• 中国中文核心期刊
  • 中国科学引文数据库(CSCD)核心库来源期刊
  • 中国科技论文统计源期刊(CJCR)
  • 第二届国家期刊奖提名奖

Journal Introduction

Forestry Science Research is a comprehensive academic journal of forestry science sponsored by the Chinese Academy of Forestry Science. The main task is to timely reflect the latest research results, academic papers and reports, scientific and technological trends and information of forestry science with the Chinese Academy of Forestry as the main body, to promote academic exchanges at home and abroad, to carry out academic discussions, to prosper forestry science and to better serve China's forestry construction. The main contents are: forest seeds, seedling raising and afforestation, forest plants, forest genetic breeding, tree physiology and biochemistry, forest insects, resource insects, forest pathology, forest microorganisms, forest birds and animals, forest soil, forest...

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Articles in press have been peer-reviewed and accepted, which are not yet assigned to volumes /issues, but are citable by Digital Object Identifier (DOI).
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Three-stage Tree Height Inversion Algorithm with Compensation for Temporal Decorrelation
Xiao LI, Zeng-yuan LI, Er-xue CHEN, Jia-zhou CHENG, You-yi JIANG
 doi: 10.13275/j.cnki.lykxyj.2020.03.011
[Abstract](166) [FullText HTML](135) [PDF 775KB](5)
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Objective When polarimetric SAR data are used to invert tree height, time decorrelation factor is the main factor affecting inversion accuracy. Random-Motion-over-Ground (RMoG) model is one of the most effective models, but it has the disadvantages of difficult inversion and long time-consuming. Here, a simplified RMoG model is proposed. Method In this study, the ground motion was neglected, the vegetation canopy motion was retained, and the vegetation volume scattering formula was rewritten. Then, the ground phase was judged by linear fitting of multiple coherence coefficients, and the decoherence value of pure volume scattering was estimated by PD polarization coherence optimization method. Finally, the rewritten vegetation volume scattering formula was used to establish a survey. Based on the fixed extinction coefficient, the height of vegetation can be retrieved by looking-up table. To verify the validity of this method, the remote sensing data of BioSAR 2007 project were tested in Remingstorp, southern Sweden. The inversion results of the four models were compared and evaluated with the determination coefficient (R2) and the root mean square error (RMSE). Result This method can improve the overestimation problem of three-stage algorithm. In terms of accuracy comparison, the R2 of three-stage algorithm is 0.78 and RMSE is 8.52; the R2 of RMoG model is 0.47 and RMSE is 4.17; the R2 of RMoGL model is 0.48 and RMSE is 2.50; the R2 of this method is 0.53 and RMSE is 6.24. It is showed that this method is better in accuracy compared with three-stage algorithm, and can effectively reduce the inversion time compared with RMoG model and RMoGL model. Conclusion It is effective to eliminate time-related effects by adding vegetation canopy movement. Compared with three-stage algorithm, RMoG model and RMoGL model, the simplified RMoG model has the advantages of high accuracy and less time-consuming.
Effects of Nitrogen Fertilization on Early Growth of Betula alnoides Clones
Lin CHEN, Ji ZENG, Sheng-jiang PANG, Zhi-gang ZHAO, Hong-yan JIA
 doi: 10.13275/j.cnki.lykxyj.2020.03.009
[Abstract](335) [FullText HTML](263) [PDF 506KB](11)
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Objective To examine the response of early growth of Betula alnoides Buch.-Ham. ex D. Don to nitrogen application and reveal the differences in the nitrogen requirement among clones. Method Four clones of Betula alnoides were used as materials in the nitrogen fertilization (urea) experiment with seven nitrogen treatments (0, 50, 150, 250, 400, 550 and 700 g urea per seedling), and the indicators such as survival rate, growth (tree height, DBH, height to crown base and crown width) and stem form (DBH/H ratio, axis persistence and stem straightness) were investigated, respectively. This study is to determine the differences in these traits among nitrogen fertilization treatments, clones and their interactions. Result The survival rate showed no obvious change, while the tree height, DBH and crown width displayed an “increase-stabilize-decrease” trend with the increase of nitrogen application amount for all the four clones in five years after planting. Moreover, the DBH/H ratio, axis persistence and stem straightness also tended to be better with the increasing nitrogen application. However the differences between nitrogen fertilizer treatments did not reach a significant level (P>0.05). There were significant differences among clones in the growth and stem form traits. The clone C3 showed the greatest performance, whose survival rate, tree height, DBH, height to crown base, crown width, axis persistence and stem straightness were 34%, 42%, 32%, 16%, 25%, 22% and 19% higher than those of clone C2, respectively. The interaction between nitrogen fertilization and clone was not significant. Conclusion Nitrogen fertilization would not affect the early afforestation growth and stem form quality of Betula alnoides. The clone C3 showed the best growth and wood quality.
The Differences of Soil Enzyme Activity and Quality Evaluation of Cunninghamia lanceolata Large-diameter Industrial Plantation under Different Understory Vegetation Management Measures
Guang-qiu CAO, Yu-chong FEI, Jin LU, Ying HUANG, Hong ZHENG, Kai-min LIN, Chun-shan JI, Shi-jiang CAO
 doi: 10.13275/j.cnki.lykxyj.2020.03.010
[Abstract](563) [FullText HTML](994) [PDF 663KB](7)
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Objective To study the effects of different understory vegetation management measures on soil enzyme activities and quality in Cunninghamia lanceolata large-diameter industrial plantation. Method Taking Cunninghamia lanceolata large-diameter industrial plantation as the research object, This study analyzed soil enzyme activities of plantation under three kinds of understory vegetation management measures, i.e. understory vegetation protection (UP), understory vegetation removal (UR) and understory interplanting (IP). The integrated quantitative evaluation on soil quality was made by principle components analysis taking soil enzymes as soil biological activity indexes combining with soil physical and chemical characteristics. Result The IP increased the sucrase activity in the soils with the depth of 0-20 cm, and there was no obvious difference between the sucrase activity in the soils with the depths of 20-40 and 40~60 cm under these understory vegetation management measures. Compared with UR and IP, the UP showed better effect in increasing soil catalase activity and decreasing soil urease activity, and the soil urease and catalase activity under UR and IP showed no obvious difference. The ranking of soil acid phosphatase activity under the three kinds of understory vegetation management measures was IP>UR>UP, and the ranking of soil polyphenol oxidase activity was UP>IP>UR. The activities of urease, sucrase and polyphenol oxidase in soil under different understory vegetation management measures showed a big difference, and the response of polyphenol oxidase to understory vegetation management measures was more sensitive and was reflected in deeper soil layer. Except acid phosphatase activity, the activities of other soil enzyme showed a obvious property of surface-aggregation, and decreased with the deepening of soil layer. The contents of organic matter and hydrolyzed nitrogen showed extremely significant or significant positive correlation with the activities of the 5 kinds of soil enzyme, the content of available phosphorus showed extremely significant positive correlation with the activities of sucrase, urease and acid phosphatase, and the content of available potassium showed extremely significant positive correlation with the activities of sucrase, catalase and polyphenol oxidase, the activities of soil enzymes were less dependent on the soil physical properties. Soil enzyme activity was taken as one of soil quality indexes, combining with soil physical and chemical characteristics, three principal components were extracted through principal component analysis, reflecting 75.31% of the original information amount. The ranking of soil quality index among different understory vegetation management measures was IP>UP>UR. Conclusion To sum up, in the large diameter Cunninghamia lanceolata plantation, the effect of planting Phoebe bourmei under the plantation is the best to maintain and improve the soil quality, followed by protecting understory vegetation, and the effect of understory vegetation removal is poor.
