• 中国中文核心期刊
  • 中国科学引文数据库(CSCD)核心库来源期刊
  • 中国科技论文统计源期刊(CJCR)
  • 第二届国家期刊奖提名奖

Journal Introduction

Forestry Science Research is a comprehensive academic journal of forestry science sponsored by the Chinese Academy of Forestry Science. The main task is to timely reflect the latest research results, academic papers and reports, scientific and technological trends and information of forestry science with the Chinese Academy of Forestry as the main body, to promote academic exchanges at home and abroad, to carry out academic discussions, to prosper forestry science and to better serve China's forestry construction. The main contents are: forest seeds, seedling raising and afforestation, forest plants, forest genetic breeding, tree physiology and biochemistry, forest insects, resource insects, forest pathology, forest microorganisms, forest birds and animals, forest soil, forest...

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Variation Characteristics of Secondary Vascular Bundles in Pinus massoniana
YANG Chao, FAN Fu-hua, XU Gang
[Abstract](0) [FullText HTML](0) [PDF 3115KB](0)
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Objective To reveal the changes of cellular structure, composition and function of secondary vascular tissue in Pinus massoniana during development. Methods The change of morphology, structure, lignin and cellulose in the vascular tissue of P. massoniana were studied by histochemical analysis and cell segregation. Results The vascular cambium gradually enriched cellulose when it differentiated into new phloem and xylem. During the development of phloem, the sieve cells were deformed and lignified, and the proportion of cellulose in phloem decreased. During the development of xylem tracheids from early wood to late wood, the diameter of striated pores decreased, the number of striated pores decreased, the lignification degree of secondary wall increased, the cell lumen area decreased, the proportion of cellulose content decreased, and the choroidal and radial walls thickened. Conclusion The cellular composition and structure of secondary vascular tissues change regularly with development. The intracellular and intercellular transport capacity of xylem cells decrease, while the mechanical strength increases. The secondary phloem lost its ability to transport and its mechanical strength increases.
Effects of Human Intervention on Soil Physical and Chemical Properties of Walnut Forest on Taihang Hillside
LI Xin, LIU Qian-yuan, GUO Wen-fang, WANG Jia-le, LIU Ya-nan, Li Han-cong, Chen Yan-mei
[Abstract](29) [FullText HTML](26) [PDF 717KB](1)
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Objective To explore the impact of human intervention on the physical and chemical properties of the soil of artificial economic forest on mountain slopes, and to provide scientific basis for forest soil restoration and sustainable management. Methods Based on the walnut forests with three intervention methods: unmanaged, clearing of shrubs and grasses, and clearing of shrubs and grasses and fertilizing on Taihang Mountains, the effects of the three interventions on the soil physical and chemical properties of the walnut forest were compared and analyzed through field investigation and laboratory experiments, and principal component analysis was used for comprehensive evaluation of soil quality. Results (1) The physical and chemical properties of soil in walnut forests with different human intervention were significantly different. Soil clay, silt, electrical conductivity, organic carbon, total nitrogen and total phosphorus in the unmanaged forests were the highest, while soil sand and water content were the lowest; The soil clay, silt, electrical conductivity, organic carbon and total nitrogen in the clearing shrubs and grasses were significantly lower than those in the unmanaged forests (p<0.05), while the soil sand and water content were significantly higher than those in the unmanaged forests (p<0.05); The soil clay, silt, conductivity, water content, total nitrogen, total phosphorus and total potassium in the cleared shrub and grass for fertilization were slightly higher than those in the cleared shrub and grass, and the soil sand, pH and organic carbon were slightly lower than those in the cleared shrub and grass. (2) There was a significant correlation between the physical and chemical properties of walnut forest soil in each intervention. There was a very significant negative correlation between soil clay and sand (p<0.01), a significant (p<0.05) or very significant (p<0.01) positive correlation between soil organic carbon and total nitrogen; and the other soil physical and chemical indicators showed different degrees of correlation changes in the internal soil layers of each intervention and drying. (3) There was a certain degree of variation in the physical and chemical properties of walnut forest soil under each intervention. The soil pH showed weak variability, the soil organic carbon and total nitrogen showed medium variability, and the rest of the soil physical and chemical indicators were in the dynamic changes of weak variability and medium variability between the soil layers within each intervention and intervention. (4) soil quality in the unmanaged forests was the best (2.120), followed by> clean shrub and grass fertilization (−0.820) and clean shrub and grass (−1.258)The soil quality in clean shrub and grass fertilization walnut forest was not improved significantly. Conclusion Human intervention significantly affects the physical and chemical properties of soil in walnut forest on Taihang hillside and causes differences in soil quality. Therefore, the regulation and management of human intervention should be paid attention to in the management of walnut forest.
The Variation of Stand Structure with Age and Its Hydrological Effects of Larch Plantation in Liupan Mountains
XU Xiao-qin, YU Peng-tao, WANG Yan-hui, XU Li-hong, YU Yi-peng, LIU Ze-bin
Accepted Manuscript
[Abstract](803) [FullText HTML](498) [PDF 867KB](18)
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Objective To study the change of stand structure with age and its hydrological influence, and provide a theoretical basis for forest vegetation construction and management in the semi-arid area, Liupan Mountains. Method The main plantation type -- Larix principis-rupprechtii (Larch) plantation was selected in Diediediegou small watershed in the semi-arid area, Liupan Mountains. Based on daily meteorological data from 1993—2002, both the coupled stand structure model and BROOK90, an ecohydrological model were applied to simulate the variation of stand structures (average tree height, diameter at breast height (DBH), canopy density and leaf area index (LAI)) with plantation age, and then its effects on each water balance component (interception, transpiration, soil evaporation and runoff) were obtained. Result The Larch plantation could be divided into rapid growth period (5~15 years old), slow growth period (15~40 years old) and stable period (over 40 years old) with the increase of age. During the rapid growth period, the average tree height, average DBH, canopy density and canopy LAI increased rapidly with the increase of age, with an average annual growth rate of 0.48 m·a−1, 0.54 cm·a−1, 0.03 and 0.19, respectively. During the slow growth period, the average stand tree height, DBH and canopy density increased slowly, while the LAI increased first and then decreased. But during the stable period, the stand structures did not change significantly. In the fast growth period, the average annual interception and transpiration increased rapidly with the increase of age, and the rate was 1.91 and 24.13 mm·a−1, respectively. Meanwhile, the average annual soil evaporation and water yield decreased rapidly with the increase of age, and the decrease rate was 10.58 and 14.88 mm·a−1, respectively. In the slow growth period, the change of average annual transpiration, soil evaporation and runoff with age slowed down, and tended to be stable when the age was 30 years. The average annual change rates were 0.62, −0.75 and −0.76 mm·a−1, respectively. Conclusion In the semi-arid area of Liupan Mountains, the stand structure of Larch plantations changes continuously when the stand age is less than 30 years, especially less than 15 years, which significantly affectes the eco-hydrological function. And when the stand age is over 30 years old, the stand structure is stable and the water balance components tend to be stable too, which indicates that it is no longer necessary to consider the influence of stand age in the integrated forest-water management.
Transcriptome Analysis and Gene Discovery of Abscisic Acid Signaling Pathway in Kandelia obovata under Low Temperature Stress
GUO Jin-min, YANG Sheng, LIU Xing, WANG Jin-wang, WANG Wen-qing, CHEN Qiu-xia
[Abstract](45) [FullText HTML](38) [PDF 1391KB](2)
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Objective To understand the molecular mechanism of Kandelia obovata in response to low temperature stress and cultivate new varieties of cold resistance. Method Based on the annual container seedlings of 'Longgang' K. obovata, a cold-tolerant mangrove cultiva, the control group ( CK ) was treated at 15 oC for 12 h and the low temperature group ( LT ) was treated at −5 oC for 12 h. Illumina HiSeq sequencing platform was used for transcriptome sequencing, and the genes related to abscisic acid signaling pathway were discovered. Result The results showed that a total of 148 transcription factors were identified by transcriptome sequencing, which belonged to 25 transcription factor families. Among them, ERF, NAC, WRKY, bHLH, MYB, bZIP, HB-other and MYB-related families contained more genes, which were 17, 14, 12, 12, 10, 9, 6 and 6, respectively. A total of 1 330 differentially expressed genes ( DEGs ) were screened in the differential group, of which 698 ( 52.48% ) were up-regulated and 632 ( 47.52% ) were down-regulated. KEGG pathway enrichment analysis showed that DEGs were significantly enriched in plant hormone signal transduction, phenylpropanoid biosynthesis, galactose metabolism, photosynthesis-antenna protein, and alpha-Linolenic acid metabolism. Among the abscisic acid signaling pathways, KoPYL1, KoABF1, and KoABF2 were up-regulated and KoPP2C1 and KoABF3 were down-regulated, and the expression of these genes was consistent with the qRT-PCR validation results. Conclusion ERF, NAC, WRKY, bHLH, MYB, bZIP, HB-other and MYB-related play an important role in regulating the response of K. obovata to low temperature stress. Phytohormone signal transduction, phenylpropanoid biosynthesis, galactose metabolism, photosynthesis-antenna protein and alpha-Linolenic acid metabolism are important KEGG pathways in K. obovata in response to low temperature stress. KoPYL1, KoPP2C1, KoABF1, KoABF2 and KoABF3 genes in abscisic acid signaling pathway can be used as important candidate genes for future studies on the response of K. obovata to cold stress.
Relationship between Ammonia-Oxidizing Archaea Community Structure and Nitrate Nitrogen Content in Chinese Fir Plantations at Different Generations
CHEN Wen-wen, WANG Shu-zhen, JIANG Yu-jie, ZHOU Chui-fan
[Abstract](42) [FullText HTML](23) [PDF 1748KB](1)
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Objective This article aims to analyze the changes of soil available nitrogen content in Chinese fir plantations at different generations, and explore the internal relationship between nitrate nitrogen and the structure and diversity of ammonia-oxidizing archaea community, which provides reference for the effective use of soil nitrogen of plantations and soil quality assessment of Chinese fir forest. Method Four Chinese fir plantations with different generations were selected in Nanping, Fujian Province. High-throughput sequencing technology was used to determine the amoA gene amplified by PCR. Mantel_r correlation analysis, random forest model and partial least squares path model were used to study the relationship between soil available nitrogen content, ammonia-oxidizing archaea community abundance and diversity in different generations. Result The content of nitrate nitrogen (NO3-N), microbial biomass nitrogen (MBN), and the enzyme activity significantly decreased with the increase of continuous cropping generations of Chinese fir. The abundance and diversity of ammonia-oxidizing archaea (AOA) community showed a decreasing trend. Soil available nitrogen content was closely related to ammonia-oxidizing archaea community and enzyme activity, and the abundance and diversity of ammonia oxidizing archaea community was the most important factor affecting nitrate nitrogen content. Conclusion With the increase of planting generation, the abundance and diversity of ammonia oxidizing archaea community decrease to a certain extent. Except for the fourth generation of soil urease and ammonia nitrogen oxygenase enzyme activity increased slightly, soil nitrogen cycle related enzyme activity shows a decreasing trend, resulting in a significant reduction in soil nitrate nitrogen content, which limits the effectiveness of soil nitrogen in continuous cropping Chinese fir plantations.
Effects of 8 Host Young Trees on Growth, Development and Nutritional Efficiency of Hyphantria cunea Larvae
Zhang Shu-man, Fang Jing, Zhao Jia-qi, Wang Kai, Ren Ying-jie, Meng Zhao-jun, Yan Shan-chun
[Abstract](103) [FullText HTML](77) [PDF 1447KB](4)
