Influence of Testing Conditions on the Apperance of the Hyphostroma-Isoesterase Electrophoretogram of Beauveria bassiana

A study on the testing conditions of the hyphostroma-esterase electrophoretic pattern of Beauveria bassiana (Bals.) Vuill. was conducted. The experimental results showed that the esterase electrophoretic pattern was mainly effected by five testing factors: constituentes of culture medium, amount of culture medium used, cultural peroid for hyphostroma,amount of conidia inoculated and different solutions of extracting esterase. The total numbers and shade of the colour of the esterase-bands were changed under different conditions. On the other hand, some side effect caused by EDTA was observed. Therefore, we may make a conclusion that a stable esterase electrophoretic pattern used as a classification criterion for strains of Beauveria bassiana can be obtained under the suitable conditions.