Abstract: Suillus luteus (L.ex Fr.) is a superior mycorrhiza fungi being symbiosis with Pinus massoniana in the subtropical area of China. The fungi is widely distributed and has been effectively applied to the pine. In order to examine the liquid cultural conditions of the S. luteus, the temperature,pH,carbon sources,nitrogen sources and C/N for the growth and the propagation of the fungi were tested in the study. The results showed that for the growth and propagation of the hyphae of the strain S.l, the suitable temperature range was 20～30 ℃, the optimum 25 ℃;the suitable pH was 3～8,the optimum 4～4.5. All the carbon sources tested can be absorbed (glucose,fructose,saccharose,amylomaltose,ethanol,mannitol, soluble starch,dextrin) but the optimum was soluble starch;all the nitrogen sources [(NH4)2SO4,(NH4)2HPO4,NH4NO3,KNO3,Urea,tartaric acid,aminoacetic acid,glutamic acid],except inorganic nitrogen (KNO3),can be absorbed but the optimum was glutamic acid. A wider range of C/N was effective but the optimum was 20:1.