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Objects To explore the adaptability of Hyphantria cunea to different host young trees, scientifically protect trees and prevent and control Hyphantria cunea. Methods Based on eight species of trees widely distributed and planted in Northeast China (Ulmus pumila, Juglans mandshurica, Salix matsudana, Betula platyphylla, Fraxinus mandshurica, Larix olgensis, Albizia kalkora, Tilia amurensis), the growth, development and nutritional effects of H. cunea larvae fed on leaves of different host young trees were measured. Results The survival rate of H. cunea larvae fed on U. pumila and J. mandshurica was the highest, followed by F. mandshurica, S. matsudana and T. amurensis; Feeding on L. olgensis was lower than A. kalkora. The larvae of H. cunea feeding on U. pumila and J. mandshurica had the highest values of weight, body length and head shell width, followed by F. mandshurica and T. amurensis; The feeding rate of A. kalkora was lower than B. platyphylla. The pupation rate and eclosion rate of H. cunea larvae fed on J. mandshurica, U. pumila and F. mandshurica were higher, and the development period was shorter, than T. amurensis; Feeding L. olgensis and A. kalkora could not pupate, and feeding B. platyphylla, A. kalkora and L. olgensis could not eclosion and had a long development period. H. cunea larvae fed the most on U. pumila, J. mandshurica and F. mandshurica, followed by T. amurensis and S. matsudana; The food utilization rate and food conversion rate of larvae feeding on U. pumila and J. mandshurica were at a high level, followed by T. amurensis and F. mandshurica; The larvae fed the lowest amount of A. kalkora and B. platyphylla, and their food utilization rate and food conversion rate were at the lowest level; The results of larval food consumption rate were contrary to their food utilization rate and food conversion rate. Conclusion U. pumila and J. mandshurica are more suitable for the growth and development of H. cunea larvae, followed by F. mandshurica and T. amurensis; However, L. olgensis and A. kalkora are not suitable for their growth; The results show that the H. cunea has different adaptability and nutritional utilization strategies to a variety of hosts.
The Gall Wasps on Common Fagaceae and Three New Record Species from China
PAN Ke-yu, WU Jian-jun, LONG Cheng-peng, QU Ai-jun, WANG Yi-ping
[Abstract](93) [FullText HTML](75) [PDF 3718KB](5)
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Objective To understand the gall wasps on Fagaceae plants in China for providing the basis of collection rapid identification and forecast. Method We have conducted an investigation on 9 common Fagaceae with field survey, collecting and lab rearing. Result The result showed there were 15 species of the gall wasps belonging to 8 genera, including 03 new record species in China and 12 known speices. Conclusion The host, distribution range and gall characteristics of various species have been recorded in this paper. The photos of gall characteristics of 12 species have been provided, and the host plants of most gall wasps have been identified.
Analysis of Tannin of Chinese Gallnut by UHPLC– QTOF–MS Analysis of Tannin of Galla Chinensis by HPLC– QTOF–MS
NI Bing-bing, LIU Hong, YU Li-yang, ZHANG Guo-yun, HE Cai-yun, ZHANG Jian-guo
[Abstract](131) [FullText HTML](70) [PDF 1429KB](1)
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Objective To develop an effective method for characterizing tannin of Chinese gallnut by ultra-performance liquid chromatography–quadrupole time of flight mass spectrometry (UHPLC–QTOF–MS), and analyze the fragmentation behavior of gallotannins. Methods High temperature and ultrasonic–assisted methods were implemented for extracting phenolic compounds from Chinese gallnut, and the fragmentation pathway of gollotannins were characterized by MS/MS spectrometry. Results A total of 20 compounds were identified, including 14 gallotannins, 2 gallagyl esters, 3 phenolic acids, and 1 flavanol. Gallotannins with molecular mass higher than 1500 Da were not identified when high temperature 121 ℃ was used as extract method, while they were successfully detected by the ultrasound extract method, including 1–O–galloyl–βD–glucose to 14–O–galloyl–βD–glucose. Under the conditions of ultrasonic power of 1 500 W and extraction temperature of 65 ℃, more gallotannin isomers were identified. The MS/MS spectrum showed that gallotannins were mainly decomposed by the loss of glucoside, gallic acid, galloyl, H2O and carboxyl. Conclusion In the study, UHPLC–QTOF–MS technology coupled with ultrasonic extraction methods have been proved to determine gallnut tannin comprehensively and efficiently. The identification of gallnut tannin and the analysis of their fragmentation mechanism can provide a reference for research on the chemical composition of other plants rich in tannin.
Selection of High Efficient Fungi for Litter Degradation in Firebreak Belt
Xu Ming
[Abstract](94) [FullText HTML](78) [PDF 585KB](3)
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Objective To screen and optimize the strain and apply it to the degradation of dead leaves of landscape tree species in forest firebreak belt for recycling of dead leaves and forest fire prevention. Method The highly degrading strains were screened out by the degradation methods of leaf strips of tree species in color bio-fireproof forest belt and the degradation weight loss methods. The highly degrading strains and their combinations were selected by the experimental analysis of the degradation weight loss effect of the highly degrading strains and their combinations. In addition, their degradation rules on the dead branches and leaves were analyzed. Result Through the degradation experiments of 11 strains on deciduous leaves and dead branches and leaves of 12 colorful landscape trees in the firebreak belt, Postia Placenta (Pp),Trichoderma Koningii (Tk1) and Polystictus versicolor (Pv) were found to have strong degradation ability and weight loss effect. The weight loss of Sassafras tsumu, Photinia × fraseri and Schima superba were 54.3% ± 2.3% and 62.1% ± 3.3% respectively. Conclusion Pp + Pv, Pv + Tk1 and Pp + Tk1 have significant degradation effect on the tree species in the forest firebreak belts, which can be widely used as in the forest firebreak belts in the south of Jiangsu Province.
Average Tree Height-Diameter Models of Cunninghamia lanceolata in China Based on Continuous Forest Inventory Plot Data
NIU Si-yuan, LIU Peng-ju, LEI Xiang-dong, REN Yi, GAO Ying
[Abstract](270) [FullText HTML](190) [PDF 879KB](9)
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Objective Based on the national permanent forest plots, the basic average tree height - diameter models of Cunninghamia lanceolate was established by province for predicting tree height . Methods There was a total of 23 239 samples distributed in 15 provinces in this study.. Eighteen candidate base growth equations were used to fit height-diameter relationship in each province. The coefficient of determination (R2), mean absolute error (MAE), mean relative error (MRE) and root mean square error (RMSE) combing with the residual plots were used for model evaluation. In addition, the 5-fold method was used to test the optimal model in each province. Result The best height-diameter model in each province was not the same. The best model for Chinese fir in Sichuan, Yunnan, Chongqing, Shanxi, Zhejiang, Jiangxi, Hunan and Guangxi was the Mitscherlich equation. For Jiangsu, Anhui, Henan and Fujian, the best model was the Hossfeld equation, And for Guangdong, Hubei and Guizhou, the best model was hyperbolic equation, Logistic equation and Gompertz equation, respectively. The R2 of the model ranged from 0.602 to 0.807, MAE ranged from 0.94 to 1.53 m, MRE ranged from −4.72 to −2.93%, RMSE ranged from 1.23 to 2.00 m, and MPE ranged from 0.50 to 2.77. These models performed well and had biological significance, which indicated that these models could be used as the basic height-diameter models of C. lanceolate plantation in each province Conclusion height-diameter models of C. lanceolate distributed in 15 provinces are developed in this study, which simulates well the average tree height of C. lanceolate in each province, and can be used as basic models of height-diameter for C. lanceolate plantation in each province in China.
Effects of Arbuscular Mycorrhizal Fungi Inoculation and Different Forms of Nitrogen Addition on Soil Nitrogen and Phosphorus Contents and Enzyme Stoichiometry in the Rhizosphere of Chinese Fir Seedlings
LEI Mei, GAN Zi-ying, TAN Shi-guang, SONG Xiu-ling, SHANG-GUAN Han-ting, QIU Qing-yan
[Abstract](180) [FullText HTML](76) [PDF 804KB](4)
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Objective To understand the influences of arbuscular mycorrhizal fungi (AMF) inoculation and different forms of nitrogen (N) addition on soil nitrogen (N) and phosphorus (P) nutrients, soil N and P enzyme activities and their enzyme stoichiometry in the rhizosphere of Chinese fir (Cunninghamia lanceolata) for providing theoretical basis of sustainable management of Chinese fir plantations. Method In the present study, a pot experiments were carried out to investigate the AMF inoculation (G. mosseae, Gm) and different forms of N (NH4 + -N, NO3-N) addition on soil N and P nutrients, related enzyme activities and their stoichiometry of one-year-old Chinese fir seedlings. Result The results showed that: (1) AMF inoculation increased soil available P content, but decreased the NO3-N, NH4 + -N, DON and total P content. Compared with NH4 + -N treatment, AMF under NO3-N treatment had more significant effect on soil N and P nutrients (P<0.05). (2) AMF inoculation and different forms of N addition increased the activities of soil acid phosphatase (AP), urease (URE), and N-acetyl-β-D glucosidase (NAG). Inoculation of AMF under NH4 + -N treatment was more conducive to improve urease activity, and inoculation of AMF under NO3-N treatment was more conducive to improve N-acetyl-β-D glucosidase activity; (3) AMF inoculation reduced the ratios of soil URE: AP, PRO: AP and NAG: AP (p<0.05), and the decrease of soil URE: AP, PRO: AP stoichiometry under NO3-N treatment was higher than that under NH4-N treatment.. Conclusion AMF inoculation can improve soil P availability, and increase the transfer of soil available N to the plants by increasing the activities of nitrogen and phosphorus-related enzyme in the soil and reducing the stoichiometry of nitrogen and phosphorus-related enzyme to maintain soil N and P balance. Moreover, the effects of NO3-N treatment on soil nitrogen and phosphorus balance is larger than that under NH4 + -N treatment.
Variation and Seasonal Difference of Corilagin Content in Leaves of Acer truncatum Bunge, Acer buergerianum Miq. and Acer paxii Franch.
SU Ya-jing, LU Yi-zeng, QIAO Qian, SUN Zhong-kui, WU Chong, YAN Yu, CHENG Tian-tian, ZHANG Lin, FENG Zhen
[Abstract](154) [FullText HTML](74) [PDF 800KB](2)
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Objective To determine the content of corilagin in leaves of Acer truncatum Bunge, A. buergerianum Miq. and A. paxii Franch. Methods The ultra-performance liquid chromatography (UPLC) were used. XBridge C18 (4.6 × 150 mm, 3.5 µm) was used as the chromatographic column, and 0.4% formic acid aqueous solution and methanol as the mobile phase with the flow rate of 0.3 mL·min−1, the column temperature of 25 ℃, the detection wavelength of 270 nm, and the injection volume of 2 μL. Results The leaves of A. truncatum Bunge, A. buergerianum Miq. and A. paxii Franch. contained corilagin. There were significant differences in the content of corilagin among different tree species. The content in A. buergerianum Miq. was the highest, followed by A. paxii Franch. and A. truncatum Bunge. There were also significant differences in the content of corilagin among different varities. Among the four varieties of A. truncatum Bunge, ‘Lvbaoshi’ reached the highest value of 0.607 9 mg·g−1, the average content in ‘Xingwang’ among the four cultivars of A. buergerianum Miq. was the highest, and ‘Qianlihong’ had the highest value (6.888 7 mg·g−1) in October, and the highest valuein A. paxii Franch. ‘Lulv’ was 2.664 0 mg·g−1. In addition, there was also a big difference in the content of corilagin at different harvest times. The highest values of A. truncatum Bunge and A. paxii Franch. mostly appeared in May and June, and the best harvesting time of A. buergerianum Miq. was in October. Conclusion This is the first time for revealing that there is corilagin in the leaves of A. truncatum Bunge, A. buergerianum Miq. and A. paxii Franch., among which A. buergerianum Miq. is more suitable as the raw material for extracting corilagin, and A. truncatum Bunge and A. paxii Franch. can be used as candidate resources.
Spatial Distribution Characteristics of Soil Soluble Nitrogen Component in Hippophae rhamnoides Plantation with Different Stand Ages in Loess Hilly Region
ZHAO Man-xing, MA Wen-quan, ZHANG Xia, MA Zhou, BAI Er-lei, NAN Guo-wei
[Abstract](120) [FullText HTML](94) [PDF 538KB](5)
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Objective To investigate the spatial distribution characteristics of soil soluble nitrogen component in Hippophae rhamnoides L. subsp. sinensis Rousi plantation with different stand ages in loess hilly region. Methods Based on the Hippophae rhamnoides plantation with three different stand ages of5-year-old, 15-year-old and 20-year-old were selected in Jinding Town of Zhidan County, soil samples were collected in 0-10 cm, 10-20 cm and 20-30 cm soil layers, and seasonal dynamic variation of the proportion of soil soluble nitrogen component were analyzed. The grassland was treated as control. Results With the extension of the recovery years, the content of soil soluble nitrogen components in the Hippophae rhamnoides plantations increased. For soil nitrate nitrogen and soluble organic nitrogen (SON), there were significant differences between Hippophae rhamnoides plantations in 20 years and 5 years, while there was no significant difference between grassland and Hippophae rhamnoides forests in 5 years. There was no significant difference in soil ammonium nitrogen among different restoration years. The soil nitrate nitrogen and SON showed the same trend, with 20-year-old>15-year-old> grassland>5-year-old. For the ammonium nitrogen, soluble organic nitrogen in forests with 15-year-old was the largest, followed by 20-year-old,5-year-old, and grassland, and the average content of ammonium nitrogen in the surface layer was the lowest. There was no significant difference in soil soluble nitrogen components among different soil layers. Soil nitrate nitrogen was the highest in summer and the lowest in winter or autumn. Ammonium nitrogen was the highest in summer or autumn, and the lowest in winter. SON was higher in spring and winter than in summer and autumn. The proportion of SON, nitrate nitrogen and ammonium nitrogen in total soluble nitrogen was 80.3%, 10.2% and 9.5% respectively. The proportion of SON in total soluble nitrogen of Hippophae rhamnoides forests with different years was higher in spring and winter than in summer and autumn, and most of nitrate nitrogen and ammonium nitrogen were higher in summer and autumn than in spring and winter. Conclusion In the loess hilly area of northern Shaanxi, Hippophae rhamnoides plantations can effectively improve soil soluble nitrogen components. With the increase of forest age, the soil nitrogen accumulation increases.
Genetic Analysis of the Fruit and Oil Related Traits on Hybrid Offspring of Nested Mating of Camellia oleifera
CHAI Jing-yu, WANG Kai-liang, YAO Xiao-hua, TENG Jian-hua, LIN Ping
[Abstract](106) [FullText HTML](57) [PDF 605KB](3)
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Objective To Select the optimal parent and parental combination for improving breeding efficiency and maximize genetic gain in oil-tea hybrid breeding. Method In this study, 11 fruit and oil related traits were detected in the intraspecific and interspecific hybrid progeny in nest design, including weight of single fruit, seed rate of fresh fruit, kernel rate of dry seeds, oil content of kernel and seven kinds of fatty acid content in the oil. The general combining ability (GCA) of female parent and special combining ability (SCA) of hybridized combination were analyzed. The genetic control modes for 11 traits were analyzed. Result There were highly significant differences in 11 traits among 12 families. These differences mainly caused by paternal effects at the level of P < 0.01, and only the differences of stearic acid and linoleic acid content were affected by maternal effects at the level of P < 0.05. The GCA and SCA of the parents were comprehensively analyzed. The intraspecific hybridization ‘Changlin No.4’ × ‘Changlin No.23’ showed better combining ability in weight of single fruit, seed rate of fresh fruit, kernel rate of dry seeds, oil content of kernel and oleic acid content in oil. The interspecific hybridization of Camellia oleifera and C. meiocarpa ‘Changlin No. 53’ × ‘Xiao No.3’ had a better performance on weight of single fruit, palmitic acid, linoleic acid and linolenic acid content. The cross of ‘Changlin No. 53’ × ‘Xiao No.2’ had advantages in seed rate of fresh fruit, kernel rate of dry seeds, oil content of kernel and oleic acid content. The analysis of genetic effect showed that stearic acid, oleic acid and linoleic acid content were mainly controlled by additive genetic effect, while the other eight traits were mainly influenced by non-additive genetic effect. The heritability of 11 economic traits of per plant was greater than that of families. The breeding strategy of selecting excellent individual plants within the full-sib families should be adopted in the cross breeding of C. oleifera. Conclusion The economic traits of C. oleifera are mainly controlled by inheritance, and less affected by environment. Weight of single fruit, seed rate of fresh fruit, kernel rate of dry seeds, oil content of kernel, palmitic acid, palmitoleic acid, linolenic acid and cis-11-eicosenoic acid content were mainly controlled by non-additive gene effect, and the influence of additive gene effect is relatively low. In interspecific hybridization, SCA evaluation of hybridized combination should be given priority. In this study, we explored the parental allocation and genetic effects of interspecific hybridization between C. oleifera and C. meiocarpa, which laid a theoretical foundation for guiding interspecific hybridization breeding of C.oleifera.
Response of Chestnut Quality of 'Yanshanzaofeng' to Climate Factors in Different Cultivation Areas
FAN Xiao-yun, GUO Su-juan, JIANG Xi-bing, LI Yan-hua
[Abstract](112) [FullText HTML](55) [PDF 592KB](1)
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Objective To explore the key climate factors affecting the quality of 'Yanshanzaofeng' chestnut in different cultivation areas and establish the key climate factors evaluation system of 'Yanshanzaofeng' cultivation for providing theoretical basis for the scientific planting and introduction of 'Yanshanzaofeng'. Methods Based on 'Yanshanzaofeng' planted in eight different growing areas, the correlation and regression analysis were carried out between nut quality indexes and climate factors, and the nonlinear regression equation between nut quality indexes and key climate factors was constructed. Results The results showed that the cross diameter (33.63 mm), longitudinal diameter (26.98 mm), fruit shape index (0.73) and single grain weight (10.86 g) of 'Yanshanzaofeng' in Jinhua cultivation area were higher than those in other cultivation areas. The soluble sugar content (19.01%) and amylopectin/amylose ratio (1.69) in qianxi cultivation area of Hebei province were higher than those in other areas. The key climate factors affecting the quality of 'Yanshanzaofeng' nuts were temperature, light and water, and the daily range of average temperature in growing season had the greatest influence on the quality of nuts. The nut quality of 'Yanshanzaofeng' was the best in the ecological area with a daily average temperature difference of 25.89℃ in the growing season. The optimal value of quality indexes were soluble sugar 25.63%, amylopectin/amylose ratio 1.93, protein 8.32%. Conclusion Climatic conditions such as longer sunshine duration, higher average temperature and greater diurnal range of average temperature in growing season are conducive to the formation of sweet and waxy quality of 'Yanshanzaofeng'.
Analysis of Complete Chloroplast Genome Sequences and Phylogenetic Evolution of Zanthoxylum armatum‘Jiuyeqing’
LIU Xia, SUN Chong, HUANG Qin-qin, LIU Hao-wen, Huang Feng-ting, CHEN Ze-xiong
[Abstract](303) [FullText HTML](196) [PDF 1073KB](8)
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Objective To reveal the structural characteristics and phylogenetic relationships of the chloroplast genomes of Zanthoxylum armatum ‘Jiuyeqing’, and provide reference for identifying germplasm resources, breeding new varieties and the genetic analysis among varieties of the genus Zanthoxylum. Method The total DNA of Z. armatum ‘Jiuyeqing’ was extracted using a modified CTAB method. High-throughput sequencing was performed using the BGISeq-500 platform, the chloroplast genome was assembled by SPAdes v3.13.0 software, the whole chloroplast genome information of Z. armatum ‘Jiuyeqing’ was annotated by GeSeq software, and the structural characteristics, repetitive sequences, codon preference and phylogenetic relationship were analyzed. Result Z. armatum ‘Jiuyeqing’ was a typical tetrad structure with a full-length sequence of 158 558 bp, encoding 133 genes. 19 tandem repeats were detected, 49 long repeat sequences, 70 simple sequence repeat (SSR) were detected. A total of 26 398 codons (excluding stop codons) were detected in the protein-coding gene of the chloroplast genome of Z. armatum ‘Jiuyeqing’, and there was a strong A/T base preference at the third base of the codon. Conclusion The complete chloroplast genome sequences of Z. armatum 'Jiuyeqing' was firstly assembled for the study. The results of phylogenetic analysis show that the genus Zanthoxylum is a monophyletic group, and the Z. armatum 'Jiuyeqing' is closely related to Z. simulans. The results of the study enrich the genetic information of Zanthoxylum and improve important references for Zanthoxylum germplasm resource evaluation, molecular breeding, development of cpSSR molecular markers, and genetic diversity research.
Genome-wide Identification and Expression Analysis of BpAMT Gene Family in Betula platyphylla
Yang Hai-xin, Liu Xiao-ying, Zhan Ya-guang, Fan Gui-zhi
[Abstract](564) [FullText HTML](355) [PDF 2093KB](20)
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Objective To identify AMT gene family members in Betula platyphylla and analyze the expression pattern of AMT genes. Method Bioinformatics methods were used to identify the family genes and real-time fluorescence quantitative analysis of gene expression. Result 9 AMT genes were identified from the B.platyphylla genome and divided into two subfamilies, AMT1 and AMT2, named BpAMT1.1-1.4 and BpAMT2.1-2.5; These BpAMT proteins contained 384-522 amino acid residues with isoelectric points ranging from 4.61 to 8.16, and all of which were located on the plasma membrane and organelle membrane; The genes of BpAMT family were distributed on 5 chromosomes unevenly, and there were tandem duplication among the members. BpAMTs’ expression patterns had specificity in different tissues, showing a leaf>root>stem trend; At the same time, KNO3, NH4Cl, MeJA, GA3, ABA, CdCl2 and diurnal variation could affect the expression of BpAMT gene, and the response of members differed under different treatments. Conclusion 9 BpAMT genes are identified and divided into two subfamilies. BpAMT gene plays an important role in regulating nitrogen uptake, transport and in response to hormone signals or abiotic stress. These results lay a foundation for further analysis of the function of BpAMT gene in growth and resistance to stress in B.platyphylla.
Age-independent Dominant Height Growth Model for Pinus massoniana Plantation
ZHANG Ke-xin, LIU Xian-zhao, GUO Hong, LIU Dan, LEI Yuan-cai
[Abstract](515) [FullText HTML](307) [PDF 745KB](11)
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Objective An age-independent theoretical growth equation was constructed to predict the dominant height of Pinus massoniana plantation when the age was unknown or not an effective influence factor. Methods Based on the four theoretical growth equations of Hossfeld IV, Lundqvist-Kolf, Richards and Schumacher, an age-independent dominant height model of Pinus massoniana was constructed by using the difference form of hidden age factors, and the free parameters were expressed as a function of site index. Results Among the four basic theoretical growth models, the age-related dominant growth model of Pinus massoniana plantation established by Richards model performed the best. The fitting accuracy of age-independent models was higher than that of age-related models, and the age-independent dominant growth model of Pinus massoniana plantation established by Richards model and Korf model performed the best. When the site index was introduced to the model, the fitting accuracy of Hossfeld model improved most obviously, and when K was set as a variable parameter, the age-independent dominant high growth model was the best. Conclusion When the forest of different age or age is difficult to obtain, the theoretical growth model independent of age can predict the growth of dominant height. After the functional relationship between site index and free parameters is replaced in the model, the accuracy of the model is improved and the model can be better applied to predict stand growth.
Identification of MicroRNAs during Seed Germination and Its Response to PEG and NaCl Stresses
Wang Xiao-jing, Wang Tao, Yang Kai, Li Lu-bin
[Abstract](810) [FullText HTML](377) [PDF 1112KB](9)
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Objective To identify microRNAs (miRNAs) and reveal its expressional pattern in seed coat rupture stage of Moso bamboo seeds (Phyllostachys edulis) under different drought and salt stresses. Methods Polyethylene glycol (PEG6000) and NaCl were used to simulate drought and salinity stress, respectively. Small RNA libraries were separately built for Moso bamboo seeds germinated under H2O, 10% PEG, 15% PEG, 50 mmol·L−1 NaCl and 100 mmol·L−1 NaCl, and the seeds were all sampled at seed coat rupture stage. High throughput sequencing and bioinformatics analysis were used to explore the expressional pattern of miRNA. Results A total of 246 known miRNAs and 262 novel mature miRNAs were identified in this study. The most abundant miRNAs in seed coat rupture stage of Moso bamboo was miR166, followed by miR159, miR6478, miR319, etc. According to miRNA target prediction, MIR396 family owned the largest number of target genes, and ph02gene13935 (GAMYB) could to be regulated by 28 miRNAs of MIR159, MIR319 or MIR396; A total of 181 differentially expressed miRNAs (DEmiRNA) were identified in six comparison groups; Compared with control group, in 10% PEG, 15% PEG, 50 mmol·L−1 NaCl and 100 mmol·L−1 NaCl treatments, phe-miR171e-5p, phe-miR3630-3p, phe-miR171e-5p and phe-miR159a were differentially expressed respectively with highest expressional level in known miRNAs; The target genes of DEmiRNA were significantly enriched in different GO and KEGG pathways; Ten DEmiRNAs were verified by qPCR, and the overall trend of qPCR results was consistent with the sequencing data. Conclusion In seed coat rupture stage of moso bamboo, there exhibit high accumulation of known miRNAs such as miR159, miR6478, miR319 in all control and four treatment groups, which may play a conservative regulatory role in Moso bamboo seed germination. Compared with the control group, phe-miR171e-5p, phe-miR3630-3p, phe-miR171e-5p and phe-miR159a 1 are differentially expressed in 10% PEG, 15% PEG, 50 mmol·L−1 NaCl and 100 mmol·L−1 NaCl, respectively, which can respond to PEG or NaCl stress during seed coat rupture stage of Moso bamboo.
Effects of PagWOX11/12a Gene on Stem Growth and Development of Populus alba × P. glandulosa
Wen Shuang-Shuang, Wang Liu-Qiang, Lu Meng-Zhu
[Abstract](899) [FullText HTML](446) [PDF 2651KB](16)
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Objective To analyze the effect of PagWOX11/12a gene on the growth and development of poplar for further research on the regulation mechanism of this gene in woody plants. Method Bioinformatics methods and software were used to construct phylogenetic evolutionary tree, sequence alignment and biochemical characterization analysis. Tissue-specific expression patterns were analyzed by qRT-PCR. The phenotype of poplar after specifically suppressed the expression of PagWOX11/12a was analyzed by using transgenic plant 35S::PagWOX11/12a-SRDX (DR). Result PagWOX11/12a gene could encode a protein with 255 amino acids, which was expressed in different tissues of 84K. The phenotypic analysis of DR transgenic plants showed that inhibiting the expression of this gene could reduce the length of phloem cells, pith cells and xylem fiber cells, inhibit internode elongation, and significantly reduced plant height compared with non-transgenic 84K. Conclusion PagWOX11/12a gene participates in regulating the height growth of poplar by affecting the elongation of internodes. This study provides a reference for further revealing the regulatory mechanism of PagWOX11/12a gene involved in the growth and development of poplar.
