Abstract: This paper reports a method for mass micropropagation of jojoba. Plant growth regulators, basal media and sugar level were examined to determine their effects on shoot growth, shoot multiplication and rooting in vitro. The results showed that the nodel explants from 1 year old seedlings grown in spring and 6 years old, high yield, individual trees were cultured on MS medium supplemented with ZT 1~3mg/L or BA 1~3 mg/L and NAA 0.01~0.1 mg/L. Shoots were obtained from all the axillary buds after 30~40 days of culture. Shoots measuring about 4~6cm can be excised and rooted in 1/2 MS medium added with NAA following one week in dark for rooting. About 93% rooting was achieved after one month. The plantlets were placed into moist chamber with sand-soil medium successfully. No major differences on in vitro propagation between young seedlings and adult plants of jojoba were found. It appears that the method may not be limited by genotype.