Effects of Uneven-aged Cunninghamia lanceolata and Evergreen Broadleaved Mixed Plantations on Soil Aggregate Stability and Soil Organic Carbon and Nutrients Stocks
Hai-dong XU, Hai-jing YUAN, Jing XIONG, Mu-kui YU, Xiang-rong CHENG
 doi: 10.13275/j.cnki.lykxyj.2020.03.014
[Abstract](151) [FullText HTML](147) [PDF 1034KB](9)
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Objective To study the effect of uneven-aged mixed plantations (Cunninghamia lanceolata + evergreen broadleaved tree species) on soil aggregates stability, organic carbon and nutrient storage, so as to provide references for optimizing the plantation structure and improving the sustainable forest management. Method This study investigated the uneven-aged mixed and the monoculture C. lanceolata plantations in Kaihua County of Zhejiang Province, and analyzed the stability of water-stable aggregates (WSA) and the changes of soil organic carbon (SOC), total nitrogen (TN), total phosphorus (TP) and magnesium (Mg) stocks in 0~20 cm soil layer. Result (1) The proportion of water-stable micro-aggregates (size<0.25 mm, WSA<0.25 mm) was the highest in the uneven-aged mixed and monoculture plantations. Compared with the monoculture plantation, the >5 mm aggregates proportions (WSA>5 mm), the soil mean weight diameter (MWD) and geometric mean diameter (GMD) significantly increased in the uneven-aged mixed plantations, but the soil fractal dimension (D) reduced. (2) The changes of SOC and TN stocks in different aggregate size of the uneven-aged mixed and the monoculture plantations were similar: WSA>5 mm, WSA2~5 mm>WSA0.25~2 mm>WSA<0.25 mm. The stocks of TP and Mg less varied in different aggregate sizes. (3) The stability of aggregate was mainly affected by WSA>5 mm and SOC>5 mm contents, pH, SOC2~5 m. bulk soil and soil aggregates organic carbon and nutrient stocks were mainly affected by SOCA>5 mm, 2~5 mm nitrogen contents (TNA2~5 mm) and pH. (4) Tree species composition in the uneven-aged mixed plantations greatly affected on aggregate composition and stability, SOC and nutrient stocks. The results of principal component analysis (PCA) showed that C. lanceolata+Phoebe sheareri significantly affected TP and Mg stocks in bulk soil and soil aggregates, and also affected MWD and GMD; C. lanceolata+Illicium henryi significantly affected SOC and TN stocks in bulk soil and soil aggregates. Conclusion In general, the establishment of uneven-aged C. lanceolata and broadleaved evergreen mixed plantations is beneficial to improve the soil physical and chemical properties of monoculture plantation, especially the introduced tree species of Ph. sheareri and I. henryi greatly enhance soil aggregate stability, soil organic carbon stocks and nutrient stocks of monoculture plantations.
Influences of Prescribed Burning on Regional Forest Burning Probability
Xue-zheng ZONG, Xiao-rui TIAN, Heng TIAN, fang CHEN
[Abstract](233) [FullText HTML](80) [PDF 957KB](10)
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Objective Burning probability model was used to simulate fire burning on the landscape scale and the impacts of prescribed burning on forest flammability were assessed quantitatively. Method The data about fuel conditions of some areas of Daxing’anling after prescribed burning before the fire season of 2016 were extracted from SPOT6 satellite data. The daily weather indices in the fire season were calculated with the weather observation data near the study area by using R-software. The burning probability and fire behavior indices of the forest were simulated with BURN-P3 model for the scenarios with and without prescribed burning. Result The areas treated with prescribed burning in 2016 were 44,931 hm2, accounting for 20.8% of the total area. The average burning probability was 0.0164 and 0.0124 for the scenarios with and without prescribed burning. The average burning probability for each fuel type respectively decreased by 4.2% for deciduous coniferous forest, 3.5% for evergreen coniferous forest, 5.9% for coniferous and broad-leaved mixed forest, 2.3%for grass, and 0.6% for broad-leaved forest after prescribed burning. The average fire intensity and spread speed were 548.9 kW/m2 and 2.2 m/min under the scenario with prescribed burning. The average fire intensity and spread rate decreased by 17.9% and 24.3% due to prescribed burning. Deciduous coniferous forests and mixed forest showed high fire intensity and spread speed, and are easy to occur crown fires. The proportion of crown fire reduced by 11.7% after prescribed burning. Conclusion The prescribed burning could decrease the distribution areas of grass fuel and fuel loadings. The burn probability, fire intensity, rate of spread and crown fire fraction of the region decrease after prescribed burning. The burning probability will drop obviously in the buffer areas with 2500 m wide around the prescribed burning regions.
Dynamic Visual Simulation of Stand Multi-objective Management Based on Simulated Annealing Algorithm
Kang SHEN, Ting-dong YANG, Huai-qing ZHANG, Hong ZHANG, Nian-Fu ZHU, Hua LIU
[Abstract](427) [FullText HTML](493) [PDF 1173KB](23)
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Objective Based on simulated annealing algorithm, a multi-objective forest management method considering the health status of stand structure and average diameter of stand was studied. The individual tree growth equation was used to realize the dynamic visualization of the stand management based on the Unity3D visualization engine. Method Based on 2012-2017 survey data of 5 Chinese fir (Cunninghamia lanceolata) sample stands located in Huangfengqiao State-owned Forest Farm of You County, Hunan Province, considering the distribution status, competition degree and nutrient space of the stand, the spatial structure function was established by three structural parameters: average angular scale, average size ratio and forest congestion degree. On the basis of spatial structure function, taking the healthy forest structure and the average diameter as management objectives, taking the results of a combinatorial permutation of thinned trees as the set of management planning with competition index as reference, the simulated annealing algorithm was used to select the optimal thinning mangement planning. Finally, by establishing a individual trees growth equation considering the variable growth rate of Hegyi competition index, the structural analysis-management-growth method was continuously iterated and the dynamic visualization of the forest management based on Unity3D rendering engine was realized. Result The dynamic visualization of the management dynamics was carried out on the plot 5 (initial stand of 230 trees, 16-years-old, average diameter 20.21 cm) with the healthy stand structure and average diameter of 30 cm as target. The stand was simulated thinned in the 21st, 26th, and 31st years. When the forest was 31 years old (after thinning), the average diameter reached 30.10 cm, the average angular scale of the forest was 0.65, and the forest congestion was 0.74, which showed a health level. Conclusion The dynamic visual simulation method of forest multi-objective management based on simulated annealing algorithm proposed in this paper can simulate forest dynamics and meet the multi-objective management demand. The image is intuitive, and can support research and production.
