接种不同真菌菌株后黄栌生物电指标的变化
Changes in Bioelectrical Indexes of Cotinus coggygria var. cinerea Inoculated with Different Fungus Strains
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摘要: 以黄栌枯萎病发病植株的根部所分离到的3种优势菌株作为供试菌株,利用新型电感电容表和电导率仪分别测定黄栌的树干电容、电阻和叶片电导率等生物电指标的变化,发现接种不同真菌菌株后黄栌的树干电容均值都呈现逐渐下降的趋势,树干阻抗均值、叶片细胞外渗液的相对电导率和叶片离子外渗百分率则都呈现逐渐增大的趋势。但接种Verticillium dahliae后黄栌出现枯萎病症状,且各项生物电指标均呈现更为显著的变化,其变化幅度较分别接种Fusarium sp.、Cylindrosporium sp.菌株后的电指标变化幅度大,而且树干电容和电阻在接种5 - 7天后表现出显著差异性,比病害症状出现提前7 - 10天;同时,受Verticillium dahliae侵染的植株和未受侵染植株在树体干部电容、阻抗和单位电容指标上差异显著,树干电阻与树干电容在植株受侵染后表现出更为显著的负相关关系。Abstract: The paper studied the changes in bioelectrical indexes of Cotinus coggygria var. cinerea inoculated with three fungus strains isolated from infected C. coggygria var. cinerea, i.e. Verticillium dahliae, Fusarium sp. and Cylindrosporium sp., and the bioelectrical indexes, capacitance, impedance and conductivity, were tested by using the capacitance meter and electric conductivity instruments. The results indicated that the differences for the changes in bioelectrical indexes after inoculating with different fungus strains were significant,and the tree stem impedance, leaf exosmosis bioelectricity and exosmosis percentage increased gradually, while the tree stem capacitance decreased with the development of the process of infection. However, compared with inoculating with Fusarium sp., and Cylindrosporium sp., the ranges of changes in bioelectrical indexes of C. coggygria var. cinerea inoculated with V.dahliae, the main pathogens infecting C.coggygria var. cinerea, were larger, and also the changes of the tree stem impedance and tree stem capacitance showed more significant differences in 5 - 7 days after inoculation, which were 7 - 10 days earlier than the appearance of symptoms. There were evident differences between healthy and debility trees in stem impedance, tree stem capacitance and unit capacitance, and the stem impedance had more significant negative correlation with stem capacitance in infected C.coggygria var. cinerea.
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Key words:
- capacitance
- / impedance
- / conductivity
- / Verticillium wilt
- / Cotinus coggygria var. cinerea
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