藏东南云南红豆杉的药用成分含量研究
Contents of Anti-cancer Active Component in Plant of Taxus yunnanensisfrom Southeast Tibet
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摘要: 在藏东南地区林芝县、波密县和察隅县系统采集样品的基础上,通过高效液相色谱(HPLC)测定了野生云南红豆杉(Taxus yunnanensis)树皮、3年生小枝和当年生叶中紫杉醇、巴卡亭Ⅲ、10-去乙酰巴卡亭Ⅲ、三尖杉宁碱、10-去乙酰-7-表紫杉醇、7-表紫杉醇的含量。研究表明:紫杉醇与7-表紫杉醇,巴卡亭Ⅲ与7-表紫杉醇正相关水平极显著 (P<0.001),相关系数分别为0.852和0.827。紫杉醇与巴卡亭Ⅲ呈显著(P<0.05)正相关,相关系数为0.716。紫杉醇含量与7-表紫杉醇含量,巴卡亭Ⅲ含量与7-表紫杉醇含量之间存在线性回归关系,回归系数分别为0.852和 0.827。紫杉醇含量在3年生小枝与当年生叶中的差异达显著水平(P<0.05),而在树皮与3年生小枝、当年生叶中的差异却不显著,树皮、3年生小枝和当年生叶中其它成分含量的差异均未达显著水平。Abstract: The samples of Taxus yunnanensis from southeast Tibet were collected. The anti-cancer component contents in plant of Taxus yunnanensis such as taxol, baccatin Ⅲ, 10-deacetylbaccatin Ⅲ, cephalomannine, 10-deacety-7-epi-taxol and 7-epi-taxol were analyzed by HPLC. The result indicated that the correlation coefficients between of taxol and 7-epi-taxol, 7-epi-taxol and baccatin Ⅲ were 0. 852 and 0. 827. Their positive correlation was markedly in the 0. 001 levels. The correlation coefficient between taxol and baccatin Ⅲ was 0. 716. Their positive correlation was markedly in the 0. 05 levels. The contents of taxol had a significantly correlation with 7-epi-taxol. The contents of 7-epi-taxol had a significantly correlation with baccatin Ⅲ. The regression coefficients between taxol and 7-epi-taxol, 7-epi-taxol and baccatin Ⅲ were 0. 852 and 0. 827. The contents of taxol in 3 years old shoots and 1 year old needles were significantly different at 0. 05 levels. The contents of taxol in barks and 1 year old needles, 3 years old shoots and 1 year old needles were not significantly different at 0. 05 levels.
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Key words:
- Tibet
- / Taxus yunnanensis
- / baccatin
- / cephalomannine
- / epi-taxol
- / contents
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