Community composition and ecological functional analysis of the endophytic microorganisms in Eucommia ulmoides seeds
ZHANG Qing-Qing, DONG Chun-Bo, SHAO Qiu-Yu, LU Ying-Xia, DONG Xuan, LIANG Zong-Qi, HAN Yan-Feng
[Abstract](1350) [FullText HTML](657) [PDF 1126KB](49)
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Objective To reveal the microbial community composition and diversity in Eucommia ulmoides seeds and enrich the related microbial resources. Methods In this study, the diversity of endophytic microorganisms in E. ulmoides seeds was analyzed by traditional culture method and high-throughput sequencing technology, and functional annotated by FAPROTAX and FUNGuild database. Results A total of 40 fungi strains (3 phyla and 11 genera) were isolated from E. ulmoides seeds, and the dominant genus was Aspergillus (30%); 142 bacterial strains (1 phylum, 6 genera) were isolated, of which Solibacillus was the dominant genus (47.18%). Through high-throughput sequencing, 141 fungal OTUs were obtained belonging to 6 phylum and 101 genera; 442 bacterial OTUs were obtained belonging to 24 phylum and 313 genera. At the genus level, the dominant fungi were Apiotrichum (31.28%) and Debaryomyces (26.07%); The dominant bacterial genera were Pseudomonas (16.66%) and Lactobacillus (9.68%). The results of functional annotation showed that fungi mainly degraded organic matter, promoted the growth of host plants and the absorption of soil nutrients by host, improved soil microbial community structure and Pathogen community. The bacteria mainly consisted of chemoheterotrophic, heterotrophic, fermentation. and nitrogen fixation bacteria. Conclusion E. ulmoides seeds carry a variety of probiotics and potential phytopathogens, which have important effects on plant growth and development. In addition, a number of beneficial functional bacteria were isolated in this study, which will provide microbial resources for the subsequent artificial control of seed microbiome to enhance plant health.
Effects of light and N:P ratio on photosynthetic characteristics in seedlings of three typical tree species
XIANG Wang, CHENG Xiang-Rong, XU Hai-Dong, LIU Jiang-Wei
[Abstract](939) [FullText HTML](606) [PDF 824KB](20)
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Objective To study the response of photosynthetic characteristics of different ecological tree species to light and nutrient supply for providing a scientific basis for plantation cultivation. Method The seedlings of Quercus acutissima, Phoebe bournei, and Schima superba were used. This study included two light intensities (natural full light and 45% full light) and three N:P ratios (N:P=5, N:P=15, N:P=45). We measured the changes of Photosynthetic fluorescence parameters, and seedlings’ height, seedlings’ ground diameter to study the changes of plant photosynthetic characteristics in different environments, and analyze the relationship between seedling growth and photosynthetic parameters. Result (1) Compared with the full light treatment, the shading treatment decreased the light saturation point (LSP) and the maximum net photosynthetic rate (Pnmax) of Q. acutissima, and increased the Pnmax of S. superba. but had no significant effect on Pnmax of P. bournei. Under the two light treatments, ertilization increased the LSP and light compensation point (LCP) of P. bournei and S. superba, but the effects of fertilization on the shading LSP and LCP of Q. acutissima were less. (2) Shading significantly reduced the quantum yield of regulated energy dissipation of PSⅡ [Y(NPQ) ] of Q. acutissima, P. bournei and S. superba, and significantly increased the photosynthetic electron transport rate (ETR). Under the full light treatments, fertilization generally increased the effective quantum yield of PSⅡ [Y(II) ] and ETR of Q. acutissima, P. bournei and S. superba, but decreased Y(NPQ) . Under the shading treatments, fertilization significantly increased Y(II) and ETR of P. bournei, while Y(II) and ETR of Q. acutissima increased significantly when the N:P supply ratio was 5. Y(II) and ETR of S. superba increased significantly when the N:P supply ratio was 15. (3) Compared with the full light treatment, shading significantly increased the contents of chlorophyll a, chlorophyll b, chlorophyll a + b and carotenoid of Q. acutissima, P. bournei and S. superba. Under the two light treatments, fertilization significantly increased the photosynthetic pigment content of Q. acutissima, S. superba and P. bournei (only full light treatment). (4) Regardless of light or shading, fertilization generally increased the relative growth rates of seedling’s height and ground diameter of the three tree species, and there was a positive correlation between the growth and photosynthetic pigment. Conclusion Photosynthetic characteristics and growth of Q. acutissima, P. bournei and S. superba are affected by the interaction of light and fertilization. The effect of nitrogen and phosphorus fertilization on seedling growth under different light intensities depends on nitrogen and phosphorus supply ratio and tree species.
Transcriptome Analysis of Sonneratia apetala Root in Response to Salt Stress
LIANG Rui-tao, HAN Wei-dong, YANG Shao-xia, CHEN Bei-bei
[Abstract](780) [FullText HTML](391) [PDF 1529KB](9)
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Objective To lay a molecular basis for functional verification experiment and genetic breeding of tree salinity tolerance, the genetic mechanisms was explored, and salt-related genes were identified for Sonneratia apetala Buch.-Ham. Method In this study, the root tissues were collected from a 1-year-old S. apetala treated with 500 mmol•L−1 NaCl for 0 (control group) and 10 d (treatment group). Then transcriptome sequencing and bioinformatics analysis was performed based on the three-generation full-length transcriptome dataset of S. apetala. Result (1) Compared with the control group, 14401 genes were differentially expressed after salt treatment, of which 7153 were up-regulated and 7248 were down-regulated. (2) GO analysis found that a total of 11068 differential genes were annotated in 47 GO items. (3) For KEGG enrichment analysis, a total of 6189 differential expression genes were enriched to 134 pathways, of which 14 were significantly enriched (P-value <0.01, Q-value <0.05). (4) Further functional annotation analysis of the differentially expressed genes revealed a total of 89 genes was potential salt-related candidate genes. Among these, 24, 22, 19, 10 and 14 genes encoded enzymes or functional proteins referred to antioxidation, osmotic adjustment substances, plant hormones, protein kinase and transcription factors, respectively. Conclusion Genes relating to active oxygen scavenging, osmotic regulation, plant hormones, protein kinases and transcription factors participate in the regulation of salt stress adaptation in S. apetala.
Response of Radial Growth of Larix griffithii to Temperature and Precipitation Fluctuation in Tibet Shergyla Mountain
YU De-shui, LU Jie, ZHANG Meng, ZHANG Xin-sheng
[Abstract](854) [FullText HTML](476) [PDF 1307KB](14)
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Objective To study the response of radial growth of Larix griffithii Hoof to temperature and precipitation based on the annual ring core of L. griffithii collected in the Shergyla Mountain. Method The standard chronology was established by using the tree chronology method combined with TASP-Win and ARSTAN programs. Pearson and bootstrap calculation methods were used to analyze the correlation between ring width index and temperature and precipitation from 1961 to 2020. Result There were significant differences in radial growth before and after temperature fluctuation. Before the temperature fluctuation, the radial growth was positively correlated with the average temperature in January and June of the year and August of the previous year (P< 0.05). It was positively correlated with the average precipitation from September to October and December of the previous year and negatively correlated with that in August of the previous year. And It was significantly positively correlated with the relative humidity from January to December (P< 0.01). After the temperature fluctuation, the radial growth was negatively correlated with the average temperature from March to December and positively correlated with the precipitation in August and June of the previous year. Conclusion The climatic conditions in Southeast Tibet are extremely special. The uneven hydrothermal conditions caused by climate fluctuations is the main reason for the radial growth change of L griffithii. AThe short-term change of temperature in the non-growing season also has a significant indigenous impact on the radial growth of trees.
Investigation of Arbuscular mycorrhizal fungi (AMF) in several alpine oak understory forests in the subalpine zone of the Hengduan mountains
Li Hong-bo, Huang Yao-hua, Kang Ding-xu, Wu Jian-rong, Ma Huan-cheng, Chen Shi
[Abstract](1284) [FullText HTML](828) [PDF 2916KB](9)
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Objective To clarify the taxonomic status of AMF spores under the oak forest in the subalpine belt of the Hengduan Mountains. Method Six samples of alpine oak root enclosures and root systems were selected from different parts of the subalpine belt of the Hengduan Mountains, and AMF spores in the soil were selected by wet sieving sedimentation method. AMF-specific primers were used to amplify the 18SrRNA gene of the ITS region, which was identified by combining morphological and molecular biological means, and the phylogenetic relationships were constructed to infer their affinities. Result The results showed that AMF spores and root cloning consequences of alpine oak in Sichuan and Tibet were the genus Paraglomus. And the consequence of AMF cloning on the root surface and the AMF in soil of alpine oak in Shangri-La showed that AMF could exist in the inter-roots of alpine oak plants. These AMF were the genus Glomus. In addition, only one AMF was found in the root envelope and inter-root soil of alpine oak forest in different sampling sites, suggesting that AMF might have a symbiotic relationship with alpine oak. Aniline blue pigment staining result showed obvious arbuscular and vesicle structures in roots. In addition, the AMF spores under the Alpine oak forest differentiated during the evolution and were between the taxonomic status of Paraglomus and Glomus, and there were differences in AMF in Tibet, Sichuan and Shangri-La, Yunnan. Conclusion AMF generally exists in the pure understory root rhizosphere and inter-root soil of alpine oak in the subalpine belt of the Hengduan Mountains, which provides a theoretical reference for understanding the formation and evolution of AMF in the understory of alpine oak in the subalpine belt of the Hengduan Mountains
Physiological and transcriptomic analysis of Catalpa bungei seedlings in response to saline-alkali stresses
Gao Xin-qiang, Wang Xiao-yan, Jiao Wei, Wang Jing, Zheng Li-yue, Wang Dan-ning, Wang Xing-yun, Hou Li-jiang, Shang Zeng-zhen, Liu Yan-zhen
[Abstract](1888) [FullText HTML](626) [PDF 2385KB](15)
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Objective To explore the physiological and molecular mechanism of saline-alkali tolerance in Catalpa bungei, we studied the influence of different saline-alkali stress on the growth, photosynthetic and physiological indicators of C. bungei seedlings combing with transcriptome sequencing. Methods Pot experiment was used to study the different responses of biomass, photosynthetic and physiological indicators of C. bungei seedlings to different saline-alkali stress. Illumina high-throughput sequence technology was used to sequence the transcriptome, and the effect of saline-alkali stress on transcriptional level was analyzed by bioinformatics. Results Under different saline-alkali stress, the damage degree of leaves was Na2CO3>mixed saline-alkali>NaCl. Net growth of plant height and stem diameter, fresh weight and dry weight of overground part and root, biomass, root-shoot ratio were all significantly suppressed with increasing saline-alkali concentration. But the growth stress index decreased with increasing concentration. Contents of MDA and relative conductivity both rose to varying degrees with increasing concentration. SOD enzymatic activity, contents of soluble sugar and Pro, contents of total chlorophyll and photosynthetic rate increased firstly and decreased then with increasing concentration. Transcriptome sequencing generated a total of 60.4Gb of raw data. Finally, we obtained 55 793 Unigenes after assembling, of which 29 534 (52.93%) Unigeneswere annotated. Through differentially expressed genes (DEGs) analysis, 1 779, 2 835 and 4 059 DEGs were screened from three comparison groups (CK vs NaCl, CK vs Na2CO3 and CK vs mixed saline-alkali) respectively. GO functional enrichment analysis of these DEGs indicated that they were significantly enriched in integral component of membrane, intrinsic component of membrane, catalytic activity, isoprenoid metabolic and biosynthetic process, oxidoreductase activity. KEGG functional enrichment analysis of these DEGs indicated that they were significantly enriched in phenylpropanoid biosynthesis, starch and sucrose metabolism, plant hormone signal transduction, terpenoid backbone biosynthesis and arginine biosynthesis. Moreover, the most abundant differentially expressed transcription factors (TFs) were bHLH, ERF, MYB-related, NAC, C2H2, WRKY, MYB and bZIP families. Conclusion C. bungei mainly resists from saline-alkali stress by accumulating contents of soluble sugars and Pro, improving SOD enzymatic activity and photosynthesis, but all of them show the phenomenon of "low promotion and high suppression", indicating that it has a certain threshold value. C. bungei common responses to saline-alkali stress by regulating biological processes and metabolic pathways including component of membrane, catalytic activity, isoprenoid metabolic and biosynthetic process, phenylpropanoid biosynthesis, starch and sucrose metabolism, plant hormone signal transduction, and interacting with TFs. This study provides a scientific theoretical basis for deeply studying the physiological and molecular mechanisms of saline-alkali tolerance in C. bungei.
