毛竹花期不同器官内源激素含量的变化
Changes of Endogenous Hormones in Different Organs during the Flowering Phase of Moso Bamboo
-
摘要: 用酶联免疫法测定未开花毛竹和开花毛竹叶、花、枝、秆和鞭中内源激素吲哚乙酸(IAA)、赤霉素(GA3)、脱落酸(ABA)和玉米素核苷(ZR)的含量,分析开花毛竹内源激素分布和含量的变化与花形态分化的关系。结果表明:与未开花毛竹相比,成花期(6月中旬)开花毛竹叶、枝和秆中的IAA含量显著降低,枝的ABA含量也显著降低,而秆的ZR含量显著升高;扬花期(7月中旬)开花毛竹枝中的IAA含量显著降低,而GA3含量则显著升高。内源激素在开花毛竹各器官中的分布规律:IAA为秆>鞭>枝>花,GA3为花>鞭>枝>秆,ZR为花>枝>秆>鞭,ABA为秆>枝>花>鞭。二级枝中IAA含量显著低于一级枝,而其GA3和ZR的含量高于一级枝。IAA、GA3和ABA在竹杆中的含量呈向基性增加,而ZR则呈向基性减少。在成花期和扬花期ABA、ZR和GA3所占比例高于未开花毛竹,高比例的ABA、ZR和GA3和低比例的IAA可能促进毛竹花的发育。Abstract: Endogenous hormone contents of indole-3-acetic acid (IAA), gibberellin A3 (GA3), abscisic acid (ABA) and zeatin riboside(ZR)were measured by enzyme-linked immunosorbent assay(ELISA)in leaves, inflorescence, branches, stems and rhizomes of flowering and non-flowering moso bamboo(Phyllostachys edulis), for discussing the differences and understanding the relation between endogenous hormones distribution and contents and flower’s morphological differentiation. The results showed that: in comparison with non-flowering moso bamboo, the content of IAA in the leaves, branches and stems of flowering bamboo in Heading stage(mid-June)reduced significantly, and the content of ABA in the branches brought down significantly too, but the content of ZR in stems increased significantly. The content of IAA in the branches of flowering bamboo in flowering period(mid-July)was lower significantly and the content of GA3 rose significantly. The distribution characters of hormone in different organs were found different, while that of IAA was stem>rhizome>branch>inflorescence, that of GA3 was inflorescence>rhizome>branch>stem, that of ZR was inflorescence>branch>stem>rhizome and that of ABA was stem>branch>inflorescence>rhizome. The content of IAA in secondary side branches was significantly lower than that in primary side branches, but the contents of GA3 and ZR were higher than that in the primary side branches. The contents of IAA, GA3 and ABA in bamboo stem increased basipetally, while that of ZR in stem decreased basipetally. High rates of ABA, ZR and GA3 and low proportion of IAA may be helpful to the flower development during flower development, because ABA, ZR and GA3 had higher proportion in Heading stage and flowering period.
-
Key words:
- morphological differentiation
- / endogenous hormones
- / flower
- / moso bamboo
-
[1] Wong K M. Flowering, fruiting and germination of the bamboo Schizostachyum zollingeri in Perlis[J]. Malaysian Forester, 1981, 44(4): 453-463 [2] Keeley J E, Bond W J. Mast flowering and semelparity in bamboos: The bamboo fire cycle hypothesis[J]. American Naturalist, 1999, 154(3): 383-391 [3] Franklin D C. Synchrony and asynchrony: observations and hypotheses for the flowering wave in a long-lived semelparous bamboo[J]. J Biogeography, 2004, 31(5): 773-786 [4] Lin X C, Chow T Y, Chen H H, et al. Understanding bamboo flowering based on large-scale analysis of expressed sequence tags[J]. Genet Mol Res, 2010, 9(2):1085-1093 [5] Isagi Y, Shimada K, Kushima H, et al. Clonal structure and flowering traits of a bamboo stand grown from a simultaneous flowering as revealed by AFLP analysis[J]. Molecular Ecology, 2004, 13(7): 2017-2021 [6] 乔士义. 毛竹开花生物学特性的观察[J]. 竹类研究, 1984(2): 20-24 [7] 林树燕, 毛高喜. 竹子开花习性和开花竹林的更新[J]. 林业科技, 2007, 32(5): 23-26 [8] 周芳纯. 中国竹文化概要[J]. 竹类研究, 1998(1): 291-297 [9] 李秀菊, 孟繁静. 大豆品种早12花序分化期间的内源植物激素变化[J]. 作物学报, 1997, 23(4): 446-449 [10] 王兆龙, 曹卫星, 戴廷波. 小麦小花两极分化中内源植物激素与糖氮含量的变化特征[J]. 作物学报,2001, 27(4): 447-452 [11] 杨同文, 李潮海. 玉米性别决定的激素调控[J]. 植物学报, 2012, 47(1): 65-73 [12] 苏 华, 徐 坤, 刘 伟. 大葱花芽分化过程中内源激素的变化[J]. 园艺学报, 2007, 34(3): 671-676 [13] 丁兴萃. 覆盖栽培早竹开花的激素机制研究[J]. 林业科学, 2007, 43(7): 10-15 [14] 柴振林, 华锡奇, 王 琦. 雷竹花期激素动态及其与开花相关性研究[J]. 浙江林业科技, 2005, 25(1):58-64 [15] 鲁亚婷, 袁晓亮, 林新春, 等. 雷竹花芽形态分化过程中内源激素的变化规律[J]. 浙江农林大学学报, 2012, 29(2):161-165 [16] 吴良如, 萧江华. 大小年毛竹林中内源激素节律变化特征的研究[J]. 竹子研究汇刊, 1998, 17(1): 24-30 [17] 杨传平, 刘桂丰, 姜 静, 等. 白桦雄花发育过程中内源激素含量的变化[J]. 东北林业大学学报, 2002, 30(4): 1-4 [18] 何 见, 蒋丽娟, 李昌珠, 等. 光皮树花芽分化过程中内源激素含量变化的研究[J]. 中国野生植物资源, 2009, 28(2): 41-45 [19] 曹卫星, 王兆龙, 戴廷波. 不同穗型小麦小花发育过程中幼穗和叶片内源激素水平的动态变化[J]. 植物学报, 2000, 42 (10): 1026-1032 -
点击查看大图
计量
- 文章访问数: 3327
- HTML全文浏览量: 214
- PDF下载量: 1431
- 被引次数: 0
下载:
