Abstract: A kind ofmixed bacterialmanure was p repared, which was composed of four bacterium, namely, B acillusm egatherium var. phospha ticum , B. m ucilaginosus, Azorhizobium sp. and cellulose-decomposing microorganisms.Preservation experiments of the mixed bacterialmanure were carried out under different temperature conditions. Theresults showed thatwhen the temperature was 4, 18 and 28 ℃, the colony counts of each p reserved bacterium increased until 30 days and then almost all of them began to decline, and the total colony counts of the bacterialmanure varied little among 30 days and its growth curve reached a peak and a valley when the temperature was 4 and38 ℃ respectively. This results suggested that the bacterial manure was op timum to be p reserved at 4 ℃ and bekep t for 30 days, and the conditions 4—28 ℃ and 10—90 dayswere the matched ranges for its p reservation. Potting experiments showed that at room temperature and the amount of inoculation was ≤5‰, the growth curves ofAzorhizobium sp. and cellulose2decomposing microorganisms were very similar and both hadn’t a great change, butthat of B. m ega therium var. phosphaticum and B. m ucilaginosus varied significantlywith the increasing amount of inoculation. This suggested that at room temperature, the total colony counts of the bacterial manure would increasewhen the amount of inoculation was ≤5‰ and reached the highest value 90 days later after soil inoculation.