Investigation and Analysis on Host Species and Their Resistance Substances to Bacterial Bark Canker of Populus × euramericana
LI Yong, CHANG Ju-pu, GUO Li-min, SUN Shang, YANG Xu-qi, PIAO Chun-gen
[Abstract](3432) [FullText HTML](2084) [PDF 1005KB](12)
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Objective To investigate and analyze the host species of bacterial canker of Populus × euramericana, the disease-resistant varieties and the possible disease-resistant substances they contain. Method The host of the pathogen and the resistant varieties were determined by field investigation and inoculation tests. The content of active substances in the bark of disease-resistant and susceptible varieties was analyzed by HPLC. Result The hosts of bacterial pathogen of P. × euramericana canker are P. deltoides, P. × euramericana and Salix matsudana. P. × euramericana cl. 'Zhonglin 46', P. deltoides cl. '2025', P. × euramericana cv. '2001', P. deltoides cl '313', P. deltoides 'Zhonghe-1' and P. deltoides '16-09' were highly susceptible to the bark canker disease; P. × euramericana 'Yukang 1' and P. deltoides '16-10' were highly resistant varieties, P. deltoides '131', P. deltoides '03-59', P. deltoides '02-212' and P. deltoides '03-34' were resistant varieties. Hybrid willows and Salix babylonica are resistant to the pathogen, and Salix × aureo-pendula can be affected, but do not cause serious disease. The HPLC analysis showed that the contents of catechol, benzoic acid and chlorogenic acid in the bark of resistant and highly susceptible poplar varieties were significantly different. The catechol and catechin increased when the hosts were infected by Lonsdalea populi. Conclusion In this study, the host species of bacterial canker of P. × euramericana are determined, and resistant and highly susceptible poplar varieties are screened. It is showed the catechol and catechin are important compound for host to defense the pathogen infection. The results will be helpful to the control of the bacterial disease of poplar.
Effects of Castanopsis kawakamii Forest Litter on Seed Germination of Pinus massoniana
JIA Mei-hua, WANG Zhe, LIU Jin-fu, JIN Meng-ran, HE Zhong-sheng, XING Cong, SHI You-wen, SHEN Cai-xia
[Abstract](4264) [FullText HTML](2696) [PDF 935KB](38)
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Objective To understand the effect of litter of Castanopsis kawakamii natural forest on the competitive intensity of regeneration of Pinus massoniana. Method The allelopathic effects of litter extract of natural forest with different concentration and decomposition degree on seed germination and radicle growth of P. massoniana were studied. Result (1) The main factor affecting the germination of P. massoniana seeds was the degree of litter decomposition. Compared with the blank control, the litter of undecomposed layer and fully decomposed layer had no significant effect on the germination of P. massoniana seeds; the semi-decomposed layer inhibited the germination rate and germination index and promoted the germination potential. (2) The main factors affecting the growth of radicle were the concentration and action time of litter extract, and the effect of undecomposed layer on the whole radicle growth period of P. massoniana seeds was enhanced with the decrease of concentration, but increased with the decrease of concentration, and the promoting effect was the strongest at the concentration of 1 ≤ 100, and the effect of semi-decomposition layer was promoted by low concentration and high concentration, and the intermediate concentration was inhibited by the undecomposed layer. The fully decomposition layer showed promoting effect as a whole, and the promoting effect decreased with the decrease of concentration. (3) Correspondence analysis showed that different decomposition degree-concentration had no significant effect on seed germination of P. massoniana, but had significant correlation with radicle growth. Conclusion The result proves that the litter extract of different decomposition degree has a weak promoting or inhibiting effect on the seed germination of P. massoniana, promotes the growth of radicle at low concentration, and promotes the regeneration of P. massoniana, which will intensify the interspecific competition in the forest, and lead to the inhibition of the natural regeneration of C. kawakamii.
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2022, 35(6).  
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2022, 35(6): 0-0.  
[Abstract](61) [FullText HTML](69) [PDF 272KB](8)
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The Response of Leaf Anatomical Structure and Photosynthetic Physiology of Pinus massoniana Lamb. and Castanopsis hystrix Miq. to Throughfall Reduction
GUO Xin-wei, LIU Shi-rong, WANG Hui, CHEN Zhi-cheng, NIE Xiu-qing, ZHANG Jing-lei, MING An-gang, CHEN Lin
2022, 35(6): 1-11.   doi: 10.13275/j.cnki.lykxyj.2022.006.001
[Abstract](1341) [FullText HTML](816) [PDF 1733KB](22)
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Objective To explore the response of leaf anatomy and leaf photosynthetic physiology to long-term drought and analyze the relationship between leaf anatomy and leaf photosynthetic physiology under long-term drought conditions. Method We conducted a manipulation drought experiment by reducing the throughfall in the forest and measured the leaf gas exchange, chlorophyll fluorescence, leaf nutrient (N, P) and leaf anatomy of the two typical tree species (Pinus massoniana Lamb. and Castanopsis hystrix Miq.) in subtropical China at the 8th year after the throughfall reduction treatment. Results (1) Significant differences were observed in leaf anatomy between P. massoniana and C. hystrix in response to throughfall reduction; the upper and lower epidermis, leaf thickness and stomatal density of P. massoniana needles increased significantly. However, the thickness of the upper epidermis of the leaves of C. hystrix increased significantly, and the thickness of the spongy tissue decreased significantly. (2) No significant differences were observed in the gas exchange parameters (net photosynthetic rate Pn, stomatal conductance Gs, transpiration rate Tr), chlorophyll fluorescence parameters (photosystem II maximum photochemical efficiency Fv/Fm, actual photochemical efficiency ΦPSII, apparent electron transfer rate ETR) and leaf nutrient contents of both species after 8 years of throughfall reduction. (3) Compared with P. massoniana, the stomatal limitation (Ls) of the C. hystrix was larger and the water use efficiency (WUE) was higher, especially in the dry season. Conclusion There are differences in the strategies of P. massoniana and C. hystrix to cope with drought stress. P. massoniana responds to drought by adjusting leaf anatomy to produce xeromorphic needles, while C. hystrix is more inclined to adjust stomata (Ls) and improve water use efficiency (WUE) to adapt to drought.
Comprehensive Evaluation of Nutrients and Antioxidant Components in Nuts of Chestnut
WU Yan-yan, SHI Wen-shi, SHI Xin-ru, CHEN Rong-rong, ZHAO Yue, ZHAO Jia-bing, JIANG Ze-ping, WANG Zhi-wei, SHI Sheng-qing
2022, 35(6): 12-22.   doi: 10.13275/j.cnki.lykxyj.2022.006.002
[Abstract](540) [FullText HTML](452) [PDF 650KB](17)
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Objective To explore the feasibility of antioxidant enzymes as germplasm evaluation indices of chestnut (Castanea mollissima) and improve the evaluation system of excellent germplasm resources. Method Eighteen chestnut germplasms were collected in the areas of Yanshan Mountains in Hebei Province. The appearance traits, nutrients and antioxidant components (including 4 enzymatic indexes: superoxide dismutase (SOD), polyphenol oxidase (PPO), peroxidase (POD), catalase (CAT), and 3 non-enzymatic indexes: total polyphenols, total flavonoids and vitamin C) were determined and the qualities of chestnut germplasms were evaluated through subordinate function and factor analysis (with or without antioxidant enzymes) to screen excellent germplasm resources. Result Among these germplasms, the average weight of single fruit was 9.65 g, the largest was TPZ (12.51 g), and the smallest was QTC (7.88 g); the average water content was 48.85%, the highest was TPZ (51.59%), and the lowest was LJT (45.95%); the average value of starch content was 46.81%, LJT was the highest (53.69%), and JCY-1 was the lowest (38.28%); the average value of soluble sugar content was 7.96%, ZQ1 was the highest (11.62%), and YHZ was the lowest (4.51%); the average value of SOD activity was 1 012.37 U·g−1, the highest was TPZ (1 310.42 U·g−1), and the lowest was ZQ1 (717.40 U·g−1); the average value of POD activity was 4 168.67 U·g−1, JIH was the highest (5 965.33 U·g−1), and JCY-3 was the lowest (3 009.33 U·g−1). Conclusion Regardless of whether four antioxidant enzymes were used or not in the comprehensive evaluation ranking, the top one was QX107, followed by QL3113, QX3113, and HHZ20. In addition to the above three cultivars, ZJ2 and ZQ1 could be used as candidate elite germplasms for further test. The antioxidant enzymes SOD, POD, CAT and PPO could be considered as the evaluation indices of chestnut germplasms in future.
Soil Aggregate Structure of Subalpine Woodland and Its Influence on Priority Path in Central Yunnan
LU Hua-xing, ZHAO Yang-yi, DUAN Xu, ZHU Meng-xue, TU Xiao-yun, YANG Wen-yan
2022, 35(6): 23-34.   doi: 10.13275/j.cnki.lykxyj.2022.006.003
[Abstract](660) [FullText HTML](442) [PDF 2441KB](21)
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Objective To analyze the influence of soil aggregate structure on the formation of soil priority path for subalpine woodland in central Yunnan for providing scientific basis for the construction of water conservation forest, the restoration and reconstruction of natural forest vegetation in central Yunnan forest area. Method Based on four subalpine woodland soils of Pinus yunnanensis forest, Pinus armandii forest, Quercus semecarpifolia forest and Keteleeria evelyniana forest in central Yunnan, the soil priority path was extracted by using dye tracing method, combined with Photoshop CS 5, image pro plus 6.0 and surfer 15 image processing software. The structural characteristics of soil aggregates were systematically evaluated, and the effect of soil aggregate structure on priority path was explored by correlation analysis, principal component analysis, grey correlation analysis and coupling degree analysis. Result Results showed that: (1) the soil of four subalpine forest lands in the study area had good aggregate structure, and the structural stability in the P. armandii forest was the largest, followed by K. evelyniana forest, P. yunnanensis forest, and Q. semecarpifolia forest. The soil aggregate structure was significantly affected by forest land type and soil depth (P < 0.05), and it was correlated with the content of water stable aggregates in different particle sizes. (2) The preferential flow of forest land soil had advantages. The connectivity of priority paths in P. armandii forest was better than that of P. yunnanensis forest, Q. semecarpifolia forest, and K. evelyniana forest. In addition, the number of priority paths gradually decreased with the increase of soil depth. (3) The content of water stable aggregates with particle size > 0.25 mm (WSA), mean weight diameter (MWD), geometric mean diameter (GMD), mean weight soil specific area (MWSSA), percentage of aggregate destruction (PAD) and fractal dimension (D) could better evaluate the structural characteristics of soil aggregates. The correlation with the number of priority paths was more than 0.62, which was important factor affecting the formation of priority paths. (4) The average coupling degree between soil aggregate structure and the number of priority paths for subalpine forest land in central Asia was 0.683, and the system belonged to weak coordination. The coupling coordination degree in P. yunnanensis forest was the largest (0.728), followed by P. armandii forest (0.681), Q. semecarpifolia forest (0.663), and K. evelyniana forest (0.662). Conclusion Soil aggregate structure is an important factor that affects the formation and occurrence of soil priority path, but it does not play a decisive role in the formation of priority path.
Effects of Litter and Nitrogen Addition on Carbon and Nitrogen in Soil Leaching Solution of Subtropical Castanopsis fabric and Cunninghamia lanceolata Forest
WANG Meng-si, MA Hong-liang, GUAN Xiao-hui, GAO Ren, YIN Yun-feng
2022, 35(6): 35-43.   doi: 10.13275/j.cnki.lykxyj.2022.006.004
[Abstract](1397) [FullText HTML](732) [PDF 810KB](18)
