Study on the Propagation Technique in Vitro for New Varieties of Narcissus tazetta var. chinensis Rome

In this paper,the propagation technique in vitro for the new varieties of Narcissus selected from N. tazetta var. chinensis Rome was reported. In order to conserve germplasm resources of Narcissus and achieve the level of mass production, we conducted a series of synthetical systematic experiments for 3 years. The result showed that the bulbils were induced on twin scales on MS medium containing 0~5 mg/L BA, 0~1 mg/L NAA and a little activated-carbon. The percentage of induction reached 70%. More bulblets could be obtained through subculture in the same medium. If the bulbils were transplanted in 1/2MS medium with 0.1mg/L IBA in shade-culture, the rooting rate could be raised in the dark to 79.3%. If in vitro bulbils were cultivated in the field they might flower after 3 years.