[1] |
Livak K, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J]. Methods, 2001, 25(4): 402-408. doi: 10.1006/meth.2001.1262 |
[2] |
刘正霞, 徐阳, 徐进梅, 等.不同引物及数据分析方法对定量PCR结果的影响[J].南京医科大学学报:自然科学版, 2009, 29(8): 1112-1117. |
[3] |
Croteau R, Ketchum R E, Long R M, et al. Taxol biosynthesis and molecular genetics[J]. Phytochem Rev, 2006, 5(1): 75-97. doi: 10.1007/s11101-005-3748-2 |
[4] |
杨艳芳, 刘洪伟, 邱德有.欧洲红豆杉AP2/ERF转录因子基因序列分析[J].生物资源, 2014, 36(4):54-59. |
[5] |
李艳艳, 杨艳芳, 王俊青, 等.红豆杉TcLBDs基因的克隆与表达分析[J].林业科学, 2015, 51(10): 126-133. |
[6] |
Nasiri J, Naghavi M R, Alizadeh H, et al. Seasonal-based temporal changes fluctuate expression patterns of TXS, DBAT, BAPT and DBTNBT genes alongside production of associated taxanes in Taxus baccata[J]. Plant Cell Rep, 2016, 35(5):1103-1119. doi: 10.1007/s00299-016-1941-y |
[7] |
Liao W F, Zhao S Y, Zhang M, et al. Transcriptome assembly and systematic identification of novel cytochrome P450s in Taxus chinensis[J]. Front Plant Sci, 2017, 8: 1468. doi: 10.3389/fpls.2017.01468 |
[8] |
Zhang M, Chen Y, Nie L, et al. Molecular, structural, and phylogenetic analyses of Taxus chinensis JAZs[J]. Gene, 2017, 620: 66-74. doi: 10.1016/j.gene.2017.04.005 |
[9] |
Ramirez-Estrada K, Osuna L, Moyano E, et al. Changes in gene transcription and taxane production in elicited cell cultures of Taxus × media and Taxus globosa[J]. Phytochemistry, 2015, 117:174-184. doi: 10.1016/j.phytochem.2015.06.013 |
[10] |
Nolan T, Hands R E, Bustin S A. Quantification of mRNA using real-time PCR[J]. Nat Protoc, 2006, 1(3): 1559-1582. doi: 10.1038/nprot.2006.236 |
[11] |
Udvardi M K, Czechowski T, Scheible W R. Eleven golden rules of quantitative RT-PCR[J]. Plant Cell, 2008, 20(7): 1736-1737. doi: 10.1105/tpc.108.061143 |
[12] |
Baker M. qPCR: quicker and easier but don't be sloppy[J]. Nat Methods, 2011, 8(3): 207-212. doi: 10.1038/nmeth0311-207 |
[13] |
王效维, 刘海霞, 杨颖丽, 等.低表达甘蓝花药发育相关基因小体积荧光定量反应体系的简历和可靠性分析[J].西北农业学报, 2011, 20(4): 111-115. doi: 10.3969/j.issn.1004-1389.2011.04.023 |
[14] |
李婷, 韩莹琰, 范双喜, 等.叶用莴苣荧光定量PCR体系的优化[J].中国农学通报, 2013, 29(13): 106-113. doi: 10.11924/j.issn.1000-6850.2012-3317 |