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Monomer Structural Stability of a Tau Class Glutathione Transferase (PtGSTU1) from Pinus tabulaeformis
Xiao-xia WANG, Hai-ling YANG, Jian-feng MAO, Xiao-ru WANG
2020, 33(2): 1-8.   doi: 10.13275/j.cnki.lykxyj.2020.02.001
[Abstract](660) [FullText HTML](168) [PDF 5858KB](49)
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[Objective] To study the relationship between structure stability and function of PtGSTU1. [Method] Homology modeling was used to simulate the three-dimensional structure of PtGSTU1. It is proposed that arginine (Arg18) in N-terminal domain and aspartic acid 103 (Asp103) in C-terminal domain are capable of forming a hydrogen bond to stabilize the protein monomer structure. Arg18 and Asp103 were mutated to amino acid residues with different polarities and conformations, respectively, using site-directed mutagenesis, and the catalytic activity and structural stability of the mutants were examined. [Result] None of all the six Arg18 mutants could obtain high-purity soluble protein with the correct folding, while Asp103 mutant could be expressed as a soluble protein whose catalytic activity and structure stability to different substrates were significantly lower than those of the wild type. The catalytic rate ( V max) of Asp103 mutant to classical substrates CDNB and GSH was reduced by 8/9 at least, and the catalytic efficiency ( k cat / K m) to the substrate was also significantly reduced. [Conclusion] The results proves that as the C-terminal domains are more variable than N-terminal domains in plant GST proteins, there may be some other amino acid sites in the C-terminal domains that can form hydrogen bonds with the arginine to stabilize the protein monomer.
Responses of δ13C Value of Cellulose in Tree Ring of Chinese Fir Provenances to Annual Climate Factors
Xiao-ying LIU, Ai-guo DUAN, Jian-guo ZHANG, Xiong-qing ZHANG, An-ming ZHU
2020, 33(2): 9-18.   doi: 10.13275/j.cnki.lykxyj.2020.02.002
[Abstract](677) [FullText HTML](351) [PDF 895KB](31)
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Objective Trying to explain the response of Chinese fir (Cunninghamia lanceolata) provenances to various climatic factors by analyzing the correlation between stable carbon isotope value in the cellulose of Chinese fir and the climatic factors. Method The δ13C value of cellulose of 30 provenances was extracted and measured from the experimental forest farm of Chinese fir in Liuzhou, Guangxi. The δ13C value, the tree ring, the precipitation, the mean temperature, the minimum air temperature, the maximum air temperature, the mean relative humidity, the minimum relative humidity, the sunshine hours and the humidity index in the current year, the first year and the first two years were analyzed. Result The variation range of δ13C value of tree ring of different Chinese fir provenances during 1987—2013 was -20.997‰--25.910‰, showing strong first-order autocorrelation, the correlation coefficient was 0.181-0.866. The δ13C value of tree ring of different Chinese fir provenances was significantly and positively correlated with the mean temperature, the maximum temperature and the minimum temperature (p<0.05), and positively correlated with sunshine hours. There was a significantly negative correlation between the δ13C value and the mean and minimum relative humidity (p<0.01), but no obvious correlation with the precipitation and wetness index. The results of stepwise regression analysis showed that 26 out of the 30 Chinese fir provenances had linear relationship with the minimum relative humidity of the current year. Conclusion The minimum relative humidity is the dominant climatic factor affecting the δ13C value of cellulose in the tree ring of Chinese fir provenances, which has little dependence on the precipitation. The response of δ13C in Chinese fir tree ring to climate factors has obvious lag effect. The study may provide reference for the cultivation and management of Chinese fir.
Genetic Diversity Analysis of Cotoneaster buxifolius in Yunnan Province Based on Phenotype Characters and ISSR Marker
Yan-lei ZHU, Feng-gen GUO
2020, 33(2): 19-26.   doi: 10.13275/j.cnki.lykxyj.2020.02.003
[Abstract](329) [FullText HTML](108) [PDF 688KB](22)
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Objective To reveal the level of genetic diversity of wild populations of Cotoneaster buxifolius Lindl. from the viewpoints of phenotypic traits and molecular marker. Method 33 phenotypic traits and ISSR primers were used to investigate the genetic diversity of wild populations of C. buxifolius in Yunnan. The diversity indices were calculated by PopGen software and the phylogenetic trees were constructed by SPSS 16.0 software. Result The analysis of numeric traits indicated that the average coefficient of variation of leaf length and leaf width was 25.99%. The variation of three related traits of calyx lobes was 40.66%, and the difference was significant. In total 228 bands were detected by 24 ISSR primers, 153 of them were polymorphic and the percentage of polymorphic bands was 81.56%. The Nei’s gene diversity index was 0.352 0 while the Shannon diversity index was 0.505 9. These data indicated that there was abundant genetic diversity in C. buxifolius. The 2 phylogenetic trees based on phenotypic traits and ISSR markers by UPGMA were similar. Most populations in the same distribution areas were classified into the same group, while a few populations did not combine with other populations in the same distribution areas. This phenomenon indicated that the geographical isolation promoted genetic differentiation and there were convergent evolutions due to the similar micro-habitat. Conclusion The C. buxifolius in Yunnan shows the higher level of genetic diversity. The inter-specific relationship and distribution of C. buxifolius populations are closely related to geographical location and altitude.
Effect of Extreme Snowfall on Soil Respiration of Plantations in North Subtropics-Warm Temperature Transition Zone
Zhi-man ZAN, Yan-chun LIU, Yin-zhan LIU, Juan XUAN, Wei ZHAO
2020, 33(2): 27-34.   doi: 10.13275/j.cnki.lykxyj.2020.02.004
[Abstract](280) [FullText HTML](130) [PDF 882KB](23)
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Objective To study the effect of extreme snowfall on soil respiration of plantations in north subtropics-warm temperate transition zone. Method A controlled experiment including snow addition, natural snowfall and snow remove was conducted in a plantation after a heavy snowfall in January 2018 in Xinyang, He'nan Province. The soil respiration variations under different treatments were measured with LI-8100 in different snow cover depths. The soil temperature, soil moisture, microbial biomass carbon, microbial biomass nitrogen, and soil available nitrogen contents were detected to determine the relationships between soil respiration and environmental factors. Result The results showed that the snow addition significantly elevated soil temperature in the earlier stage of the experiment. However, the soil temperature under snow addition treatment was significantly lower than that in the control during the middle and later stage, and across the whole experiment, respectively. Snow addition elevated the soil respiration rate by 21.57%, but snow remove did not affect the soil respiration. The change of snowfall did not affect the contents of microbial biomass carbon, microbial biomass nitrogen and soil available nitrogen. The elevated soil respiration was mainly ascribed to the elevation of soil temperature in the early stage of the experiment. Conclusion Extreme snowfall may increase soil respiration rate in plantations in the climate transitional zone, but it is affected by snowfall. The snowfall with the depth of about 30 cm will not significantly affect soil respiration rate. If snow depth continues to increase, the soil carbon emission rate will increase. In addition, the impact of snow depth on soil respiration at different stages of snowmelt is not consistent, and the impact of snow on soil respiration mainly occurs before the snow melts completely. This study can provide partial data supporting the establishment of ecosystem carbon cycle model under climate change scenario.
