引进泰国甜角基因资源多样性的RAPD分析
Ana lysis of the Genetic D iversity of Sweet Tha i Tamr indIntroduced from Tha iland Ba sed on RAPD Markers
-
摘要: 采用RAPD分子标记技术,对引进的10个泰国甜角栽培品种进行分析,研究其遗传变异及遗传关系。筛选出的8个引物共扩增出52条带,其中多态性条带为34条,多态带比率为65. 38%。多态性标记百分率PPL、观测等位基因数Ao、有效等位基因数Ae、Nei’s基因多样性指数H 和Shannon信息指数I在物种水平分别为65. 38%、11653 8、1. 199 9、0. 137 1和0. 227 9,而在栽培品种水平分别为25. 96%、1. 259 6、1. 164 7、0. 094 6和0. 140 3。基因分化系数GST = 0. 163,表明有83. 7%的遗传差异来自栽培品种内。10个栽培品种间的遗传距离变幅在0. 013 1~0.124 4之间,平均为0. 047。UPGMA聚类分析结果显示,以遗传距离0. 02来划分, 10个栽培品种可以分为3组, Zichupoton、Buangka、Barchan、Shampoo、Ziton栽培品种聚为一组, Sritong、Srichompo、Prakaythong、Sritongbao栽培品种构成一组,而Bargeton单独聚为一组。Abstract: Random amp lified polymorphic DNA (RAPD) was emp loyed to study the genetic variation and genetic relationship of 10 sweet Thai tamarind ( Tam arindus ind ica) cultivars introduced from Thailand. The RAPD analysisshowed that 34 of 52 amp lified bands (65. 38% ) were polymorphic within the species, while the mean percentageof polymorphic bandswithin cultivarswas 25. 96%. The number of alleles Ao , effective number of alleles Ae , Nei’sgene diversity H, and Shannon’s information index I for the species were 1. 653 8, 1. 199 9, 0. 137 1 and 012279, respectively, and for the cultivarswere on average 1. 259 6, 1. 164 7, 0. 094 6 and 0. 140 3, respectively. Thelargest p roportion of the total RAPD diversitywas found within, rather than among the cultivars (GST = 0. 163). Thegenetic distance between cultivars ranged from 0. 013 1 to 0. 124 4, and on average was 0. 047. An analysis of 10cultivars using UPGMA showed that the introduced sweet Thai tamarind cultivars could be divided into three group sby 0. 02 genetic distance, the first group included Zichupoton,Buangka,Barchan, Shampoo and Ziton cultivar, thesecond group included Sritong, Srichompo, Prakaythong and Sritongbao cultivar, and the third group included onlyBargeton cultivar. The basic data were p rovided for further development and imp rovement of the cultivars in thisstudy.
-
Key words:
- Tam arindus ind ica
- / RAPD
- / genetic diversity
-
[1] Chindap rasert T S. Tam arindus p lant genetic resources in Thailand[J]. Thai Journal of Agricultural Science, 1996, (1) : 1 - 11 [2] Shankaracharya N N. Tamarind2Chemistry, Technology and Uses2Acritical app raisal [J]. Journal of Food Science & Technology,1998, 35 (3) : 193 - 208 [3] 赵一鹤,杨时宇. 泰国甜角引种栽培试验[J]. 浙江林业科技,2005, 25 (1) : 53 - 55 [4] 赵一鹤,杨时宇,李昆. 泰国甜角不同栽培品种果实营养成分分析[J]. 植物资源与环境学报, 2005, 14 (3) : 57 - 58 [5] Murray M G, Thomp son W F. Rap id isolation of high2molecularweight p lant DNA [J]. Nucleic Acids Research, 1980, 8: 4321- 4325 [6] 王 军,葛玉香,贺普超. RAPD标记在山葡萄种质鉴定中的应用[J]. 植物研究, 2004, 24 (4) : 473 - 476 [7] Yeh F, Yang R C, Boyle T. POPGENE. A User2friendly Sharewarefor Population Genetic Analysis [M]. Molecular and BiotechnologyCenter. University ofAlberta, Edmonton, 1997 [8] Excoffier L. Analysis ofMolecular Variance (AMOVA) Version 1.55 [M]. Genetics and Biometry Laboratory. University of Geneva,Switzerland, 1993 [9] 张富民,葛 颂. 群体遗传学研究中的数据处理方法I. RAPD数据的AMOVA分析[J]. 生物多样性, 2002, 10 (4) : 438 - 444 [10] Nei M. Estimation of average heterozygosity and genetic distancefrom a small number of individuals [J]. Genetics, 1978, 89: 583- 590 [11] 曹家树,申书兴. 园艺植物育种学[M]. 北京:中国农业大学出版社, 2001
计量
- 文章访问数: 3492
- HTML全文浏览量: 190
- PDF下载量: 1437
- 被引次数: 0