Abstract: Efficient genetic transformation system of five carnation (Dianthus caryophyllus) cultivars has been established and optimized by using leaf explant. The results show that 2 days preculture can increase the transformation frequency obviously. There are differences between carnation cultivars in transformation. 20μmol AgNO3in selection medium inhibits the adventitious shoot inducing. 72.1% transformed plants rooted on rooting medium containing 25 mg·L-1 kanamycin and 55% plants show GUS activity according to histochemical assay. PCR screening indicates that 32.2% of the samples are positive. Southern blot analysis demonstrates that foreign genes are integrated into plant genome.