Effect of Ramet Age on Nitrogen Clonal Integration of Phyllostachys violascens Based on Stoichiometric Characteristics of C, N and P
Chao ZHANG, Rui GU, Shuang-lin CHEN, Jun-shuai SHI, Zi-wu GUO, Jun LIU, Qi-jiang HE
2020, 33(2): 35-42.   doi: 10.13275/j.cnki.lykxyj.2020.02.005
[Abstract](364) [FullText HTML](432) [PDF 547KB](20)
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Objective To study the response mechanism of carbon (C), nitrogen (N) and phosphorus (P) stoichiometric characteristics of different age ramets of Phyllostachys violascens to nitrogen clonal integration, and to explore the suitable age of bamboo for fertilization, so as to provide references for precise and reduced fertilization in Ph. violascens forest. Method The clonal integration unit consisting of 1-year-old and 2-year-old ramets of Ph. violascens was studied. Three nitrogen addition levels (4.07 mol N·L−1; 8.13 mol N·L−1; 12.20 mol N·L−1) were set up by injecting 15NH415NO3 into the bamboo cavity of different age ramets. The contents of C, N and P in leaves of 1-2-year-old bamboo were measured on time gradient, and the differences of N integration direction and transmission efficiency among ramets of different ages were discussed. Result Clonal ramets of Ph. violascens showed strong N conduction function. 1-year-old bamboo partly transmitted N to 2-year-old bamboo, while 2-year-old bamboo transferred N to 1-year-old bamboo as much as possible. Therefore, the N transmission capacity of 2-year-old bamboo was significantly stronger than that of 1-year-old bamboo. In addition, it showed time effect and N concentration effect to some extent. Low or high N concentration weakened the N transmission capacity. The middle N concentration in the experiment showed an "inflection point" of significant changes in the integration function. Nitrogen treatment of 1-year-old and 2-year-old bamboo could improve the nutrient utilization efficiency of N and P in the leaves of connected ramets, and weakened with the increase of N concentration and treatment time, but both of them could maintain relatively stable N/P and regulate the contents of C, N and P in leaves and their stoichiometric ratios adaptively. Conclusion Nitrogen clonal integration of Ph. violascens has obvious ramet age effect. It is advisable to select 2-year-old bamboo for plant-hole fertilization, which can greatly improve the fertilizer utilization rate and significantly reduce the amount of fertilizer used.
DNA Extraction and COI Gene Amplification of Lymantria dispar Specimens
Yao XU, Hong-bin WANG, Mei WANG, Guo-hong LI
2020, 33(2): 43-53.   doi: 10.13275/j.cnki.lykxyj.2020.02.006
[Abstract](302) [FullText HTML](213) [PDF 1035KB](14)
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Objective The preservation and DNA extraction methods for Lymantria dispar specimens were discussed, and the effects of storage time and preservation methods of L. dispar specimens on amplification of gene sequences were analyzed. Method Genomic DNA of L. dispar specimens (dried adult, formalin-fixed larval and fresh larval specimens) was extracted by SDS method, the magnetic bead method, and the E.Z.N.A.TM Insect DNA extraction kit. Moreover, the concentration and purity of extracted genomic DNA obtained by the three methods were analyzed and compared. The L. dispar specimens collected from 1956 to 1996 were used to analyze and compared the effects of storage time and preservation methods on the amplification success rate of 15 pairs of COI (cytochrome oxidase subunit I) gene primers (the length of the target fragment is 216 bp to 977 bp). Result The results showed that the concentration and purity of extracted genomic DNA by E.Z.N.A.TMInsect DNA extraction kit was the highest compared with the SDS method and the magnetic bead method. And the specimens' DNA was not damaged during the extraction process. The amplification success rate of specimens with longer storage time was reduced. Moreover, the overall amplification success rate of formalin-fixed larval specimens was slightly higher than that of dried adult specimens. Conclusion The E.Z.N.A. TM Insect DNA extraction kit is more suitable for DNA extraction of L. dispar specimens (dried adult, formalin-fixed larval and fresh larval specimens). The amplification of gene sequences is affected by the storage time and preservation method of L. dispar specimens.
Studies on Flowering Biological Characteristics of Pleioblastus pygmaeus
Hua-jun FU, Ting-ting FAN, Meng YANG, Ya-wen WAN, Yu-long DING, Shu-yan LIN
2020, 33(2): 54-60.   doi: 10.13275/j.cnki.lykxyj.2020.02.007
[Abstract](264) [FullText HTML](211) [PDF 829KB](12)
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Objective To study the flowering biological characteristics and pollen morphology of Pleioblastus pygmaeus. Method Sample plots were selected for continuous observation and record among flowering P. pygmaeus. By morphological observation and scanning electron microscopy, the inflorescence and pollen of P. pygmaeus were observed for the purpose of evaluating the population and individual changes of P. pygmaeus at different flowering stages. Result P. pygmaeus belongs to the type of flowering wholly, parts of bamboo culms would died after blossoming. It is possible for the flowering P. pygmaeus to blossom from shoot buds to old culm buds. Flower buds differentiate in late October and flourish from late March to early April. It blooms most in the morning. The flowers are opened from the base to the top in a spikelet. Glumous flowers are monochogamy, 1 glumelle and 1 lemma, 3 lodicules, 3 stamens, 1 pistil, and fruit is caryopsis. Pollen grains are spheroidal, exine is roughness, 1 aperture. Conclusion P. pygmaeus is a kind of dwarf mixed bamboo, tending to decline after flowering, its development is slow at the early stage, and accelerates after the temperature warming up in the next year. Though it has a certain natural rejuvenation ability because it can be reproduced by seeds, but artificial planting with seeds is also necessary.
Thinning and Tending of Natural Secondary Forest of Quercus mongolica Based on Volume Growth Rate
Feng QUAN, Chao-fan ZHOU, Guang-shuang DUAN, Xue-fan HU, Hui-ru ZHANG, Xiang-dong LEI
2020, 33(2): 61-68.   doi: 10.13275/j.cnki.lykxyj.2020.02.008
[Abstract](283) [FullText HTML](113) [PDF 726KB](20)
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Objective To discuss the suitable thinning scheme for different age groups of Quercus mongolica, and provide some reference for thinning and tending of natural secondary forest of Q. mongolica in Jilin Province. Method Based on the data of the 5 forest resources inventories in Jilin Province, the young, middle-aged and near-mature forests of Q. mongolica were screened and five grades of volume thinning intensity were set: intensity 1 (0~10%), intensity 2 (10%~20%), intensity 3 (20%~30%), intensity 4 (30%~40%) and intensity 5 (40%~50%). By comparing the non-thinning stands, this paper studied the change rule of volume growth rate of different age groups at different thinning intensities in each observation intervals (5, 10, 15 and 20 years) after thinning. Result (1) The variations of volume growth rate changed with thinning intensity in different periods after cutting are as follows. For young forests the growth rate was flat at first, then rose rapidly, and declined rapidly in the end; For middle-aged forests, the growth rate rose slowly at first, and then declined rapidly; For near-mature forests that 5 and 10 years after felling, the growth rate rose rapidly and was flat in the end; For the near-mature forests that 15 and 20 years after felling, the growth rate rose rapidly at first, then declined rapidly and declined slowly in the end. (2) The ranges of thinning intensity of the peak value in each period after cutting were 20% to 35% for young forest, 15% to 35% for middle-aged forest that 5 and 10 years after cutting, 20% to 40% for middle-aged forest that 15 and 20 years after cutting, 15% to 25% for near-mature forest that 5 and 10 years after cutting. 15% to 35% for near-mature forest that 15 and 20 years after cutting. (3) The mean volume growth rate of young forest with thinning intensity 3 and 4, middle-aged forest with thinning intensity 2, 3, 4, near-mature forest with thinning intensity 2 and 3. (4) The difference in volume growth rate between various thinning intensities and no thinning was getting smaller over time. Conclusion The suitable thinning intensities for natural secondary forest of Q. mongolica in Jilin Province are medium-high intensity (30%~40%) for young forest, medium or medium-high intensity (20%~40%) for middle-aged forest, and medium intensity (20%~30%) for near-mature forest.
