究[J].山地农业生物学报, 2007, 26(6):508-512.

    [49] 李冬杰, 张进献, 魏景芳, 等. 培养基和培养条件与红豆杉细胞培养中褐化的关系[J]. 植物生理学通讯, 2005, 41(1):95-98.

    [50] 刘杰, 张玉星, 董祯. 梨组培褐化及抗褐措施研究进展[J]. 河北林果研究, 2008, 23(2):195-199.

    [51] 黄燕芬, 周国兰, 赵华富. 降低茶树组织培养中外植体褐化程度的研究[J]. 西南农业学报, 2009, 22(5):1492-1495.

    [52] 邱璐, 陈善娜, 夏跃明, 等. 桑树组织培养中褐化问题的研究[J]. 云南大学学报:自然科学版, 2000, 22(1):76-78.

    [53] 毛红俊, 孔祥生, 张妙霞. 香水白掌离体培养褐化反应的初步研究[J]. 北方园艺, 2010, 34(10):182-184.

    [54] 陈蕾, 曹后男, 宗成文, 等. 降低苹果梨组培过程中外植体褐化的研究[J]. 北方园艺, 2008, 32(10):139-142.

    [55] 张振霞, 郑玉忠. 番荔枝组培中的褐化及防止措施研究[J]. 中国南方果树, 2009, 38(3):41-42.

    [56] 王小敏, 吴文龙, 李海燕, 等. 黑莓外植体褐化影响因素分析及适宜培养条件筛选[J].植物资源与环境学报, 2009, 18(3):63-68.

    [57] 张明文, 陈力耕. 银杏组织培养中控制褐化的研究[J]. 中国南方果树, 2003, 32(3):51-52.

    [58] 陈斌, 杨娟. 红豆杉细胞继代培养防褐变措施的研究[J]. 天然产物研究与开发, 2001, 13(4):8-11.

    [59] 王东霞, 李长杰. 如何对抗植物组培中的组织褐变[J]. 中国花卉盆景, 2002, 29(2):17.

    [60] 盛长忠, 王淑芳, 王宁宁, 等. 红豆杉愈伤组织培养中褐变现象的初探[J]. 南开大学学报,2001, 34(4):120-122.

    [61] 闫桂琴, 张伟, 张艳芳. 翅果油树脱毒试管苗的组织培养技术研究[J]. 西北植物学报, 2003, 23(7):1297-1303.

    [62] 熊丽, 吴丽芳. 观赏花卉的组织培养与大规模生产[M]. 北京:化学工业出版社, 2003.

    [63] 刘洋, 苏淑钗, 冷平生, 等. 阿月浑子外植体褐变抑制方法[J]. 果树学报, 2007, 24(3):393-396.

    [64] 张宏平, 姬爱国, 和林涛. 植物组培快繁褐化现象研究进展[J]. 农业工程, 2013, 3(5):128-130.

    [65] 陈兵先, 黄宝灵, 吕成群, 等. 植物组织培养试管苗玻璃化现象研究进展[J]. 林业科技开发, 2011, 25(1):1-5.

    [66] 蔡祖国, 徐小彪, 周会萍. 植物组织培养中的玻璃化现象及其预防[J]. 生物技术通讯, 2005, 16(3):353-355.

    [67]

    Phan C T, Hegedus P. Possible metabolic basis for the development anomaly observed in vitro culture, called ‘vitreous plants’[J]. Plant Cell Tissue Org Cult, 1986, 6(1):83-94.
    [68] 卢兴霞, 柴慈江, 张婷, 等. 栒子试管苗玻璃化影响因素的研究[J]. 北方园艺, 2014, 38(18):103-106.

    [69] 邵龙珠, 赵淑君, 王淑荣, 等. 植物组织培养中的常见问题与解决技术措施[J]. 林业勘查设计, 2012(1):49-51.

    [70] 黄海波, 淡明. 植物组织培养中存在的主要问题与对策[J]. 安徽农业科学, 2006, 34(12):2632-2633.

    [71] 巩健. 植物组织培养快繁中存在的主要问题及防止措施[J]. 科技信息, 2008(3):214-215.

    [72] 张薪薪, 唐金花, 王关林. 抑菌剂在开放组培中的使用及效果研究[J]. 辽宁师范大学学报:自然科学版, 2005, 28(4):466-469.

    [73] 陈本学. 珍珠相思光自养微繁殖技术再生体系的建立[D]. 福建:福建农林大学, 2008:1-45.

    [74]

    Nhut D T, Takamura T, Watanabe H, et al. Artificial light source using light-emitting diodes (LEDs) in the efficient micropropagation of Spathiphyllum plantlets[J]. Acta Horticulturae (ISHS), 2005, 692:137-142.
    [75] 牟宁宁, 高亦珂. 植物无糖组培技术研究进展[J]. 林业科技开发, 2007, 21(1):10-12.

    [76] 崔刚, 单文修, 秦旭, 等. 植物开放式组织培养研究初探[J]. 山东农业大学学报:自然科学版, 2004, 35(4):529-533.

    [77] 王家庆, 李晓燕. 植物组培新方向-开放组培与无糖暴露组培研究概况[J]. 辽宁农业科学, 2007(1):44-45.

    [78] 陈本学, 林思祖, 曹光球. 观赏多花相思光自养生根培养研究[J]. 北方园艺, 2012, 36(23):71-75.

    [79] 仇金维. 杉木优良无性系光自养微繁殖技术[D]. 福建:福建农林大学, 2012:1-87.

    [80] 徐景云. 不同光自养条件下根际微环境对桉树(Eucalyptus sp.)组培不定芽生根的影响研究[D]. 福建:福建农林大学, 2010:1-50.

    [81] 丁昌国, 林思祖, 陈宇, 等. 基质径级和NPK水平对马占相思光自养幼苗的影响[J]. 福建农林大学学报:自然科学版, 2012, 41(3):238-242.

  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article views(3755) PDF downloads(1608) Cited by()

Proportional views

Advances in Tissue Culture Techniques of Trees and the Problems Existed

  • 1. Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, Guangdong, China

Abstract: The advances in forest plant tissue culture in recent years are reviewed from the aspects of explant selection, explant differentiation pathway, and the factors that have effects on proliferation and rooting in the process of tissue culture. There are three differentiation pathways (axillary bud germination, indirect organogenesis and somatic enbryogenesis), in which the axillary bud germination is popularity. It is necessary to choose different kind of explant according to the differentiation pathway. The culture medium and plant growth regulator are the main factors influencing the proliferate. The study on culture medium has shifted from the selection of general medium to culture medium without sucrose, and the concepts of photoautotrophic culture and open tissue culture emerged. Plant growth regulator is the critical factor for rooting. The interaction of endogenous hormone and exogenous hormones has great influence on proliferation. The causes and solutions of vitrification, browning and pollution in tissue culture were explained. Studying on vitrification and browning focused on the physiological state and culture environment of explant. It is suggested that tissue culture without sucrose could less the vitrification and browning by improvement of culture environment. Traditional culture focuses on solving the pollution problem by aseptic technique, resulting in high cost. The new tissue culture could lower the requirement of aseptic technique by adding bacteriostats and taking out sucrose. Open culture without high temperature and pressure sterilization under the adding of bacteriostats. Photoautotrophic culture which making use of the photosynthetic capacity of cultured explants, reducing the concentration of sucrose, and adjusting the light conditions, concentration of CO2, and moisture is another method for tissue culture. Both focus on reducing cost and simplifying culture procedure.

Reference (81)

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return