我国几种重要松毛虫基因组大小测定

张苏芳; 张真; 王鸿斌; 孔祥波; 罗基同; 杨忠武

我国几种重要松毛虫基因组大小测定

1.
国家林业局森林保护学重点实验室, 中国林业科学研究院森林生态环境与保护研究所, 北京 100091
2.
广西壮族自治区森林病虫害防治站, 广西南宁 530022
3.
广西壮族自治区桂林市森林病虫害防治检疫站, 广西桂林 541001
基金项目:
国家自然科学基金青年基金(31200492);中国林科院森林生态环境与保护研究所基本科研业务费专项资金项目(CAFRIFEEP201102-5)
中图分类号: S763

Genome Size Determination of Several Dendrolimus Species in China

1.
Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China
2.
Forest pest and disease control station of the Guangxi Zhuang Autonomous Region, Nanning 530022, Guangxi, China
3.
Forest pest and disease control station of Guilin city, the Guangxi Zhuang Autonomous Region, Guilin 541001, China
CLC number: S763

摘要: 本研究采用流式细胞术，以公鸡（Gallus domesticus）血红细胞DNA含量为标准，测定了我国南方几种重要松毛虫的基因组大小（或称C-值）。结果显示，马尾松毛虫（Dendrolimus punctatus）的基因组大小为563.36±7.26 Mb，其雌、雄2C DNA含量分别为1.175 6±0.016 4和1.128 6±0.013 4 pg；思茅松毛虫（Dendrolimus kikuchii）的基因组大小为719.30±9.70 Mb，其雌、雄2C DNA含量分别为1.449 2±0.021 2和1.442 8±0.018 4 pg；云南松毛虫（Dendrolimus houi）的基因组大小为586.82±8.94 Mb，其雌、雄2C DNA含量分别为1.244±0.018 2和1.156±0.018 4 pg。统计分析结果发现，思茅松毛虫基因组显著大于云南松毛虫和马尾松毛虫基因组，后两者的基因组也存在显著差异，但绝对值相差较小。3种松毛虫雌雄虫的基因组大小之间也存在显著的差异，雌虫基因组大于雄虫。几种松毛虫基因组大小的测定，为进一步比较其分子差异、构建基因组文库和基因组学研究奠定了基础。
- 流式细胞仪
- / 基因组大小
- / 松毛虫
- / 鸡血细胞
Abstract: The genome size of three important Dendrolimus species in South China were measured by flow cytometry, with chicken erythrocyte DNA content as control. The results showed that the genome size of D. punctatus was 563.36±7.26 Mb, and the 2C DNA content of female and male were 1.1756±0.0164 and 1.1286±0.0134 pg, respectively. The genome size of D. kikuchii was 719.30±9.70 Mb, and the 2C DNA content of female and male were 1.4492±0.0212 and 1.4428±0.0184 pg, respectively. The genome size of D. houi was 586.82±8.94 Mb, and the 2C DNA content of female and male were 1.244±0.0182 and 1.156±0.0184 pg, respectively. Statistical analysis indicated that the genome size of D. kikuchii was much greater than that of D. houi and D. punctatus, and the genome size of D. houi was a little larger than that of the D. punctatus. For all the three species, the genome size of the female was larger than that of the male. Genome size determinations of these Dendrolimus species can be used as the basis of further comparison among the molecular differentiation of these closely related and sympatric species, and also provide important information for genomic library construction and sequencing in the future.
- flow cytometry
- / genome size
- / Dendrolimus
- / chicken erythrocyte

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我国几种重要松毛虫基因组大小测定

Genome Size Determination of Several Dendrolimus Species in China

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我国几种重要松毛虫基因组大小测定

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Genome Size Determination of Several Dendrolimus Species in China

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我国几种重要松毛虫基因组大小测定

Genome Size Determination of Several Dendrolimus Species in China

计量

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我国几种重要松毛虫基因组大小测定

English Abstract

Genome Size Determination of Several Dendrolimus Species in China

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