The Factors Affecting Carbon Storage in Degraded Forest Ecosystem: A Case Study from Daxing'anling Areas of Inner Mongolia
Xiao HE, Hai-kui LI, Lei CAO, Sheng-lin XU, Xiao-tong LIU
2020, 33(2): 69-76.   doi: 10.13275/j.cnki.lykxyj.2020.02.009
[Abstract](585) [FullText HTML](703) [PDF 639KB](26)
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Objective To determine the factors affecting carbon storage in degraded forest ecosystem and provide references for the restoration of carbon sink function. Method Based on the survey data, and considering the stand factor and abiotic environmental factors (terrain factors and climatic factors), the variance analysis of stepwise regression and structural equation model method (SEM) were used to study the factors of carbon sequestration. Result Two methods obtained basically consistent results: R2 from the variance analysis method was 0.890. The average diameter at breast height (DBH) and tree number density among the stand characteristics, the monthly mean maximum temperature and monthly mean minimum temperature among the abiotic environment and the disturbance type had significant effects on the stand carbon storage. The stand carbon of R2 derived from SEM was 0.757. For the stand characteristics, the average DBH and tree density affected the stand carbon the most. Tree density had directly positive and indirectly negative effects while among abiotic environment factors, the monthly mean maximum temperature had the indirectly negative effects on the carbon storage of stand. The impact of each variable on stand carbon followed the order of average DBH (0.94) > the monthly mean maximum high temperature (0.52) > tree density (0.12). Conclusion Combining the results of the two methods, it is concluded that in the degraded forest ecosystems, both stand factors and climatic factors have significant impact on stand carbon storage while terrain factor has no significant impact. The results of this study could provide references for studying the carbon storage in Daxing’anling forest area.
Preliminary Study on the Function of Cinnamoyl-CoA Reductase Gene PeCCR of Moso Bamboo (Phyllostachys edulis)
Hao XU, Ke-bin YANG, Cheng-lei ZHU, Ying LI, Zhi-min GAO
2020, 33(2): 77-84.   doi: 10.13275/j.cnki.lykxyj.2020.02.010
[Abstract](321) [FullText HTML](232) [PDF 1126KB](19)
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Objective To reveal the effect of cinnamoyl-CoA reductase (CCR) gene expression on bamboo lignin, the expression of PeCCR in moso bamboo (Phyllostachys edulis) was analyzed, and the gene function of PeCCR was studied, which provided a reference for bamboo breeding using genetic engineering with CCR gene. Method Real-time quantitative PCR (qRT-PCR) was used to analyze the expression of PeCCR in different tissues and the shoots with different heights of moso bamboo. The coding region of PeCCR was cloned by RT-PCR and its overexpression vector was constructed. PeCCR was transformed into Arabidopsis thaliana by floral dip method, and the content of lignin in transgenic plants was determined by bromoacetyl method. Result The results of qRT-PCR showed that the expression level of PeCCR was the highest in the roots of moso bamboo seedling, followed by the shoots, and the lowest in the unexpanded leaves. Under the natural environment, with the increase of bamboo shoot height, the degree of lignification increased, and the gene expression of PeCCR showed an upward trend, reaching the highest in 6.7 m bamboo shoots. The coding region of PeCCR was 1 026 bp, encoding a 341 aa protein with a conserved domain of "KNWYCYGK" unique to the CCR family. Phenotypic analysis showed that compared with the wild type, the leaves of PeCCR transgenic Arabidopsis plants became larger obviously, and the bolting time was 3-4 days earlier. The histochemical staining of stem transverse sections showed that the staining area of xylem and inter-fiber tissue in transgenic plants were larger than that of wild type. The lignin measurement demonstrated that the lignin content of two transgenic lines overexpressing PeCCR1 was significantly higher than that of wild type (123.1% and 116.7% of the wild type control, respectively). Conclusion PeCCR was differently expressed in different tissues of moso bamboo, and it was upregulated in bamboo shoots with increasing height. Overexpression of PeCCR promoted the growth and development of transgenic Arabidopsis plants with increased lignin content.
Cloning and Characterization of Brassinosteriod Biosynthesis-related Gene DET2 in Poplar
Ze-hua LI, Juan DU, Xue-jiao HE, Shu-tang ZHAO, Ying-li LIU, Meng-zhu LU
2020, 33(2): 85-92.   doi: 10.13275/j.cnki.lykxyj.2020.02.011
[Abstract](284) [FullText HTML](261) [PDF 1185KB](27)
Abstract:
Objective Brassinosteriods(BRs)as essential plant hormones play crucial roles in plant growth and development. DET2, a 5α-reductase, is considered to catalyze a major rate-limiting in BRs biosynthesis. Study on PagDET2 gene is useful to understand the role of BRs in woody plants. Method In this study, PagDET2, a homologus gene of Arabidopsis AtDET2, was isolated from Poplar 84K (Populus alba × P. glandulosa, ‘84K’). Bioinformatic method was used to sequence alignment and analyze the basic physical, chemical characteristics and evolutionary relationship. Tissue expression patterns in poplar was analyzed by RT-PCR. The over-expression vector was constructed and transformed into poplar 84K. PagDET2-OE and wild-type plants were used to measure the content of BRs in vivo and analyze the role of DET2 in plant growth and stresses resistance. Result The CDS of PagDET2 gene was 774bp, encoding a 257 amino acid protein. The protein sequence of PagDET2 protein were conserved among Populus trichocarpa, Arabidopsis thaliana, rice, cotton, soybean and tomato. Tissue specific expression analysis showed that PagDET2 was detected in root, leaf tissue and stem tissue. Stem tissue of internode 1 to 3 showed the highest expression level among these tissues. The BRs content of poplar was measured by ELISA. DET2 could significantly increase the endogenous BRs content of poplar. Overexpression of DET2 gene can promote plant growth and be more sensitive to salt stress. Conclusion Overexpression of PagDET2 can significantly increase the endogenous BRs content and increase plant growth. PagDET2 may also be involved in the wood formation and internode elongation.
Variation in Morphological Characters and Nutrient Contents of Quercus fabri Fruits from Different Provenances
Shi-fa XIONG, Li-wen WU, Yi-cun CHEN, Ming GAO, Xin-hua ZHOU, Yang-dong WANG
2020, 33(2): 93-102.   doi: 10.13275/j.cnki.lykxyj.2020.02.012
[Abstract](290) [FullText HTML](211) [PDF 744KB](20)
Abstract:
