高CO2 和纯N2 冲击处理对板栗货架期萌芽及激素含量变化的影响
Effect of High CO2 and N2 Shock Treatment on the Sprouting and Hormone Content of Chinese Chestnut(Castanea mollissima Blume) during Shelf-life
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摘要: 以‘燕昌’板栗为试材,研究了50%CO2和纯N2冲击处理5 d和10 d对经过0℃7个月冷藏板栗的货架期呼吸强度、发芽率和胚芽内几种激素(ABA、GA3、IAA、ZR)含量变化的影响。结果表明:(1)纯N2冲击处理对板栗货架期呼吸强度有明显的抑制作用,但50%CO2冲击处理与对照差异不明显;(2)50%CO2冲击处理可明显抑制板栗的发芽,货架期结束时,处理5 d和10 d的板栗发芽率分别为2.07%和3.65%,显著低于对照(18.06%);而纯N2冲击处理则显著促进板栗发芽,货架期结束时,处理5 d和10 d的板栗发芽率分别为45.04%和39.69%;(3)50%CO2冲击处理可明显提高板栗胚芽中ABA含量及ABA/GA3比值,而纯N2冲击处理使板栗胚芽中ABA含量下降,ABA/GA3比值降低;(4)虽然各冲击处理的IAA和ZR含量与发芽率之间没有表现出明显的相关性,但50%CO2冲击处理后ABA/ZR比值较大,纯N2冲击处理10 d明显抑制了ABA/ZR比值,这与各处理影响板栗发芽的结果基本一致。Abstract: In this paper,the effect of 50%CO2 and 100%N2 shock treatment for 5 d and 10 d on the respiration,sprouting and hormone contents(ABA、GA3、IAA、ZR) of Chinese Chestnut(Castanea mollissima Blume,cv.'Yanchang'),which was stored for seven months at 0 ℃,during shelf-life were studied.The research showed that 100%N2 treatments promoted the respiration,while 50%CO2 treatments inhibited the respiration of chestnut in shelf-life.50%CO2 and 100%N2 treatments could affect the sprouting of chestnut remarkably.The rates of sprouting of 50%CO2 treatment for 5 d and 10 d were 2.07% and 3.65% respectively,while those of 100%N2 treatments for 5 d and 10 d were 45.04% and 39.69% respectively,which were much different from the control(18.06%) at the end of the shelf life.50%CO2 treatment increased the ABA content and the ratio of ABA/GA3,100% N2 treatment decreased the ABA content and the ratio of ABA/GA3 in the embryonic bud.Though the contents of IAA and ZR had not showed the close relationship with the spouting,50%CO2 treatment increased the ratio of ABA/ZR,100%N2 treatment decreased the ratio of ABA/ZR,which were basically related to the sprouting rate.
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Key words:
- chestnut
- / shelf-life
- / respiration
- / sprout
- / hormone
- / 50%CO2
- / 100%N2
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[1] 王贵禧,梁丽松,宗亦臣. 贮藏板栗休眠与萌芽的温度调控[J].林业科学, 1999, 35 (3) : 29～33 [2] 彭幼芬. 种子生理学[M]. 湖南:中南工业大学出版社, 1994: 61～84 [3] 梁丽松,王贵禧. 高CO2 和纯N2 冲击处理对板栗货架期品质变化的影响[J]. 林业科学研究, 2005, 18 (3) : 236～240 [4] 郑玉善. 板栗和锥栗种子发育过程中ABA生理效应的研究[J].林业科学, 1998, 34 (4) : 1～7 [5] 余朝霞,黄雪群,方志尚,等. GA对林木种子萌发的调控研究进展[J]. 浙江林业科技, 2003, 23 (1) : 73～76, 80 [6] Tuan-hua David Ho, Aurelio Gomez-Cadenas, Rodolfo Zentella,et al. Crosstalk between gibberellin and abscisic acid in cereal aleurone[J]. J Plant Growth Regulation, 2003, 22: 185～194 [7] Zeevaart J A D, Creelmann R A. Metabolism dissection of the gibberellin / abscisic acid[J]. Annu Rev Plant Physiol PlantMol Biol,1988, 39: 439～473 [8] 刘永庆,罗泽民, Karssen CM,等. 赤霉素和脱落酸对番茄种子发芽的生理调控———文献综述[J]. 园艺学报, 1995, 22 (3) : 267～271 [9] Hocher V, Scotta B, Maldiney R, et al. Changes in levels of abscisic acid and itsβ-D-glucopyranosyl ester during tomato embryo2genesis[J]. Physiol Plant (Supp l) , 1990, 79: A28 [10] HuangW Z, Hsiao A I. Factors affecting dormancy and germinationof Johnson grass, Sorghum halepense (L.) Pers [J]. Weed Science, 1988, 28: 1～12 [11] 王少先,彭克勤,萧浪涛,等. 逆境下ABA 的积累及触发机制[J]. 植物生理学通讯, 2003, 39 (5) : 413～419 [12] Wang X Q, Ullah H, Jones A M, et al. G p rotein regulation of inchannels and abscisic acid signaling in A rabidopsis guard cells[J].Science, 2001, 292: 2070～2072 [13] J iaW S, Zhang J H, Liang J S. Initiation and regulation of waterDeficit-induced abscisic acid accumulation in maize leaves androots: cellular volume and water relations[J]. J Exp Bot, 2001,52 (355) : 295～300 [14] 吴奇,韩继成,何钟佩. 冷藏期间板栗胚内激素含量的变化[J].北京农业大学学报, 1993, 19 (4) : 11～14 [15] Yamaguchi S, SmithMW, Brown R G, et al. Phytochrome regulation and differential exp ression of gibberellin 3beta-hydroxylasegenes in germinating A rabidopsis seeds[J]. Plant Cell, 1998, 10:2115～2126 [16] 徐凯,孙启祥,肖圣元. 板栗种子休眠与萌发的研究[J]. 中国农学通报, 1998, 14 (1) : 24～25, 28 [17] 刘一和,胡小松,李全宏. 板栗长期冷藏技术研究[A]. 食品科学论文集———纪念北京农业大学食品科学系成立十周年[C].北京:北京农业大学出版社, 1994 -
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