Abstract: Four strains, 2# (Bacillus polymyxa), 12#,14# (B.licheniformis), isolated from Phyllostachys heterocycla var.pubescens rhizosphere, 7#(The species name have not been determined finally),isolated from P.meyeri rhizosphere were cultured and prepared into liquid inoculums and inoculated back to moso bamboo (Phyllostachys heterocyclavar.pubescens) seedlings.Potted culture (sand culture) under artificial and sterile conditions was then conducted.The results showed that all of four strains were,more or less, able to raise the survival rate and promote the seedling growth in various aspects.Especially for root fresh weight were increased by 44.1%～76.9%,reaching significant level (14#) and remarkable significant level (2#,12#,7#) respectively.The nitrogen content of seedlings was also increased considerably,increase rate reach to 22%～38%.Meanwhile,statistical analysis results showed, under the condition of no indigenous bacteria, inoculating stage will also influence the growth of seedling,when inoculated on seedling stage the seedlings’growth and total nitrogen content was better and higher than that inoculated on seed germinating stage,the diversity reach to remarkable significant level.