云南野生黄牡丹PlbHLH3转录因子基因的克隆与表达

史倩倩; 周琳; 李奎; 王雁

云南野生黄牡丹PlbHLH3转录因子基因的克隆与表达

1.
中国林业科学研究院林业研究所, 国家林业局林木培育重点实验室, 北京 100091
基金项目:
"863"计划(SQ2010AA1000687008)、国家自然科学基金项目(31201654)
中图分类号: S685.11

Isolation and Expression of PlbHLH3 Transcription Factor Genes in Paeonia lutea

1.
Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
CLC number: S685.11

摘要: 以云南野生黄牡丹为试材,基于已构建的花瓣转录组数据库,筛选到了24个与花色素苷合成相关的bHLH转录因子蛋白同源性高的Unigene序列,命名为PlbHLH1~24。通过开放阅读框(ORF)的氨基酸序列比较和系统进化树分析,发现PlbHLH3可能参与调控花色素苷合成,其ORF包含一个 2 040 bp的开放阅读框,编码一个679个氨基酸的蛋白;其氨基酸序列与葡萄VvMYC1和苹果MdbHLH3的亲缘关系最近,相似性达60%以上。相对荧光定量PCR分析表明,PlbHLH3在黄牡丹和紫牡丹的不同组织中均有表达,在两者的花药,心皮和花瓣中的表达显著高于在萼片和叶片中的表达;PlbHLH3在紫牡丹的硬蕾期高丰度表达,而在黄牡丹的硬蕾期表达量最低,在其他4个时期的表达量显著或极显著高于在硬蕾期的表达量。本研究推测PlbHLH3参与黄牡丹花色素苷合成的调控作用,为今后深入探讨黄牡丹花色形成机制奠定理论基础。
- 黄牡丹
- / bHLH
- / 花色素合成
- / 基因克隆
- / 表达分析
Abstract: Twenty-four unigene sequences shared high homology with bHLH transcription factor protein involved in plant anthocyanin biosynthesis were obtained from previous-constructed tree peony (Paeonia lutea) petal transcriptome database and named as PlbHLH1~24. By amino acid sequence comparing and phylogenetic tree analysis, it showed that PlbHLH3 was considered to be related to regulate anthocyanin biosynthesis, contained a 2 040 bp ORF encoding 679 amino acid residues with typical bHLH structural domain; the predicted protein sequence of PlbHLH3 also shared high similarity with other bHLH transcription factor that related to anthocyanin biosynthesis such as VvMYC1 and MdbHLH3. Relative Real-Time PCR analysis indicated that PlbHLH3 expressed in different tissues of P. lutea and P. delavayi, the expression in carpel, anther and petal was significantly higher than that in sepal and leaf; PlbHLH3 reached the highest abundance at early stage of P. lutea,. For P. delavayi, the PlbHLH3 reached the lowest level at early stage, and was significantly or extremely significantly lower than that in other four stages. In conclusion, it is inferred that PlbHLH3 might associate with the regulatory of anthocyanin biosynthesis in P. lutea and this would provide the basis for insight into the molecular mechanisms underlying tree peony yellow flower pigmentation.
- Paeonia lutea
- / bHLH
- / anthocyanin biosynthesis
- / gene cloning
- / expression analysis

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云南野生黄牡丹PlbHLH3转录因子基因的克隆与表达

Isolation and Expression of PlbHLH3 Transcription Factor Genes in Paeonia lutea

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云南野生黄牡丹PlbHLH3转录因子基因的克隆与表达

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Isolation and Expression of PlbHLH3 Transcription Factor Genes in Paeonia lutea

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云南野生黄牡丹PlbHLH3转录因子基因的克隆与表达

Isolation and Expression of PlbHLH3 Transcription Factor Genes in Paeonia lutea

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云南野生黄牡丹PlbHLH3转录因子基因的克隆与表达

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Isolation and Expression of PlbHLH3 Transcription Factor Genes in Paeonia lutea

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