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Volume 3 Issue 4
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Article Navigation >  Forest Research  > 1990 >  3(4): 341-344

Citation:
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RESTRICTION ENDONUCLEASE ANALYSIS OF HYPHANTRIA CUNEA NPV

  • 1.

    The Research Institute of Forestry CAF

  • Received Date: 1988-11-02
  • Viral DNA was isolated directly from the nuclear polyhedrosis virus of Hyphantria cunea. Viral DNA showed a single band on the agarose gel. Digestion of HcNPV-DNA with EcoR Ⅰ, Pst Ⅰ, Bgl Ⅱ, and PVU Ⅱ resulted in 19, 22. 18 and 22 fragments on agarose gel electrophoresis respectively. The molecular weights of the fragments are determined with DNA-EcoRI and DNA-Hind Ⅲ fragments as reference, and using this method the mean molecular weight of HcNPV-DNA is calculated to be about 94.044×106 daltons.
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通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

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RESTRICTION ENDONUCLEASE ANALYSIS OF HYPHANTRIA CUNEA NPV

  • 1. The Research Institute of Forestry CAF
  • Received Date: 1988-11-02
  • Available Online: 2012-12-04

Abstract: Viral DNA was isolated directly from the nuclear polyhedrosis virus of Hyphantria cunea. Viral DNA showed a single band on the agarose gel. Digestion of HcNPV-DNA with EcoR Ⅰ, Pst Ⅰ, Bgl Ⅱ, and PVU Ⅱ resulted in 19, 22. 18 and 22 fragments on agarose gel electrophoresis respectively. The molecular weights of the fragments are determined with DNA-EcoRI and DNA-Hind Ⅲ fragments as reference, and using this method the mean molecular weight of HcNPV-DNA is calculated to be about 94.044×106 daltons.

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