Objective To provide references for breeding and germplasm resources development of Quercus fabri by analyzing the variations of fruit morphology and nutrient composition of Q. fabri from different provenances. Method The fruits of 368 individual plants from 14 Q. fabri provenances were collected, and 4 morphological characteristics and 6 nutrient content indicators of each fruit were measured, then, the statistical analysis, principal component analysis and cluster analysis were conducted. Result (1) There were extremely significant differences in the 4 morphological characteristics of Q. fabri among and within these provenances, and there were significant differences in 6 nutrient contents among provenances. The mean value of Shannon-Wiener (H) of fruit morphology characteristics was 1.839, in which the values of fruit width, fruit length, length-width ratio and 100-grain mass were 1.886, 1.837, 1.832, and 1.801. (2) The results of correlation analysis showed that most of the 10 characteristic parameters had significant or extremely significant correlations, however, only a few of them had significant correlations with geographical and climatic factors. (3) According to the results of Q-type cluster analysis of 10 characteristic parameters, the 14 Q. fabri provenances could be divided into 3 groups. Conclusion There are extremely significant differences in morphological characteristics of Q. fabri fruit within or among provenances, and the difference is greater within provenance than among provenances. There are extremely significant differences in nutrient content among provenances. It shows abundant genetic variations. There is a certain correlation between fruit characteristic parameters and geographic climate factors. Based on the results of cluster analysis and nutrient content analysis, it is proved that the fruit quality of the provenance from Suichang of Zhejiang Province is the best.
Study on the Factors Affecting Adventitious Shoots Proliferation and in vitro Regeneration of Quercus virginiana
Hai-jing SUN, Xiang SHI, Yi-tai CHEN, Shu-feng WANG, Qin-di XU
2020, 33(2): 103-111.   doi: 10.13275/j.cnki.lykxyj.2020.02.013
[Abstract](332) [FullText HTML](347) [PDF 1254KB](8)
Abstract:
Objective To study the factors that affect the adventitious buds development and rooting process, so as to develop an efficient in vitro propagation methodological system in live oak (Quercus virginiana). Method Using stem segments as explants, the effects of basal medium, browning control, the origin of explant, and different hormone combinations on cluster buds proliferation and rooting were studied. Result The results showed that the addition of ascorbic acid, sodium thiosulfate, and powdered activated carbon could effectively reduce the browning range of explants compared with the control (p<0.05), among which 3.00 g·L−1 and 5.00 g·L−1 powdered activated carbon were proved to be the most effective. The growth medium screening test showed that more and longer shoots were achieved on the low salt medium with a quarter of Murashige and Skoog (MS) medium or woody plant medium (WPM). And the browning was also alleviated with the low salt medium in this study. The highest proliferation rate (6.6 buds per explant) was achieved on a quarter of MS medium supplemented with 1.20 mg·L−1 6-benzylaminopurine (BA) after 40 days. In vitro regenerated shoots were rooted on a quarter of MS medium supplemented with 0.50 mg·L−1 indole-3-butyric acid (IBA) and 0.50 mg·L−1naphthalene acetic acid (NAA) with the highest rooting rate 53.33% and more lignified roots. The plantlet was transferred to sterile sand after 3-5 days acclimation in greenhouse and 57.78% survival rate was observed finally. Conclusion Based on the repeated experiments during the past three years, it is confirmed that the basal medium composition and the proportion of hormone combination will be the major factors determining the proliferation of adventitious shoots and in vitro regeneration of Q. virginiana. The basal mediums that contain lower concentration of inorganic salt are proved to be favorable not only for the adventitious shoots proliferation, but also for alleviating the browning of explants. It is concluded that the explants from the field are much easier to become brown than that from sterile seedlings.
Dynamics of Groundwater Level and Its Affecting Factors under Different Wetland Vegetation of Lixiahe Plain, Jiangsu Province
Dong-mei HE, Lei WANG, Xin WAN, Qing XU, De-qiang GAO, Hai-jun ZUO
2020, 33(2): 112-117.   doi: 10.13275/j.cnki.lykxyj.2020.02.014
[Abstract](199) [FullText HTML](130) [PDF 961KB](6)
Abstract:
Objective To understand the dynamic characteristics of groundwater level in typical freshwater wetland vegetation in the Lixiahe Plain which is rich in water resources in Jiangsu Province. Method Two groundwater depth monitoring wells were set respectively in two typical freshwater wetland vegetations (Taxodium ascendens coniferous forest and Poplar broadleaved forest) in Lixiahe Plain of Jiangsu province. The monthly and seasonal variation of groundwater level were analyzed based on the long-term observation data of groundwater level. In addition, the temperature and precipitation data of the forests during the hydrological year from September 2016 to August 2017 were also collected. The correlations between the dynamics of groundwater level and the variation of temperature and precipitation were analyzed. Result There were significant differences in the dynamics of groundwater level between the two freshwater wetland vegetations. T. ascendens forest was almost flooded all the year round, and its monthly mean groundwater level was higher than that of Poplar forest. The amplitude of monthly variation of groundwater level in Poplar forest was larger than that of T. ascendens forest, and it showed a seasonal variation. On the seasonal scale, the average groundwater level in T. ascendens forest and Poplar forest in summer was significantly lower than that in other seasons. The effects of precipitation on the variation of groundwater level of the two freshwater wetland vegetations were different. The change of groundwater level in Poplar forest lagged behind the amount of precipitation about one month, while this lag did not appear in T. ascendens forest. The average monthly groundwater level was negatively correlated with the mean monthly temperature in both vegetation types. Conclusion It is concluded that there are differences in the dynamics of groundwater level and the influencing factors between the two types of freshwater wetland vegetation. In Lixiahe Plain with abundant water resources, temperature rather than the precipitation is the key factor affecting the change of groundwater level in the freshwater wetland vegetation.
Correlation Analysis of Different Hazard Periods of Tomicus spp. Based on Hyperspectral Features and Photosynthetic Parameters
Meng-ying LIU, Lei SHI, Yun-qiang MA, Zhong-he ZHANG, Xue-lian LIU, Jun YAO, Ting DU
2020, 33(2): 118-127.   doi: 10.13275/j.cnki.lykxyj.2020.02.015
[Abstract](313) [FullText HTML](268) [PDF 1065KB](10)
Abstract:
Objective To establish monitoring models and photosynthetic parameter simulation equations by measuring the hyperspectral data and photosynthetic data of Pinus yunnanensis, so as to effectively and quickly predict the damage period of Tomicus spp. and diagnose the health status of P. yunnanensis, and provide a reference for large-area application of hyperspectral remote sensing technology to achieve forest pest monitoring. Method The damage caused by Tomicus spp. was investigated and the hyperspectral and photosynthetic data were obtained. The parameters which were significantly correlated with the damage period of Tomicus spp. were selected to establish monitoring models. The correlation between photosynthetic parameters and hyperspectral characteristic parameters was analyzed and the correlation equations of photosynthetic parameters were established. Result As the hazard period prolonged, the spectral reflectance gradually decreases in the range of 740-1036 nm. "Red edge" and "blue shift" appeared in the first-order differential curve of the spectrum at 660-740 nm, and the peak value gradually decreased. The multivariate linear regression model which was established based on spectral characteristics showed the best fitting effect. The damage period of Tomicus spp. was closely correlated with Photo, and its cubic function model fitted well. The correlation between spectral index and photosynthetic parameters was established to obtain the optimal fitting equation of photosynthetic parameters. Conclusion The models based on spectral index and photosynthetic parameters of P. yunnanensis can effectively monitor the hazard period of Tomicus spp. There is a significant correlation between the spectral index and photosynthetic parameters of P. yunnanensis, and a correlation model can be established to estimate the growth and health status of P. yunnanensis.