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Objective Litter is the main source of carbon and nitrogen in forest soils. The effect of litter decomposition on soil carbon and nitrogen by leaching was studied through analyzing the changes of dissolved carbon and nitrogen in litter or soil leaching solution, for exploring the relationship between litter decomposition and soil carbon and nitrogen. Method Soil and litter in broad-leaved and coniferous forests were collected from subtropical forests. Six treatments were set, including litter, soil, litter + soil, nitrogen + litter, nitrogen + soil, and litter + soil + nitrogen. Three replicates were set for each treatment. The nitrogen addition amount was 120mg NH4 + -N·kg−1 soil. The amount of litter added in coniferous and broad-leaved forests was 12.1 g·kg−1 and 19.7 g·kg−1, respectively. The litter was placed on the surface of soil or quartzite, and the soil moisture was controlled at 60% water-holding capacity. An incubation experiment was carried out with nitrogen addition by leaching to simulate nitrogen deposition in a dark incubator at 25 ℃ for 220 days. During the incubation period, the nitrogen solution leached in 5 times with different nitrogen amounts, each with 110 mL of solution (80, 10, 10, 10, 10 mg NH4 + -N in sequence according to the number of leaching times), and the leaching solution was collected and measured.. In addition, the dissolved organic carbon (DOC) and nitrogen (DON), and NH4 + -N and NO3-N in the leaching solution were also measured. Result The results showed that the litter leaching solution had lower inorganic nitrogen and DON, and higher DOC. Litter addition significantly reduced the NO3-N by 22.6% and 29.9% in the coniferous forest and broad-leaved forest soil leaching solution, respectively and increased the DOC by 181.4% in the coniferous forest soil leaching solution. However, litter addition significantly decreased the DON by 39.2% in the broad-leaved forest soil leaching solution and MBN by 53.2% in the broad-leaved forest soil. Under nitrogen addition, the interception of added nitrogen by litter was less, and the interception of litter in broad-leaved forest litter was higher than that in coniferous forest. Litter input to soil by leaching decreased DOC, while DON increased. Nitrogen addition increased soil leaching inorganic nitrogen and coniferous forest soil leaching solution DON, but the effect of litter on reducing soil nitrogen leaching was not weakened by nitrogen addition. Conclusion The litter slows down the negative impact of soil NO3-N output on water environment. Nitrogen addition can affect soil nitrogen changes by altering the output of DOC and DON in broad-leaved and coniferous forest litters.
Larval Behavioural Responses of Melanotus cribricollis (Coleoptera: Elateridae) to the Volatiles Emitted from Bamboo Shoot and Carbon Dioxide
ZHANG Wei, TENG Ying, HUANG Xu-jun, LI Zhi-hong, ZHANG Ya-bo, SHU Jin-ping, WANG Hao-jie
2022, 35(6): 44-51.   doi: 10.13275/j.cnki.lykxyj.2022.006.005
[Abstract](2436) [FullText HTML](665) [PDF 807KB](15)
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Objective To understand the mechanism of food foraging below ground of Melanotus cribricollis larva which is the dominant and most destructive soil-dwelling insect of bamboo shoots in south China and provide important basic information for the development of effective monitor and control techniques. Method We tested the orientation behavior of M. cribricollis larvae towards bamboo shoot and CO2-releasing capsules by using a dual-choice olfactometer. The volatiles emitted from Phyllostachys violascens shoot were tentatively identified by solid phase micro-extraction (SPME) coupled with GC-MS, and their relative content were determined as well. Result Both bamboo shoot and CO2 source could significantly induce the directional movement of the larvae of M. cribricollis. There was no significant difference in the larval behavior among different weights of bamboo shoots (P>0.05), whiel larval orientation behavior was significantly correlated with CO2 concentration (P<0.05). Between bamboo and CO2-releasing capsules, wireworms preferred significantly to the bamboo shoot (P<0.05). A total of 17 compounds emitted from Ph. violascens shoot were tentatively identified. The higher relative contents were olefins (66.62%) and sesquiterpenes (27.98%), and the sesquiterpene compound with the highest content was α-humulene. Conclusion M. cribricollis larvae shows significant stronger orientation towards bamboo shoot and CO2 source, and prefers bamboo shoot significantly. Compared with CO2 source, the volatile organic compounds (VOCs) emitted from bamboo shoot show to be more effective in food foraging of bamboo shoot wireworm. In addition, 7 terpene compounds with potential attraction to wireworms are identified in this study, which provids important basic information for the development of attract-and-kill formulations for the control of wireworms.
The Effect of Russula rosea and Russula griseocarnosa on Microorganism Structure of Mycosphere Soil
YU Fei, LIANG Jun-feng
2022, 35(6): 52-63.   doi: 10.13275/j.cnki.lykxyj.2022.006.006
[Abstract](1570) [FullText HTML](898) [PDF 1524KB](47)
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Objective To provide a theoretical basis for the conservation and propagation of the two species of Russula and the realization of artificial cultivation, the mycosphere soil microbial communities of Russula rosea and Russula griseocarnosa was analyzed, and the soil microorganisms that promoting the growth of the two species of Russula were screened. Method The community structure of mycosphere and bulk soil microorganisms (fungi and bacteria) of two species of Russula were analyzed by Illumina MiSeq sequencing technology, and evaluated comprehensively based on soil physicochemical property. Result The mycosphere soil microorganisms of the two species of Russula were slightly different. The dominant mycosphere soil microorganisms of R. rosea were Acidothermus, Mycobacterium, Reyranella, Ktedonobacter, Rhodanobacter and Oidiodendron, while the dominant mycosphere soil microorganisms of R. griseocarnosa were Variibacter, Bryobacter, Blastochloris, Cladophialophora, Paecilomyces and Arthrinium. Bacteria (Variibacter, Candidatus_Solibacter, Sorangium, Mycobacterium, Singulisphaera, Isosphaera, Bdellovibrio and Paenibacillus) and fungi (Trichoderma, Penicillium, and Hypomyces) were the common growth promoting microbes of Russula rosea and R. griseocarnosa. pH was the key factor affecting mycosphere soil microorganisms. Conclusion The two species of Russula can specifically select soil microorganisms that are beneficial to their growth according to their growth needs and form different microbial community structures. The mycosphere mycorrhizal auxiliary bacteria that were screened out in this study can provide theoretical basis for the preparation of special microbial fertilizer and artificial propagation promotion of Russula, which has great promotion for the sustainable utilization of Russula, the expansion of understory edible fungi scale and the improvement of economic benefits.
Early Selection on Growth and Disease Resistance in Three Corymbia Species in Guangxi
LI Chang-rong, ZHENG Yong-deng, GUO Dong-qiang, QIN Kai-feng, CHEN Sheng-kan, LI Juan, TANG Qing-lan, WU Qin-zhan
2022, 35(6): 64-72.   doi: 10.13275/j.cnki.lykxyj.2022.006.007
[Abstract](616) [FullText HTML](293) [PDF 573KB](7)
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Objective To understand the growth of three Eucalyptus species of the genus Corymbia introduced in Guangxi, and preliminarily select species, provenances and families with disease resistance for providing scientific basis for the orient cultivation of sawn wood. Method Based on the 3 month-old provenance/family trail of Corymbia in Guangxi State Sanmenjiang Forest Farm, tree height and disease status were investigated. The disease resistance index was used to analyze the disease status., Differences between species, provenances, and families were evaluated by analysis of variance. Genetic variation parameters of traits were estimated, and the excellent species, provenances, and families for disease resistance were selected by independent elimination method. Results The results showed that: tree height, survival rate and resistance disease index had significant difference among four species (P < 0.001); there were no significant difference in height, survival rate and resistance disease index between provenances of Corymbia maculate and Corymbia henryi. The three traits were significant different among the families of these two provenances (P < 0.01), except that the disease resistance index of Corymbia maculate was not significantly different among families; there were extremely significant differences in tree height and disease resistance among the provenances and families of Corymbia citriodora ssp. variegate (P < 0.001), but the differences in survival rate were not significant; the difference of resistance disease index among provenances and families of Corymbia citriodora were significant (P < 0.001), but difference in height and survival rate were not significant. The heritability and repeatability ranged from 0.05 to 0.43, which was controlled by weak to moderate genetic intensity. Corymbia citriodora was the superior species with the best growth and disease resistance. A total of 7 provenances and 47 families were selected. Their mean values of height, survival rate and resistance disease index were 57.1%、1.8%、67.4% and 57.1%、0.9%、69.8% higher than the population mean values, respectively. The genetic gains of height and resistance disease index of provenance and family were 12.0%, 21.9% and 17.1%, 21.6%, respectively. Conclusion The disease-resistant species, provenances and families were selected, which provides basis of germplasm materials for further breeding.
Study on Adaptation Strategies of Quercus variabilis, Robinia pseudoacacia and Platycladus orientalis to Drought in Taihang Mountain
NIU Cun-yang, SHOU Wen-kai, YANG Xi-tian, ZHANG Zhi-hua, QIAO Yong-sheng
2022, 35(6): 73-82.   doi: 10.13275/j.cnki.lykxyj.2022.006.008
[Abstract](1763) [FullText HTML](727) [PDF 1539KB](22)
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Objective To provide theoretical basis for vegetation restoration in Taihang Mountain, the study discussed different adaptation strategies of Quercus variabilis, Robinia pseudoacacia and Platycladus orientalis to drought from perspective of hydraulic architecture. Method Based on Quercus variabilis (38-year-old), Robinia pseudoacacia (42-year-old) and Platycladus orientalis (30-year-old) in Taihang Mountain, Native sapwood specific hydraulic conductivity (Ks) and maximum specific hydraulic conductivity (Ksmax) of perennial stems were measured by using a Pipette. Percent loss of hydraulic conductivity (PLC) of Quercus variabilis and Robinia pseudoacacia were measured by flushing method, and PLC of Platycladus orientalis was measured by vacuum method. Wood anatomy was measured by using optical microscope and scanning electron microscope technology. Result (1) The mean vessel diameter (Dv), hydraulic weighted vessel diameter (Dh) and aperture fraction (Fap), number of pits per unit area of vessel wall (Np), total pit area per vessel area (Fa) of Quercus variabilis and Robinia pseudoacacia were all significant higher than those of Platycladus orientalis. Ks and PLC of the former two deciduous tree species were therefore significantly higher than those of Platycladus orientalis; (2) The Dv, Dh, surface area of inter-vessel pit membranes (Ap), area of single pit aperture (Aap), Fap, Np and Fa of Robinia pseudoacacia were significantly higher than those of Quercus variabilis. However, PLC of Robinia pseudoacacia was significantly lower than that of Quercus variabilis, which might be due to the fact that the pit chamber and pit aperture of Robinia pseudoacacia had punctate and dentate vestures, which enhanced resistance to drought induced cavitation. (3) The Aap , Fa and Fap increased with the increase in the diameter of the xylem vessels of the three tree species. Conclusion Quercus variabilis and Robinia pseudoacacia have high xylem water transport efficiency, which indicates that they employ fast growth strategy. While Platycladus orientalis adopts conservative resource acquisition strategy with lower xylem water transports efficiency. The xylem vessels and pit structures synergistically affect xylem water transport efficiency and resistance to drought-induced cavitation, and xylem anatomy is an important trait determining the adaptation strategies of different tree species to arid environments.
Parasitism Rate and Progeny Development of Sclerodermus alternatusi (Hymenoptera: Bethylidae) on Different Stage of Thyestilla gebleri (Coleoptera: Cerambycidae)
TANG Yan-long, WANG Li-na, JIA Jie-li, KANG Kui, ZENG Bo-ping, WEI Ke
2022, 35(6): 83-88.   doi: 10.13275/j.cnki.lykxyj.2022.006.009
[Abstract](602) [FullText HTML](405) [PDF 600KB](14)
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Objective To compare the differences of parasitism and progeny development of Sclerodermus alternatusi on different stages of Thyestilla gebleri. Method The parasitism rate, developmental duration, female and male progeny number, male progeny proportion of S. alternatusi parasitized the larva and pupa of T. gebleri were investigated. Result The results showed that both the larva and pupa of T. gebleri could be parasitized by the bethylid, and the parasitism rate on different stages of T. gebleri showed no significant difference. Successful emergence rate of parasitoid offspring showed significant difference on different stage of T. gebleri, 93.33% and 57.69% emergence rates were observed on the larva and pupa of longhorn-beetle. The pre-oviposition period of female wasp, egg stage, larval stage, pupal stage, and immature stage of wasps' offspring showed no significant differences on the larva or pupa of host, T. gebleri. The average pre-oviposition periods of female wasps were 6.63 d and 6.43 d when they were inoculated on the larva and pupa of cerambycid host, respectively. The average durations of offspring egg, larval, pupal, and immature stage were 4.34 d and 4.57 d, 8.34 d and 8.52 d, 19.89 d and 20.07 d, 32.61 d and 33.40 d, respectively. The number of female progeny and the total number of offspring were 39.50 and 41.18 when the parasitoid parasitized on the host larva, respectively; and the average numbers were 30.80 and 32.20 when the parasitoid were bred with host pupa. Both had significant differences. There was no significant difference in the number of male progeny when the foundress parasitized on host larva or pupa, with an average of 1.96 and 1.40. The male proportion was also not significantly different, with an average of 5.00% and 4.45%, respectively. Conclusion Emergence rate and female progeny quantity of parasitoid parasitized the T. gebleri larva are much higher than the beetle pupa was used as host. Using the late-stage larva of T. gebleri as alternative host can result in the highest fitness in the mass-rearing of S. alternatusi.
