CONSTRUCTION OF PAULOWNIA GENOME LIBRARY AND SUBCLONING OF THE SPECIAL DNA FRAGMENT HOMOLOGOUS TO ACTIN GENE

This work is concerned in the construction of genome library of a resistant strain of Paulownia tomentosa to mycoplasmalike organisms (MLO). Large random DNA fragments are joined to phage lambda EMBL3 vectors by its polylinker. The recombinant molecules are packaged into E. coli LE392 in vitro and amplified to establish a permanent library. The efficiency of 1.1×106 pfu/ug DNA was attained. Among them there are 98% recombinant. Using the chicken actin gene as a hybridization probe, a special clone from this library was screened. By restriction endonuclease cleavage analysis and hybridization experiments, a 3.9Kb DNA fragment homologous to actin gene was subcloned into pUC19.