Two-dimensional Electrophoresis Profiles for Proteins of Poplar Leaf
Hui-ying SUO, Mi ZHENG, Han LU, Guan-zheng QU, Ying LI
2020, 33(2): 128-137.   doi: 10.13275/j.cnki.lykxyj.2020.02.016
[Abstract](181) [FullText HTML](89) [PDF 2338KB](8)
Abstract:
Objective To explore a two-dimensional electrophoresis (2-DE) system suitable for proteomics research of poplar leaf and to establish 2-DE profiles for proteins of poplar leaf. Method 2-DE was used to conduct the optimization of 2D lysis buffer, the purification of protein, the immobilized pH gradient (IPG), sample loading amount, isoelectric focusing (IEF) time and the equilibration time of the 2-DE system of Populus simonii×P. nigra leaves. 2-DE experiments for proteins of P. simonii×P. nigra, P. ussuriensis and P. alba×P. glandulosa leaves were carried out using optimized 2-DE system. Result The solubility of proteins of P. simonii×P. nigra leaves could be significantly improved by using 2D lysis buffer II. There were 326 proteins detected by using 2D lysis buffer II, which were 209 more than that detected by using 2D lysis buffer I. The proteins of P. simonii×P. nigra leaves were extracted by plant protein lysis buffer and purified by 2D clean-up kit. The background of the 2-DE profile was clear and the protein separation effect was better. The proteins of P. simonii×P. nigra leaves was mainly distributed in the range of pH 4~7, and 393 proteins could be obtained with good separation using IPG strips of 24 cm in length with the pH ranges from 4 to 7. The proteins detected increased from 326 to 454 when the proteins loading amount was up to 1 mg. With the condition of 6 h IEF time of 10 000 V and 40 minutes of Sodium Dodecyl Sulfate equilibration time, the background of 2-DE profile was more clear, more proteins were detected and the resolution was higher. There were 531, 828 and 525 proteins, which were detected from P. simonii×P. nigra, P. ussuriensis and P. alba×P. glandulosa leaves separately by using optimized 2-DE system. The separation of the protein was clear and the resolution was high. Conclusion This study optimized the key steps of 2-DE system of protein of P. simonii×P. nigra leaves and establish 2-DE profiles for proteins of P. simonii×P. nigra, P. ussuriensis and P. alba×P. glandulosa leaves by optimized 2-DE system. This research reveals that it is possible to improve the resolution and repeatability of 2-DE system of protein of P. simonii×P. nigra leaves by the optimized system. Using the optimized 2-DE system, the authors have successfully established 2-DE profiles for proteins of P. simonii×P. nigra, P. ussuriensis and P. alba×P. glandulosa leaves. This system is suitable for the proteomics analysis of P. simonii×P. nigra, P. ussuriensis and P. alba×P. glandulosa leaves. This result is a reference for proteomics analysis of poplar leaf.
Response of Antioxidant System of Salix viminalis under Phenanthrene Stress
Xia LI, Xiao-dong MA, Jun-zhu ZOU, Xiao-xing ZHOU, Zhen-yuan SUN, Lei HAN
2020, 33(2): 138-144.   doi: 10.13275/j.cnki.lykxyj.2020.02.017
[Abstract](146) [FullText HTML](98) [PDF 944KB](9)
Abstract:
Objective In order to explore the defense mechanism of the antioxidant system of Salix viminalis , the dynamic changes of reactive oxygen and antioxidant substances under phenanthrene stress were observed which can provide new evidence for improving the resistance of plants to polycyclic aromatic hydrocarbon (PAHs) stress and enhancing the phytoremediation potential. Method The cutting seedlings of S. viminalis were selected as experimental materials, and a 16-day hydroponic experiment with 0 and 1.0 mg·L-1 phenanthrene concentrations were conducted to study the dynamic changes of reactive oxygen, antioxidant enzymes, antioxidants and malondialdehyde (MDA) contents. Result The Results showed that: (1) Under phenanthrene treatment, the superoxide anion radical (O2·-) production and H2O2 content increased rapidly, then MDA content rose. Catalase (CAT) activity significantly increased on the 4th day, and the superoxide dismutase (SOD) and peroxidase (POD) activity increased on the 8th day. On the 16th day, no significant difference was found in H2O2 content between the samples treated with phenanthrene and that of the control. Meanwhile, the increment of O2·- and MDA also slowed down. (2) The contents of reduced glutathione (GSH) and glutathione reductase rose rapidly on the 4th day under phenanthrene treatment, and the activity of glutathione-s-transferase (GST) increased slowly. (3) Under phenanthrene treatment, the content of ascorbic acid was initially lower than the control group, but higher on the 16th day as the treatment time continued. Conclusion Under phenanthrene stress, O2·- is the main reactive oxygen causing cell membrane lipid peroxidation. SOD activity is always higher than that of the control group, but not enough to eliminate the increased O2·-. The increase of CAT and POD could eliminate the excessive H2O2. GSH is an effective antioxidant to resist the stress of phenanthrene and participates in the detoxification of phenanthrene through the catalysis of GST.
Selective Behavioral Response of Carpomya vesuviana Costa to Volatile Substances of Jujube
Meng LIANG, Aziz ABDUWAHAP, Sattar ADIL
2020, 33(2): 145-153.   doi: 10.13275/j.cnki.lykxyj.2020.02.018
[Abstract](291) [FullText HTML](218) [PDF 602KB](8)
Abstract:
Objective To study the volatile substances of jujube trees attractive to Carpomya vesuviana Costa. Method The experiment was carried out in July 2016. The choice behavior of adult C. vesuviana to 6 kinds of volatile substance from jujube fruit was measured. On the basis of laboratory test, field validation test was carried out. Result The result of selection behavior experiment in laboratory showed that the adult C. vesuviana had significant positive tropism for the 6 kinds of volatile substance. The selectivity of female to ethyl palmitate, ethyl laurate and myristic acid was stronger, which was higher than 30%. The selectivity and selectivity coefficient of different concentration gradients (stock, 10% solution, 1% solution) were observed. It was showed that the selection rate and selection coefficient decreased with the decrease of concentration. The selectivity to the stock solution was 2.5 times and 5 times that of 10% solution and 1% solution. In field trials, it was found that the effect of myristic acid on the female of C. vesuviana was stronger, the maximum number of 2 d traps was 15/head, and the average number of traps was 11.4/head, followed by ethyl myristate. Ethyl oleate and ethyl linoleate had stronger attraction effect on the male C. vesuviana. As far as the attracting effect is concerned, there were significant differences between male and female C. vesuviana to ethyl laurate and ethyl myristate, while there was no significant difference among other volatiles. Conclusion Among the volatile substances of jujube fruit, ethyl palmitate, ethyl laurate and myristic acid are closely related to the preference of C. vesuviana. In field trials, the selectivity of adults C. vesuviana Costa to myristic acid is higher, which can provide a theoretical basis for the research and development of attractant.
