Abstract: Aimed at drafting the germplasm resources conservation strategy of Yunnan forest tree species, some suggestions are made on the basis of the characteristics of the physiography and the flora of forest tree species in Yunnan. They are:(1) The researches are made on the 3 conservation methods of germplasm resources and the sampling strategy based on molecular markers; (2) Conservation in situ and ex situ related to the climatic zones and regions must be carried out with particular emphasis, and 9 centers of conservation ex situ are set up by stages; (3) Forests of conservation ex situ can be planted in the areas of major forestry projects, and some excellent germplasms chosen for the projects; (4) A special conversation bank in low temperature is built for forest tree species under the project of Chinese-Biovalley.