Genome-Wide Analysis of Microsatellite Loci and Specific Monomorphism SSR Marker Development in Walnut (Juglans regia L.) Reference Genome
HE Jun-xing, MA Qing-guo, PEI Dong, ZHANG Jun-pei
2022, 35(6): 89-100.   doi: 10.13275/j.cnki.lykxyj.2022.006.010
[Abstract](1664) [FullText HTML](709) [PDF 1248KB](35)
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Objective To identify the number and distribution of SSR loci on different chromosomes in the whole genome of walnut (Juglans regia L., 2n = 2x = 32), and to develop and validate the monomorphic SSR primers. Method In this study, walnut whole genome sequencing data were used as experimental materials, and the whole genome microsatellites were screened and analyzed by bioinformatics software MISA. Primer 3.0 was employed to design monomorphic SSR primers. SSR primers were evaluated by electronic PCR and some of the monomorphic ones were synthesized randomly to detect their usefulness and verify the effectiveness of the method. Result (1) A total of 357 629 SSR loci were identified in the walnut genome, with a distribution density of 662.28 SSRs/MB. The dominant repeat units were mainly A/T bases, showing significant base preference. These SSR sequences were mainly short sequences with a length of 10~30 bp, up to more than 95.00%. The number of SSR loci on different chromosomes varied greatly. Among them, the number of SSR loci on chromosome 1 was the largest, and the numer of SSR loci on chromosome 16 was the least. The number and type of SSRs showed positively correlated with chromosome sequence length. Most of the 644 rare SSR units were hexa-nucleotides. (2) Based on cluster analysis, all the 16 chromosomes could be divided into 4 groups, of which the number of members in group 4 was the most (11), and there was only chromosome 10 in group 1. In general, chromosome 10 forms a main branch, indicating that it may have experienced a relatively conservative evolutionary history. (3) 303 009 pairs of SSR primers were designed by using the conservative sequence flanking the SSR locus. And then 32 pairs of monomorphic primers clarified by electronic PCR were randomly screened and synthesized for wet PCR experiments, of which 30 pairs (93.75%) were amplified in 6 walnut varieties. The PCR amplification results of 28 pairs (87.50%) were consistent with that of electronic PCR. Conclusion In this study, SSR loci in different chromosome sequences of ‘Zhongmucha-1’ walnut reference genome are identified. Their amounts and repeat types are found to be highly variable among different chromosome sequences and show a highly significant positive correlation with chromosome length. Mono-nucleotide repeat SSRs are the most common type. A novel protocol combining electronic PCR and traditional screening methods are established and validated, which provide an effective strategy for the personalized and rapid development of walnut SSR primers. The developed 28 pairs of monomorphic primers can provide scientific basis for “Illegitimacy Testing” of hybrid offspring in molecular marker assisted breeding.
Synergistic Effect of 3-bromopyruvate Combined with Hyphantria cunea Nucleopolyhedrovirus Against Hyphantria cunea (Drury)
LIU Rui-xia, LI En-jie, BAI Chao, WANG Qing-hua, DUAN Li-qing
2022, 35(6): 101-107.   doi: 10.13275/j.cnki.lykxyj.2022.006.011
[Abstract](588) [FullText HTML](364) [PDF 591KB](18)
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Objective In this paper, we used 3-bromopyruvate (3-BrPA) as a biological synergist of Hyphantria cunea nucleopolyhedrovirus (HycuNPV) to investigate the effect of 3-BrPA on the virulence of HycuNPV and the growth and development of Hyphantria cunea, which will provide new synergist resources for the application of HycuNPV insecticides. Method The synergistic effect on HycuNPV was analyzed by adding different doses of 3-BrPA incorporating with HycuNPV in laboratory. Result The bioassay results showed that 3-BrPA had a synergistic effect on HycuNPV. It could not only improve infectivity of HycuNPV, but also increase the insecticidal speed. When the dosages of 3-BrPA with 5.0, 10.0 and 15.0 mg·g−1 were added to HycuNPV, the LC50 of HycuNPV in the 3rd instar larvae of H.cunea decreased. The synergistic ratio (SR) of HycuNPV were 825.14, 164.72 and 0.47, and the co-toxicity coefficients (CTC) were 82613.63, 16579.25 and 147.27, respectively. Different concentrations of HycuNPV had different insecticidal effects under the different doses of 3-BrPA. Added different doses of 3-BrPA, the LT50 of HycuNPV against the larvae were shorten by 3.280~9.724 d. According to SR, CTC, LT50 and ST, the optimum synergistic effect was observed: 5.0 mg·g−1 3-BrPA + 9.0 × 105 OBs·mL−1 HycuNPV. In addition, the combination of 3-BrPA and HycuNPV significantly inhibited the growth and development of larvae, and the pupation and emergence rate of surviving larvae were significantly lower than that of the virus insecticide alone. Conclusion 3-BrPA has a synergistic effect on HycuNPV, and is expected to be an ideal synergistic resource of viral insecticides.
Niche Characteristics of Population in Shrub Communities in Spruce-fir Forest Burned Areas on the Northeast Margin of Qinghai-Tibetan Plateau
ZHAO An, ZHOU Xiao-lei, SHI Rui-jin, ZHOU Xu-jiao, YANG Fu-qiang, HE Wan-peng
2022, 35(6): 108-117.   doi: 10.13275/j.cnki.lykxyj.2022.006.012
[Abstract](913) [FullText HTML](621) [PDF 2508KB](32)
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Objective To reveal the niche characteristics of shrub community population in the burned areas of Spruce-fir forest in different years on the northeast edge of Qinghai-Tibetan Plateau. Method Levins and Shannon Wiener niche breadth index, Pianka and Schoener niche overlap index were used to calculate the niche breadth and niche overlap degree of population, and the specialized species and generalized species of community were divided. Result The results of Levins and Shannon Wiener niche breadth index of shrub species in burned and unburned forest land were basically the same, and there were differences in the niche breadth of the same species in burned and unburned forest land. There are three specialized species in the 5-years burned area, two specialized species and 1 generalized species in the 15-years burned area and one specialized species in unburned forest land. The proportion of species logarithms with severe niche overlap among species was 15-year-burned land>5-year-burned land>unfired forest land. The species logarithm proportion of general niche overlap was 5-years-burned land>15-yearly-burned land>unfired Woodland. And the proportion of species logarithm with less niche overlap was unburned forest land > 15 years burned area > 5 years burned area. Conclusion After severe fire disturbance in the Spruce-fir forest on the northeast edge of Qinghai-Tibetan Plateau, the degree of niche overlap among species of shrub communities in burned areas of different years is constantly changing, while the degree of niche overlap among species in undergrowth of unburned forest land remains relatively low, and the community is in a positive succession stage.
Diversity of Endophytic Fungi from the Roots of Alpine Quercus in Subalpine Regions of Hengduan Mountains
LI Hong-bo, MA Li, HUANG Yao-hua, KANG Ding-xu, WU Jian-rong, MA Huan-cheng, CHEN Shi
2022, 35(6): 118-126.   doi: 10.13275/j.cnki.lykxyj.2022.006.013
[Abstract](715) [FullText HTML](427) [PDF 1398KB](14)
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Objective To understand the root endophytic fungal composition and diversity of alpine oaks in the Hengduan Mountains. Methods The root samples of three alpine oak species were collected. The fungal symbiosis within the root system was observed using the root immersion staining method, and the endophytic fungus were isolated and purified. These fungi species were identified by combining morphological and molecular biological methods. Results Numerous DSE fungi and AMF clumped structures were found in these oak roots samples. A total of 27 fungal species in 20 genera of 17 families were identified. one family of ectomycorrhizal fungus uncultured Ceratobasidiaceae (GSL1-7), four species of dark septate endophytes (DSE) Boeremia exigua var.exigua. Phialophora mustea (GSL2-2, GSL2-9), Paraboeremia putaminum (GSL4-F, GSL20-B), and two clearly reported plant growth-promoting fungi Pseudogymnoascus roseus (GSL4-1-A, GSL4-2) and Trichoderma tomentosum (GSL80-D) were also found. The consequence of α-diversity measurement indicated that diversity of culturable fungi in the root systems of three species of alpine oak was rich but not significantly different. Four species of DSE and three species of plant-promoting fungi accounted for 31.3% in the root system. Conclusion These fungi play an important role in the adaptive growth of alpine oak under the harsh environmental conditions in the Hengduan Mountains. The results of this paper provide a preliminary theoretical basis for elucidating the ecological adaptive mechanism of alpine oak in the Hengduan Mountains region, and provide a living strain resource for developing the use of probiotic fungi in alpine oak seedlings.
Influencing Factors on the Crown Ratio of Korean Pine Plantation in Eastern Liaoning Province
LIU Qi-feng, CHEN Dong-sheng, FENG Jian, GAO Hui-lin
2022, 35(6): 127-134.   doi: 10.13275/j.cnki.lykxyj.2022.006.014
[Abstract](1023) [FullText HTML](627) [PDF 844KB](28)
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Objective To study the effects of different competition levels and topography on the canopy length and compare the forest layers, the crown ratio model considering competition and topographic (slope, slope aspect and slope position) variables of Pinus koraiensis plantation in eastern area of Liaoning province was developed. Method The crown ratio model was developed by incorporating competition index and topographic variable using re-parameterization and dummy variable methods. and the specific topographic variable expressing the largest contribution was selected. The effect of competition and topographic variable on the crown ratio was simulated. Result Compared with the basic model, the R2a for the finial model incorporating competition, topographic variable and forest layer increased by 0.113, RMSE decreased by 0.012. The contributions of competition, slope aspect and forest layer to the crown ratio model were 14.14%、2.25%、0.16%, respectively. The crown ratio decreased with the increase of D and increased with the increase of forest competition. With the increase of the competition, the crown ratio turned from negative growth to positive growth with tree height increasing gradually . The F test showed that there were significant differences in crown ratio between different slope aspects and forest layers, and the change rule was shady slope > semi-sunny slope > sunny slope and upper forest layer > lower forest layer. Conclusion Topography and forest competition factors significantly affect the crown ratio, and slope aspect is the most important topographic factor affecting the crown ratio of individual tree of Pinus koraiensis plantation. The crown ratio model based on topography and forest competition has high accuracy and provides a reference for the management and growth prediction of Pinus koraiensis plantation in eastern area of Liaoning Province.
Effect of Different Concentrations of Graphene on the Growth, Root Morphology and 15N Uptake and Utilization of Cunninghamia lanceolata Seedlings
PENG Ting-ting, WEI Yong-ping, ZHANG Jin-feng, ZHANG Xiao, CHEN Hong-hua, CHEN Ai-ling, ZHAO Jian-guo, HU Ya-lin, CAO Guang-qiu
2022, 35(6): 135-142.   doi: 10.13275/j.cnki.lykxyj.2022.006.015
[Abstract](952) [FullText HTML](567) [PDF 1122KB](19)
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Objective To provide theoretical basis for the efficient cultivation of C. lanceolate, the effects of graphene on the growth, root morphology and nitrogen uptake and utilization of Cunninghamia lanceolata were investigated in this study. Method A pot experiment with four concentrations of graphene (0, 20, 25, 30 mg·L−1) was conducted in this study by using the superior clone of C. lanceolata seedlings ‘Yang 061’ to investigate the growth, root morphology, 15N distribution and utilization rate of C. lanceolata seedlings under various graphene treatments. Results The indexes of growth and root morphologies were maximum at 25 mg·L−1 graphene concentration except for stem weight and root volume, but no significant difference were observed among different treatments. The 15N utilization rate of seedlings was 25 mg·L−1>20 mg·L−1>30 mg·L−1>CK1, and the distribution of 15N in different organs of C. lanceolata seedlings was leaf > stems > roots. Correlation analysis results showed that a significantly positive correlation between roots 15N partition rate and seedlings 15N utilization rate with different growth indexes were observed except for root length, root volume, root 15N partition rate and root diameter. Conclusion A certain concentration of graphene is beneficial to the growth of C. lanceolata seedlings and can promote the absorption and utilization of N.
Analysis of Moth Diversity in Oak Forests, Quercus variabilis and Q. mongolica in Northern China
REN Xue-yu, PANG Yan, WANG Mei, LI Guo-hong, WANG Yan-jun, LI Jian-zhong, WANG Hong-bin
2022, 35(6): 143-150.   doi: 10.13275/j.cnki.lykxyj.2022.006.016
[Abstract](633) [FullText HTML](374) [PDF 960KB](14)
