Abstract: Fruit bearing number and seed yield of Chinese chestnut increased over 20 percent with spraying treatment of TDS plant growth regulator solution (0.25, 0.5, 0.75, 1.0 percent) after revealing of male inflorescence. Besides spraying treatment, TDS solution can be used to inject into trunk or pour on roots of Chinese chestnut. There are obvious effect of increasing fruit bearing number and seed yield when mixture solution (TDS and isopentenyl adenine, 500 mg/kg or TDS and paclobutrazol 500 mg/kg) is used. It is conducive to TDS’s regulation to apply fertilizer in winter or in spring. The results showed that fruit bearing number and seed yield of Chinese chestnut could be raised every year, when TDS regulator was used every year.