Study on Nutritive Substances and Medicinal Components of Sorbus pohuashanensis
Man-man XU, Xue-dan YU, Yong-qi ZHENG, Tao ZHANG, Xin-he XIA, Qi-di FU, Chuan-hong ZHANG
2020, 33(2): 154-160.   doi: 10.13275/j.cnki.lykxyj.2020.02.019
[Abstract](356) [FullText HTML](369) [PDF 602KB](17)
Abstract:
Objective Taking Sorbus pohuashanensis as object to study the discrepancy among different parts of S. pohuashanensis and the impact of different habitat conditions on the content of the substances. Method The nutritive and medicinal substances of different parts of S. pohuashanensis (twigs, young leaves, mature leaves, fresh fruits and dried fruits) were extracted by organic solvent and the compositions were determined by liquid chromatography. Result Different parts of S. pohuashanensis are rich in nutritive and medicinal substances. The contents of vitamin C, carotenoids, flavonoids in mature leaves and protein, soluble sugar, total phenols, anthocyanin, tannin in dried fruits are higher than that in other parts. Compared with S. discolor, the contents of protein, anthocyanin, flavonoids and total phenols in S. pohuashanensis are higher. The contents of anthocyanin and carotenoids in fruits of S. pohuashanensis are about 3 times and 5 times of that in S. discolor respectively. The contents of flavonoids and total phenols in mature leaves of S. pohuashanensis are about 2 times of that in S. discolor. The fresh fruit of S. pohuashanensis is rich in flavonoids and phenolic compositions, of which the contents of quercetin and epicatechin are the highest, and about 1.5 times and 3 times of that in S. discolor respectively. The contents of various substances in fresh fruits of S. pohuashanensis are different in different habitats. Conclusion S. pohuashanensis is of higher nutritive and medicinal components than S. discolor, and the flavonoid and phenols contents in leaves and fruits is worth to be further exploited and utilized.
Mound Building Effects of Red Fire Ants (Solenopsis invicta Buren) on the Concentrations of Soil Nitrogen, Phosphorus and Potassium across Different Habitats
Jian-xiu YANG, Nian-nian ZHANG, Di YAN, Qiao LI, Qing WANG, Zhi-xing LU, You-qing CHEN
2020, 33(2): 161-167.   doi: 10.13275/j.cnki.lykxyj.2020.02.020
[Abstract](167) [FullText HTML](102) [PDF 611KB](9)
Abstract:
Objective This study aims at revealing the effects of mound building of Solenopsis invicta on soil nitrogen, phosphorus and potassium concentrations across three habitats. Method Three habitats (forestland, wasteland, and grassland) were selected as research sites in Kunming and Mouding, Yunnan Province in November 2017 and March 2018. The characteristics of nitrogen, phosphorus and potassium concentrations in active and abandoned nests of S. invicta were compared. Result Ant colonization significantly increased the soil nitrogen, phosphorus and potassium concentrations in all the three habitats (P<0.01). The phosphorus and potassium concentrations were significantly higher in abandoned nests than in the active nests in forestland (P<0.05 or 0.01), while that in grassland were significantly higher than in abandoned nests (P<0.05 or 0.01). However, there was no significant difference in nitrogen concentration between active and abandoned nests in forestland and grassland, and no significant difference was found in nitrogen, phosphorus and potassium concentrations among different soil layers. Ant colonization significantly increased the concentrations of nitrogen, phosphorus and potassium in loam soils and sandy soils. The rising level of phosphorus and potassium contents was extremely higher in loam soils than in sandy soils (P<0.01). Conclusion The colonization of S. invicta can significantly increase soil nitrogen, phosphorus and potassium concentrations in the three habitats. This effects will be still significant after active nests are abandoned, and the increase in loam soils will be higher than that in sandy soils.
2020, 33(2): 181-181.  
[Abstract](141) [FullText HTML](124) [PDF 164KB](3)
Abstract:
Review
Research on the Pheromone Compounds of Cerambycidae Insects
Ying ZHU, Zhi-jia HUANG, Lai-jiao LAN, Jin-kun ZHANG, Qing-yuan GAO, Lin WANG, Yong-hu LI, Xiu-jun WEN, Tao MA
2020, 33(2): 168-180.   doi: 10.13275/j.cnki.lykxyj.2020.02.021
[Abstract](130) [FullText HTML](103) [PDF 1420KB](18)
Abstract:
The identification and application of insect pheromone compounds play an important role in integrated pest management (IPM). Pheromone compounds are now identified from more than 100 species belonging to Cerambycidae. The pheromones identified to date from species in the subfamilies Cerambycinae, Spondylidinae, and Lamiinae are all male aggregation pheromones, whereas all known examples for species in the subfamilies Prioninae and Lepturinae are female sex pheromones. These pheromone components have a high overlapping ratio among the subfamilies, especially among the genera and the species. The same components can play a role in many species of Cerambycidae. The application potential and broad prospects of pheromone compounds regulating insect behavioral activities in pest management have made the research of pheromone compounds more important. To make a systematic analysis on the known pheromone components of Cerambycidae can provide evidence for the further identification of the pheromone components of other Cerambycidae species, and promote the identification and application of pheromone components in Cerambycidae.
Effect of Thinning on Growth and Timber Outturn in Cunninghamia lanceolata Plantation
XU Jin-liang, MAO Yu-ming, ZHENG Cheng-zhong, FAN Rong-de, ZHOU Shi-shui, CHEN Yong-hui, CHENG Xiang-rong, YU Mu-kui
2014, 27(1): 99-107.  
[Abstract](1382) [PDF 992KB](1409)
Advances in Tissue Culture Techniques of Trees and the Problems Existed
HUANG Lie-jian, WANG Hong
2016, 29(3): 464-471.  
[Abstract](999) [PDF 914KB](1582)
Comparison on Osmotica Accumulation of Different Salt-tolerant Plants under Salt Stress
CHENG Tie-long, LI Huan-yong, WU Hai-wen, LIU Zheng-xiang, WU xiang, YANG Sheng, ZHANG Hua-xin, YANG Xiu-yan
2015, 28(6): 826-832.  
[Abstract](1130) [PDF 1605KB](1095)
Growth and Structure Differentiation of Phoebe bournei Plantation with Different Sites and Modes of Afforestation
CHU Xiu-li, LIU Qing-hua, FAN Hui-hua, WANG Sheng-hua, CHEN Liu-ying, ZHOU Zhi-chun
2014, 27(4): 445-453.  
[Abstract](1380) [PDF 1443KB](1353)
Studies on Seedling Photosynthetic Characteristics of Five Tree Species under Drought Stress
HAN Bo, LI Zhi-yong, GUO Hao, ZHANG Jun-pei
2014, 27(1): 92-98.  
[Abstract](1245) [PDF 1078KB](1248)
Quantitative Analysis of Forest Spatial Structure and Optimal Species Composition for the Main Forest Types in Daxing'anling, Northeast China
DONG Ling-bo, LIU Zhao-gang, LI Feng-ri, JIANG Lei
2014, 27(6): 734-740.  
[Abstract](1415) [PDF 1059KB](1154)
Hydrological Effects of Litters and Soil under Different Forests in Jiulongshan of Beijing
WU Di, XIN Xue-bing, ZHAO Ming-yang, PEI Shun-xiang, KONG Ying
2014, 27(3): 417-422.  
[Abstract](1157) [PDF 1225KB](1068)
Research Progress of Structure-based Forest Management
Gang-ying HUI, Yan-bo HU, Zhong-hua ZHAO
2018, 31(1): 85-93.   doi: 10.13275/j.cnki.lykxyj.2018.01.011
[Abstract](1781) [FullText HTML](302) [PDF 2383KB](710)
Effect of Substrate Ratio and Slow-release Fertilizer Dose on the Growth of Containerized Cyclobalanopsis gilva Seedlings
WU Xiao-lin, ZHANG Dong-bei, CHU Xiu-li, WANG Xiu-hua, ZHOU Zhi-chun
2014, 27(6): 794-800.  
[Abstract](1317) [PDF 921KB](1118)
Establishment of Compatible Tree Volume Equation Systems of Chinese Fir
ZENG Wei-sheng
2014, 27(1): 6-10.  
[Abstract](1424) [PDF 912KB](1359)

Journal Information

Publication name:林业科学研究 Forest Research

Editor:ZHANG Shou-gong

Sponsors by:Chinese Academy of Forestry Sciences

Address:Chinese Academy of Forestry after Beijing Wanshou Mountain

Telephone:010-62889680;62889702

E-mail:lykxyj@caf.ac.cn

WebSite:http://www.lykxyj.com

ISSN 1001-1498

CN 11-1221/S

Postal code:80-717

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