USE OF ISOZYME TO IDENTIFY THE INHERITANCE CHARACTERISTICS OF VARIOUS FORMS IN A PINUS THUNBERGIl PARL.PLANTATION

The pattern of isopreoxidase and isoestrase separated by gel electrophoresis were applied as "genetic marker" to study various forms collected from a P. thunbergii" plantation in Shandong province, which has been introduced for over 70 years and is near to a P. densiflora plantation.14 various isopreoxidase patterns have been found in 19 various forms of P. thunbergii, which show a combination of different degree and manner of both P. thunb and P. densif. A new form of P. thunb selected from those possesses a resistance to Matsucoccus matsumurae (Kwana) and Dendrolimus spectabilis Butter better than P. thunb. Its isopreoxidase pattern of the needles possesses complementary bands of both P.thunb and P.densif. Its isoestrase pattern of seeds is segregated into two parts:1/2 pattern of the new form and 1/2 pattern of P. thunb. All of these come to a conclusion that there are various forms of taxonomic hybrid of P. thunb and P. densif in the plantation.Key words:isozyme;various form;taxonomic hybrid;genetic marker