Abstract: The results of the study on environmental condition for the growth of 3 species of Eucalyptus mycorrhizal fungi,Scleroderma 9215,Pisolithus 9216, Hebelima E4070 showed that isolate 9216 had the widest suitable temperature range for growth,from 10 to 38 C,and isolate E4070 had the narrowest suitable temperature range for growth,from 15 to 30 C ;isolate 9215 had the widest suitable pH range,from 3 to 7, and isolate E4070 had the narrowest suitable pH range,from 5 to 7.Except that isolate 9216 could not use organic N from beef extract and yeast extract,the others could use 8 kinds of N resource at different levels.and isolate 9215 could use organic N better than other N resources, isolate 9216 could use ammonia N better than nitrate N as to the use of C,except that fructose could not be used well by isolate 9215,the difference of C resources to isolate 9215 was not obvious; maltose and glucose were quite good for isolate 9216, and there was no difference between 6 kinds of carbon resource to isolate E4070.