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Objective To provide the basis for monitoring and controlling moth pests in oak forests, the structure and diversity of moth communities were investigated in two main natural oak forest ecosystems, Quercus variabilis, and Q. mongolica, in northern China for revealing the potential changes caused by temporal and habitat. Methods The composition and diversity of moths in temporal and spatial differences were compared through collecting the specimen of moth using light trap during summer from June to September 2019 in three typical plots, including Q. variabilis forest in Jiulong Mountain, Mentougou District, Beijing; Q. mongolica forest in Luanping County, Hebei and in Keshiketeng, Inner Mongolia. Result A total of 293 moth species in 21 families were obtained from the plots. The largest number of moths and the most species were concentrated in Noctuidae and Geometridae. However, the species composition of the community differed significantly in terms of space and time. The moth species composition and structural similarity in the Q. mongolica forest in Hebei were more similar with the Q. variabilis in Beijing than those in Q. mongolica forest in Inner Mongolia. In addition, the emergence period of adult moths exhibited obvious temporal changes, and the greatest number of species and individuals were found in July at each plot. Diversity results showed that the Marglef richness index, Shannon diversity index, and Pielou evenness index of moths in Beijing and Inner Mongolia plots were higher than those in Hebei plot, and the community performance was stable. Among them, the Marglef richness index showed obvious differences in the diversity characteristics of family, genus, and species, while the changes of other indexes were consistent. Conclusion The composition and diversity of moth communities in oak forests show obvious spatio-temporal heterogeneity. Environmental factors can significantly affect moth biodiversity indicators. Richness indicator differences at various taxonomic levels can be used for distinguishing the geographical distribution and degree of certain taxa.
Comprehensive Evaluation of Antimony Tolerance of Different Catalpa bungei Clones based on Factor Analysis
LIU Zhen-hua, WANG Jun-hui, TONG Fang-ping, SUN liang, LI Gui, CHEN Rui, WU Min, LIU Zuo-qun, MA Wen-jun
2022, 35(6): 151-160.   doi: 10.13275/j.cnki.lykxyj.2022.006.017
[Abstract](568) [FullText HTML](300) [PDF 992KB](13)
Abstract:
Objective To evaluate the antimony tolerance of 10 different clones of Catalpa bungeiso for providing plant materials for the remediation of antimony contaminated soil. Method Under different antimony stress, the growth, biomass, leaf chlorophyll content, antimony content, leaf anatomical structure, hydrogen peroxide content, superoxide anion content, glutathione, and antioxidant enzyme activities (CAT, POD, APX) of different clones were determined. The antimony tolerance of 10 different clones was evaluated by factor analysis. Result The results showed that under different concentrations of antimony stress, there were significant differences in aboveground and root biomass except 8402. The plant height and ground diameter of most clones increased firstly and then decreased with the increase of sb concentration. The root biomass of clone 8402 and 72, was higher than that of the control. Under the sb concentration 2 000 mg·kg−1, the other clones promoted the increase of the root biomass under low antimony concentration, and gradually decreased with the increase of antimony concentration to a certain concentration. Antimony was mainly accumulated in root. Under concentration 600 mg·kg−1, clone 63 had the highest antimony content in roots and leaves, and 8402 had the highest antimony content in stems. Under 1 200 mg·kg−1, clone 5-2 had the highest antimony content in roots,1-1had the highest antimony content in the stem and leaf . Under 2 000 mg·kg−1, the highest antimony content in roots, stems and leaves was clone 5-8,8402 and 72, respectively. There was a positive correlation between the activity of antioxidant enzymes and the concentration of reactive oxygen species in plants under certain antimony stress. Conclusion The comprehensive evaluation of antimony tolerance of 10 Catalpa bungei clones under 0-2 000 mg·kg−1 antimony stress is clone 5-8 > 0 > 20-01 > 2-8 > 5-2 > 63 > 8402 > 1 > 72 > 1-1. The results provide plant materials for the remediation on antimony contaminated soil, and can improve the remediation effect of antimony contaminated soil. Under 2 000 mg·kg−1 antimony concentration in soil, Catalpa bungei clones with strong antimony tolerance can be used for remediation.
Effects of Slow-release Fertilizer Addition and Inoculation of Mycorrhizal Fungi on the Growth Traits and Nutrient Status of Cyclobalanopsis gilva Container Seedlings
HUANG Sheng-yi, YANG Meng-qing, WANG Bin, ZHOU Zhi-chun, WANG Xiu-hua, XU Wei-ke, WU Ren-chao
2022, 35(6): 161-169.   doi: 10.13275/j.cnki.lykxyj.2022.006.018
[Abstract](345) [FullText HTML](162) [PDF 699KB](10)
Abstract:
Objective To improve the container seedlings cultivation level of Cyclobalanopsis gilva with mycorrhiza. Method Based on the interaction experiments of different slow-release fertilizer addition levels and inoculation of mycorrhizal fungi, the effects of slow-release fertilizer × mycorrhizal fungi on the growth traits and nutrient status of 1-year-old container seedlings of Cyclobalanopsis gilva were analyzed. A split-plot experimental design was used with inoculation of mycorrhizal fungi in the main plot and different slow-release fertilizer addition levels in the sub-plot. The main plot was mycorrhizal treatment, which was divided into two treatments: inoculation (10 mL of Pisolithus tinctorius with a concentration of 36.28 mg·mL−1 was injected into the middle part of the seedling container) and non inoculation. The area was treated with matrix slow-release fertilizer addition, and five slow-release fertilizer addition gradients (1.50, 2.25, 3.00, 3.75, and 4.50 kg·m−3) were set. Results Inoculation of mycorrhizal fungi promoted the growth of 1-year-old C. gilva container seedlings under different slow-release fertilizer addition levels. There was significant interaction between slow-release fertilizer addition and inoculation of mycorrhizal fungi on total biomass and P utilization index of container seedlings. After inoculation with mycorrhizal fungi, growth traits such as seedling height, caliper, sturdiness quotient, total biomass, total root length and root diameter increased by 18.70%, 7.16%, 10.75%, 24.55%, 1.02% and 18.47% compared with that no inoculation, respectively. N content, N uptake and utilization index, P uptake and utilization index increased by 2.04%, 27.10%, 27.67%, 19.72% and 30.52%, respectively, while P content decreased by 2.94%. There were extremely significant differences in growth traits and nutrient status between inoculated and non-inoculated except total root length and N and P contents. Regardless of whether mycorrhizal fungi were inoculated or not, with the increase of slow-release fertilizer addition level, all growth traits increased first and then decreased except root diameter, P content and uptake showed an overall increasing trend. The growth was the best when the slow-release fertilizer addition level was 3 kg·cm−3. The N content and absorption of the container seedlings without mycorrhizal inoculation showed a trend of increasing first and then decreasing, while the N and P utilization indexes decreased first and then increased. There was no obvious change in N content and N absorption. Conclusion Inoculation of ectomycorrhizal fungus with P. tinctorius can promote the growth of C. gilva container seedlings and improve the nutrient utilization efficiency of N and P, and the effect of promoting mycorrhizal growth is more significant under low slow-release fertilizer addition.. In production, inoculation of mycorrhizal fungi can improve the quality of container seedlings and reduce the use of slow-release fertilizer, which is helpful to the cultivation of high quality seedlings of C. gilva and save seedling cost.
Radial Variation of Sap Flow in Eucalyptus and Its Effect on the Estimation of Whole Tree Transpiration
QIN Jia-shuang, GU Da-xing, NI Long-kang, HE Wen, LIN Fu-rong, ZHOU Cui-ming
2022, 35(6): 170-176.   doi: 10.13275/j.cnki.lykxyj.2022.006.019
[Abstract](531) [FullText HTML](335) [PDF 942KB](18)
Abstract:
Objective To provide a reliable basis for the accurate estimation and scale expansion of transpiration in medium and large diameter Eucalyptus plantations, the radial variation of sap flow and its effect on the estimation of whole tree transpiration were analyzed. Methods The sap flow density at three sapwood depths (0-2 cm, 2-4 cm and 4-6 cm) of 10-year-old Eucalyptus urophylla × Eucalyptus grandis was monitored using the Granier-type thermal dissipation probe (TDP). Result The results showed that the diurnal variation of sap flow density in each sapwood depth showed a single-peak pattern, and the sap flow density decreased with the increase of sapwood depth. Compared with the daily whole tree transpiration estimated based on sap flow density in three sapwood depths (Tr246), the daily transpiration when trade the sap flow density in 0-2 cm (Tr2), 2-4 cm (Tr4) and 4-6 cm (Tr6) sapwood depth as the whole tree sap flow density were overestimated by 92.9% and underestimated by 28.2% and 74.0%, respectively. The sap flow in 0-2 cm sapwood depth was more sensitive to environment compared with the other two depths and Tr2 had higher correlation with Tr246 (R2=0.964) compared with Tr4 and Tr6. As a result, the sap flow in 0-2 cm depth could be used to estimate whole tree transpiration of Eucalyptus when calibrated based on Tr246 (Tr246=0.522 Tr2). Conclusion Eucalyptus shows obvious radial variation in sap flow density and there may lead to large errors in estimating whole tree transpiration when ignoring the radial variation of sap flow. The whole tree transpiration of Eucalyptus can be estimated easily and accurately using sap flow in 0-2 cm sapwood depth based on the calibrated function.
Effects of Environment-Friendly Water-retaining Materials on Soil Moisture Characteristics of Soil and the Evaluation
HOU Mei-juan, WEI A-long, CONG Ri-chun
2022, 35(6): 177-186.   doi: 10.13275/j.cnki.lykxyj.2022.006.020
[Abstract](626) [FullText HTML](358) [PDF 1508KB](14)
Abstract:
Objective To explore the influence of environment-friendly water-retaining materials on soil moisture characteristics. Methods Three environment-friendly water-retaining materials, porous fiber cotton, biochar and carbon cotton, were selected and compared with three traditional water-retaining materials, sodium polyacrylate, acrylamide-potassium acrylate and potassium polyacrylate. Sandy soil was taken as the matrix and mixed with various water-retaining materials at different mass ratios, and CK. By the method of water control experiment, the dynamic curves of soil moisture and soil water characteristic of each water-retaining material were measured, respectively. And the influence of water-retaining materials on soil water characteristics was analyzed. Results The environment-friendly water-retaining materials could reduce soil bulk density and increase soil porosity and water holding capacity. Among them, 10% carbon cotton in soil had a significant effect on the water holding parameters. Under the condition of natural drought, all water-retaining materials could restrain soil water evaporation more effectively than CK. During 40 days, 10% carbon cotton retained the most moisture throughout the water loss process. After 40 days of drought stress, the water content of 10% cotton was 3.37% higher than that of CK group. All of the six water-retaining materials could increase the soil effective water content. The soil effective water content in the material T3 had the largest, followed by N, H, T2, S3, S2, D3, K, D2, S1, T1, D1, CKof which 10% cotton treated soil effective water content was the most. Conclusion Through the comprehensive evaluation of principal component analysis and membership function method, 10% carbon cotton has the best water retention effect among environment-friendly water retention materials.
Advances in Tissue Culture Techniques of Trees and the Problems Existed
HUANG Lie-jian, WANG Hong
2016, 29(3): 464-471.  
[Abstract](3288) [PDF 914KB](1608)
Effect of Thinning on Growth and Timber Outturn in Cunninghamia lanceolata Plantation
XU Jin-liang, MAO Yu-ming, ZHENG Cheng-zhong, FAN Rong-de, ZHOU Shi-shui, CHEN Yong-hui, CHENG Xiang-rong, YU Mu-kui
2014, 27(1): 99-107.  
[Abstract](3444) [PDF 992KB](1432)
On the Maintenance of Long-term Productivity of Plantation in China
SHENG Wei-tong
2018, 31(1): 1-14.   doi: 10.13275/j.cnki.lykxyj.2018.01.001
[Abstract](4494) [FullText HTML](1474) [PDF 1291KB](950)
Comparison on Osmotica Accumulation of Different Salt-tolerant Plants under Salt Stress
CHENG Tie-long, LI Huan-yong, WU Hai-wen, LIU Zheng-xiang, WU xiang, YANG Sheng, ZHANG Hua-xin, YANG Xiu-yan
2015, 28(6): 826-832.  
[Abstract](3261) [PDF 1605KB](1110)
Research Progress of Structure-based Forest Management
HUI Gang-ying, HU Yan-bo, ZHAO Zhong-hua
2018, 31(1): 85-93.   doi: 10.13275/j.cnki.lykxyj.2018.01.011
[Abstract](5527) [FullText HTML](2102) [PDF 2383KB](776)
Quantitative Analysis of Forest Spatial Structure and Optimal Species Composition for the Main Forest Types in Daxing'anling, Northeast China
DONG Ling-bo, LIU Zhao-gang, LI Feng-ri, JIANG Lei
2014, 27(6): 734-740.  
[Abstract](3836) [PDF 1059KB](1209)
Growth and Structure Differentiation of Phoebe bournei Plantation with Different Sites and Modes of Afforestation
CHU Xiu-li, LIU Qing-hua, FAN Hui-hua, WANG Sheng-hua, CHEN Liu-ying, ZHOU Zhi-chun
2014, 27(4): 445-453.  
[Abstract](3338) [PDF 1443KB](1365)
Stoichiometry Characterization of Soil C, N and P of Pinus massoniana Plantations at Different Age Stages
LEI Li-qun, LU Li-hua, NONG You, MING An-gang, LIU Shi-ling, HE Yuan
2017, 30(6): 954-960.   doi: 10.13275/j.cnki.lykxyj.2017.06.010
[Abstract](3197) [FullText HTML](365) [PDF 1293KB](749)
Studies on Seedling Photosynthetic Characteristics of Five Tree Species under Drought Stress
HAN Bo, LI Zhi-yong, GUO Hao, ZHANG Jun-pei
2014, 27(1): 92-98.  
[Abstract](3123) [PDF 1078KB](1258)
Effect of Close-to-Nature Management on Species Diversity in a Cunninghamia lanceolata Plantation
SUN Dong-jing, WEN Yuan-guang, LUO Ying-hua, LI Xiao-qiong, ZHANG Wan-xing, MING An-gang
2015, 28(2): 202-208.  
[Abstract](3084) [PDF 1225KB](1306)

Journal Information

Publication name:林业科学研究 Forest Research

Editor:ZHANG Shou-gong

Sponsors by:Chinese Academy of Forestry Sciences

Address:Chinese Academy of Forestry after Beijing Wanshou Mountain

Telephone:010-62889680;62889702

E-mail:lykxyj@caf.ac.cn

WebSite:http://www.lykxyj.com

ISSN 1001-1498

CN 11-1221/S

Postal code:80-717

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