涝渍胁迫对转多基因库安托杨生长及生理性状的影响
Effects of Waterlogging Stress on Growth and Physiological Characters in Multiple Transgenic Populus× euramericana 'Guariento’
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摘要: 以库安托杨转多基因株系(D5-9、D5-18、D5-19、D5-20、D5-21、D5-24、D5-26)和未转化株系(对照CK)为试材,研究不同水分处理(水涝胁迫、浸渍胁迫、正常供水)对其生长及生理性状的影响。结果表明:随着胁迫的加剧,各株系株高、根系生长和生物量累积呈下降趋势,而地径生长有所增加。浸渍、水涝胁迫下对照株系的净光合速率均最低,分别为11.99、10.37 μmol·m-2·s-1,比正常供水分别减少了14.0%和25.6%;浸渍胁迫下D5-26的净光合速率最高,为13.95 μmol·m-2·s-1;水涝胁迫下D5-19的净光合速率最高为12.01 μmol·m-2·s-1。蒸腾速率、水分利用效率、气孔限制值、叶绿素a、叶绿素b和总叶绿素含量均下降,叶绿素a的降幅小于叶绿素b,叶绿素a/b值先降后升,而气孔导度和细胞间隙CO2浓度增大。PSⅡ的实际光化学效率、电子传递速率、潜在光化学活性和最大光化学效率均降低,初始荧光产量和最大荧光产量增加。涝渍胁迫对所有供试材料的生长及光合、叶绿素荧光等生理性状均有影响,但对转多基因株系的影响明显较对照小,且株系间存在差异。以生长、光合参数和叶绿素荧光参数为指标对供试材料进行综合评价,各株系的抗涝能力从高到低的排序为:D5-26>D5-19>D5-18>D5-21>D5-9>D5-20>D5-24>CK。Abstract: Effects of different water treatments(waterlogging stress, light-waterlogging stress,normal water supply)on growth and physiology of multiple transgenic Populus× euramericana 'Guariento’(D5-9,D5-18,D5-19,D5-20,D5-21,D5-24,D5-26)were studied. The results showed that: Along with waterlogging stress intensifying, high growth, root growth and biomass accumulation of the multiple transgenic plants decreased, and the diameter of stem increased. Net photosynthetic rate decreased: The minimum was CK in the light-waterlogging stress treatment (11.99 μmol·m-2·s-1,14.0% lower than normal water supply) and the waterlogging stress treatment (10.37 μmol·m-2·s-1,25.6 % lower than normal water supply), whereas the maximum was D5-26(13.95 μmol·m-2·s-1) in the light-waterlogging stress treatment and D5-19(12.01 μmol·m-2·s-1) under waterlogging stress.Transpiration rate, water use efficiency, stomatal limit, chlorophyll a content, chlorophyll b content, total chlorophyll content, PSⅡ photochemical efficiency, electron transfer rate, potential fluorescence efficiency and maximum fluorescence efficiency of the multiple transgenic plants all decreased, but the reduction of chlorophyll a content was lower than chlorophyll b content. However, stomatal conductance, intercellular CO2, minimal fluorescence and maximum fluorescence increased. Waterlogging stress suppressed the growth, photosynthesis and chlorophyll fluorescence, and effects of the multiple transgenic plants were lighter and different. Growth, photosynthesis and chlorophyll fluorescence parameters were used to comprehensive evaluation of all the plants, the sequence of waterlogging resistance ability was: D5-26>D5-19>D5-18>D5-21>D5-9>D5-20>D5-24>CK.
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Key words:
- poplar
- / multiple transgenic
- / waterlogging
- / growth
- / photosynthesis
- / chlorophyllⅡ fluorescence
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